Capsular polysaccharide switching in<i>Streptococcus suis</i>modulates host cell interactions and virulence

Okura, Masatoshi; Auger, Jean‐Philippe; Shibahara, Tomoyuki; Goyette-Desjardins, Guillaume; Calsteren, Marie‐Rose Van; Maruyama, Fumito; Kawai, Mikihiko; Osaki, Makoto; Segura, Mariela; Gottschalk, Marcelo; Takamatsu, Daisuke

doi:10.1101/2020.10.12.336958

Cited by 3 publications

(1 citation statement)

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“…Therefore, acquiring NeuB may increase bacterial resistance to complement system and phagocytosis (Feng et al, 2012; Uchiyama et al, 2019). In fact, a recent study highlighted that experimentally switching capsule type 2 to 3 in S. suis leads to defective whole blood survival and bacterial virulence (Okura et al, 2020). More studies in the future are needed to clarify the phenotypic features of different S. suis capsules, and what benefits bacteria may obtain from capsule switch.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic analyses provide clues to capsule switch inStreptococcus suis

Zhu

Wang

et al. 2020

Preprint

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Streptococcus suis (S. suis) is a major bacterial pathogen in swine industry and also an emerging zoonotic agent. S. suis produces an important extracellular component, capsular polysaccharides (CPS). Based on which, dozens of serotypes have been identified. Through virulence genotyping, we uncovered the relatedness between proportions of SS2, SS3 and SS7 strains despite their differences in serotypes. Multi-locus sequence typing (MLST) was used to characterize whole S. suis population, revealing that there is capsule switch between S. suis strains. Importantly, capsule switch occurred in SS2, 3 and 7 strains belonging to CC28 and CC29, which is phylogenetically distinct from the main CC1 SS2 lineage. To further explore capsule switch in S. suis, comparative genomic analyses were performed using available S. suis complete genomes. Phylogenetic analyses suggested that SS2 strains can be divided into two clades (1 and 2), and those classified into clade 2 are colocalized with SS3 and SS7 strains, which is in accordance with above virulence genotyping and MLST analyses. Clade 2 SS2 strains presented high genetic similarity with SS3 and SS7 and shared common competence and defensive elements, but are significantly different from Clade 1 SS2 strains. Notably, although the cps locus shared by Clade 1 and 2 SS2 strains is almost the same, a specific region in cps locus of strain NSUI002 (Clade 2 SS2) can be found in SS3 cps locus, but not in Clade 1 SS2 strain. These data indicated that SS2 strains appeared in CC28 and CC29 might acquire cps locus through capsule switch, which could well explain the distinction of genetic lineages within SS2 population.

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Section: Discussionmentioning

confidence: 99%

Comparative genomic analyses provide clues to capsule switch inStreptococcus suis

Zhu

Wang

et al. 2020

Preprint

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Screening of Streptococcus suis in swine workers of selected states in Peninsular Malaysia

Lee,

Zakaria,

Selvarajah

et al. 2024

Vet World

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Background and Aim: Streptococcus suis is a zoonotic pathogen that is highly associated with contact between live pigs and raw pig material. In view of the recent reports of human infections in Malaysia, epidemiological data on the status of S. suis in the human population, especially among people working closely with pigs and/or raw pork, should be provided. The aim of this study was to detect S. suis among individuals working in the swine industry in several major pig production areas in Peninsular Malaysia. Materials and Methods: Demographic information, exposure determinants, and oral swabs were collected from swine personnel, including farmers, butchers, and veterinarians. Oral swabs were subjected to bacterial isolation and conventional polymerase chain reaction (PCR) assays for S. suis detection. Results: The study included 40 participants working in the swine industry, with a predominant representation of males (62.5%) and Malaysian Chinese individuals (60.0%) who consumed pork (92.5%). Notably, none of the participants reported consuming raw or partially cooked pork. In spite of their occupational exposure risk, none of the oral swabs showed positive results for S. suis infection. Conclusion: To the best of our knowledge, this is the first report and detection study of S. suis using oral swabs obtained from swine personnel in Peninsular Malaysia. Keywords: carrier, high-risk occupation, human, Streptococcus suis.

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Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

Yan

Yao

Pan

et al. 2023

Veterinary Sciences

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Streptococcus suis is a significant pathogen in pigs and a newly emerging zoonotic agent in humans. The presence of multiple serotypes and strains with diversified sequence types in pig herds highlights the need for the identification of broadly cross-reactive universal vaccine antigen targets, capable of providing cross-protection against S. suis infection. Subunit vaccines based on the conserved proteins shared between different S. suis serotypes are potential candidates for such a universally protective vaccine. In the present study, phosphate ABC transporter ATP-binding protein PstB (PstB), an immunogenic protein of the S. suis bacterium, was expressed and purified, and then subjected to cross-protection evaluation in mice. The PstB protein showed nearly 100% amino acid similarity across a panel of 31 S. suis isolates representing different serotypes, which were collected from different countries. A recombinant PstB (rPstB) protein (S. suis serotype 2) was recognized by rabbit sera specific to this serotype, and induced high levels of IFN-γ and IL-4 in mice immunized with the recombinant protein. These cytokines are considered important for protection against S. suis infection. Immunization of mice with rPstB resulted in an 87.5% protection against challenge with S. suis serotype 2 and 9 strains, suggesting a high level of cross-protection for S. suis serotypes 2 and 9. A lower protection rate (62.5%) was observed in mice challenged with the S. suis serotype 7 strain. These data demonstrate that PstB is a promising target antigen for development as a component of a universal subunit vaccine against multiple S. suis serotypes.

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Capsular polysaccharide switching inStreptococcus suismodulates host cell interactions and virulence

Cited by 3 publications

References 38 publications

Comparative genomic analyses provide clues to capsule switch inStreptococcus suis

Comparative genomic analyses provide clues to capsule switch inStreptococcus suis

Screening of Streptococcus suis in swine workers of selected states in Peninsular Malaysia

Subunit Vaccine Targeting Phosphate ABC Transporter ATP-Binding Protein, PstB, Provides Cross-Protection against Streptococcus suis Serotype 2, 7, and 9 in Mice

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