Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia

Mwirigi, Martin; Nkando, Isabel; Olum, Moses; Attah-Poku, Samuel K.; Ochanda, Horace; Berberov, Emil M.; Potter, Andrew; Gerdts, Volker; Perez-Casal, José; Wesonga, Hezron; Soi, Reuben; Naessens, Jan

doi:10.1016/j.vetimm.2016.07.002

Cited by 10 publications

(9 citation statements)

References 34 publications

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“…Initial data on a glycoconjugated vaccine approach for M. mycoides subsp. mycoides show some good prospects and support our findings [13]. The important role of CPS in M. mycoides requires further investigation as to exactly how this bacterium uses its enzymatic machinery as a virulence trait to identify key components that can be targeted for the rational design of efficient subunit vaccines.…”

Section: Discussionsupporting

confidence: 75%

In vivo role of capsular polysaccharide in Mycoplasma mycoides

Jores

Schieck

Liljander

et al. 2018

The Journal of Infectious Diseases

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Section: Discussionsupporting

confidence: 75%

In vivo role of capsular polysaccharide in Mycoplasma mycoides

Jores

Schieck

Liljander

et al. 2018

The Journal of Infectious Diseases

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“…Sera from naïve and subunit vaccinated cattle were used to determine Se and Sp of Mmm vaccine antigens, while sera from naïve and CBPP infected cattle were used to determine Se and Sp of Mmm non-vaccine antigens. In this study, the number of animals per group (n = 8) was based on previous immunization trials [7,[33][34][35][36]. The resource equation method was used to show that our sample size was adequately powered to address the research question.…”

Section: Determination Of Cut-off Point Diagnostic Sensitivity and Specificity Of MMM Antigensmentioning

confidence: 99%

Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia

et al. 2020

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Background Mycoplasma mycoides subsp. mycoides ( Mmm ) is the causative agent of contagious bovine pleuropneumonia in cattle. A prototype subunit vaccine is being developed, however, there is currently no diagnostic test that can differentiate between infected cattle and those vaccinated with the prototype subunit vaccine. This study characterized Mmm proteins to identify potential antigens for use in differentiating infected from vaccinated animals. Results Ten Mmm antigens expressed as recombinant proteins were tested in an indirect ELISA using experimental sera from control groups, infected, and vaccinated animals. Data were imported into R software for analysis and drawing of the box and scatter plots while Cohen’s Kappa assessed the level of agreement between the Mmm antigens. Two vaccine antigens (MSC_0499 and MSC_0776) were superior in detecting antibodies in sera of animals vaccinated with the subunit vaccines while two non-vaccine antigens (MSC_0636 and LppB) detected antibodies in sera of infected animals showing all clinical stages of the disease. Sensitivity and specificity of above 87.5% were achieved when the MSC_0499 and MSC_0636 antigens were tested on sera from vaccinated and infected animals. Conclusions The MSC_0499 and MSC_0776 antigens were the most promising for detecting vaccinated animals, while MSC_0636 and LppB were the best targets to identify infected animals. Further testing of sera from vaccinated and infected animals collected at different time intervals in the field should help establish how useful a diagnostic test based on a cocktail of these proteins would be.

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“…The pathogenesis of CBPP is considered multifactorial but remains largely unknown [ 7 – 9 ]. The capsular polysaccharide seems to promote Mmm adhesion to the host cells, enhancing the resistance to phagocytosis and playing a cytopathic effect on blood vessels [ 6 , 10 , 11 ]. The production of reactive oxygen species (ROS), the respiratory burst in phagocytic cells and immune-mediated mechanisms are supposed to further contribute to Mmm pathogenicity [ 12 – 15 ].…”

Section: Introductionmentioning

confidence: 99%

Respiratory explants as a model to investigate early events of contagious bovine pleuropneumonia infection

Teodoro

Marruchella

Provvido

et al. 2018

Vet Res

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Contagious bovine pleuropneumonia (CBPP) is a severe disease caused by Mycoplasma mycoides subsp. mycoides (Mmm). Knowledge on CBPP pathogenesis is fragmented and hampered by the limited availability of laboratory animal and in vitro models of investigation. The purpose of the present study is to assess respiratory explants as useful tools to study the early stages of CBPP. Explants were obtained from trachea, bronchi and lungs of slaughtered cattle, tested negative for Mycoplasma spp. and for the major bacterial and viral respiratory pathogens. The interaction of Mmm with explant cells was studied by immunohistochemistry (IHC), double-labelling indirect immunofluorescence (DLIIF) and laser scanning confocal microscopy (LSCM). Mmm capability to survive and proliferate within the explants was evaluated by standard microbiological procedures. Finally, the putative cellular internalization of Mmm was further investigated by the gentamicin invasion assay. IHC and DLIIF indicated that Mmm can colonize explants, showing a marked tropism for lower airways. Specifically, Mmm was detected on/inside the bronchiolar and alveolar epithelial cells, the alveolar macrophages and the endothelial cells. The interaction between Mmm and explant cells was abolished by the pre-incubation of the pathogen with bovine anti-Mmm immune sera. Mmm was able to survive and proliferate in all tracheal, bronchial and lung explants, during the entire time course of the experiments. LSCM and gentamicin invasion assay both confirmed that Mmm can enter non-phagocytic host cells. Taken together, our data supports bovine respiratory explants as a promising tool to investigate CBPP, alternative to cattle experimental infection.Electronic supplementary materialThe online version of this article (10.1186/s13567-017-0500-z) contains supplementary material, which is available to authorized users.

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Capsular polysaccharide from Mycoplasma mycoides subsp. mycoides shows potential for protection against contagious bovine pleuropneumonia

Cited by 10 publications

References 34 publications

In vivo role of capsular polysaccharide in Mycoplasma mycoides

In vivo role of capsular polysaccharide in Mycoplasma mycoides

Baseline analysis of Mycoplasma mycoides subsp. mycoides antigens as targets for a DIVA assay for use with a subunit vaccine for contagious bovine pleuropneumonia

Respiratory explants as a model to investigate early events of contagious bovine pleuropneumonia infection

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