Capsular hyaluronic acid of equine isolates of <i><scp>S</scp></i><i>treptococcus zooepidemicus</i> is upregulated at temperatures below 35°C

Velineni, Sridhar; Timoney, John F.

doi:10.1111/evj.12272

Cited by 5 publications

(2 citation statements)

References 33 publications

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“…Interestingly, we note that HTP232 has two hyaluronidase genes carried on two different promoters, and one hyaluronidase gene contains a 6 bp deletion. Previous studies revealed that reduced hyaluronidase leads to high levels of hyaluronate capsules and enhances resistance to phagocytosis (Wibawan et al, 1999;Velineni and Timoney, 2015). The enhanced hyaluronate lyase activity may affect the levels of hyaluronate capsules in S. equi HTP232 and lead to low levels of capsules.…”

Section: Discussionmentioning

confidence: 98%

Complete genome sequencing and comparative genomic analysis of three donkey Streptococcus equi subsp. equi isolates

Zhang,

Lv,

et al. 2023

Front. Microbiol.

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IntroductionStreptococcus equi subspecies equi (S. equi) is the causative agent of strangles, which is one of the most common and highly contagious respiratory infectious illnesses in horses. Streptococcus equi (S. equi) is a horse-specific pathogen that originated from the closely related zoonotic pathogen Streptococcus equi subspecies zooepidemicus (S. zooepidemicus). Despite decades of research, the movement of genetic material across host-restricted diseases remains a mystery.MethodsThree S. equi donkey isolates (HTP133, HTP232, and HT1112) were recently isolated from a strangles epidemic on donkey farms in China’s Xinjiang Province. In this study, we performed a comprehensive comparative analysis of these isolates using whole genome sequencing and compared them to the published genomic sequences of equine strain S. equi 4047 to uncover evidence of genetic events that shaped the evolution of these donkey S. equi isolates’ genomes.ResultsWhole genome sequencing indicated that both strains were closely related, with comparable gene compositions and a high rate of shared core genomes (1788-2004). Our comparative genomic study indicated that the genome structure is substantially conserved across three donkey strains; however, there are several rearrangements and inversions when compared to the horse isolate S. equi 4047. The virulence factors conveyed by genomic islands and prophages, in particular, played a key role in shaping the pathogenic capacity and genetic diversity of these S. equi strains. Furthermore, we discovered that the HT133 isolate had a strong colonization ability and increased motility; the HT1112 isolates had a significantly higher ability for antimicrobial resistance and biofilm formation, and the HT232 isolate gained pathogenic specialization by acquiring a bacteriophage encoding hyaluronate lyase.DiscussionIn summary, our findings show that genetic exchange across S. equi strains influences the development of the donkey S. equi genome, offering important genetic insights for future epidemiological studies of S. equi infection.

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Section: Discussionmentioning

confidence: 98%

Complete genome sequencing and comparative genomic analysis of three donkey Streptococcus equi subsp. equi isolates

Zhang,

Lv,

et al. 2023

Front. Microbiol.

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“…The cell-associated hyaluronic acid (HA) of SEZ was measured using a procedure inspired by existing methods, with some modifications ( Velineni and Timoney, 2015 ). In brief, 10 ml of SEZ culture in THB, grown at 37°C, was centrifuged at 8000 g for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus

Xie,

Zhang,

Guo

et al. 2024

Front. Cell. Infect. Microbiol.

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Streptococcus equi subsp. zooepidemicus (SEZ) is an opportunistic pathogen of both humans and animals. Quorum sensing (QS) plays an important role in the regulation of bacterial group behaviors. The aim of this study was to characterize the LuxS in SEZ and evaluate its impact on biofilm formation, pathogenesis and gene expression. The wild-type SEZ and its LuxS mutant (ΔluxS) were examined for growth, biofilm formation, virulence factors, and transcriptomic profiles. Our results showed that LuxS deficiency did not affect SEZ hemolytic activity, adhesion or capsule production. For biofilm assay demonstrated that mutation in the luxS gene significantly enhances biofilm formation, produced a denser biofilm and attached to a glass surface. RAW264.7 cell infection indicated that ΔluxS promoted macrophage apoptosis and pro-inflammatory responses. In mice infection, there was no significant difference in mortality between SEZ and ΔluxS. However, the bacterial load in the spleen of mice infected with ΔluxS was significantly higher than in those infected with SEZ. And the pathological analysis further indicated that spleen damage was more severe in the ΔluxS group. Moreover, transcriptomics analysis revealed significant alterations in carbon metabolism, RNA binding and stress response genes in ΔluxS. In summary, this study provides the first evidence of AI-2/LuxS QS system in SEZ and reveals its regulatory effects on biofilm formation, pathogenicity and gene expression.

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Streptococcus

Timoney¹

2022

Pathogenesis of Bacterial Infections in Animals

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Capsular hyaluronic acid of equine isolates of Streptococcus zooepidemicus is upregulated at temperatures below 35°C

Cited by 5 publications

References 33 publications

Complete genome sequencing and comparative genomic analysis of three donkey Streptococcus equi subsp. equi isolates

Complete genome sequencing and comparative genomic analysis of three donkey Streptococcus equi subsp. equi isolates

Characterization of AI-2/LuxS quorum sensing system in biofilm formation, pathogenesis of Streptococcus equi subsp. zooepidemicus

Streptococcus

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