Capsule Locus Polymorphism among Distinct Lineages of Enterococcus faecalis Isolated from Canals of Root-filled Teeth with Periapical Lesions

Pinheiro, Éricka Tavares; Penas, Pâmela P.; Endo, Marcos Sérgio; Gomes, Brenda Paula Figueiredo de Almeida; Mayer, Márcia Pinto Alves

doi:10.1016/j.joen.2011.08.002

Cited by 18 publications

(16 citation statements)

References 22 publications

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“…E. faecalis CPS type 2 was the most common serotype isolated from the samples collected in this study, indicating that the E. faecalis strain that produces capsules is highly prevalent in the human oral cavity in the Indonesian population. In contrast, in a study of E. faecalis isolated from root canals in a Brazilian population, E. faecalis CPS 2 strains were rare, and CPS 1 was the most common isolate . One explanation for this could be that there are geographic differences, because the varying oral environmental conditions could lead to genetic determinants of microbial colonization, including colonization by E. faecalis .…”

Section: Discussionmentioning

confidence: 92%

Enterococcus faecalis with capsule polysaccharides type 2 and biofilm‐forming capacity in Indonesians requiring endodontic treatment

Bachtiar

Dewiyani

Akbar

2015

J of Invest & Clin Dent

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Section: Discussionmentioning

confidence: 92%

Enterococcus faecalis with capsule polysaccharides type 2 and biofilm‐forming capacity in Indonesians requiring endodontic treatment

Bachtiar

Dewiyani

Akbar

2015

J of Invest & Clin Dent

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“…Surface capsules may prevent access of externally applied lysins to the bacterial cell wall, thereby precluding peptidoglycan hydrolysis. Capsule production is a frequent feature of oral E. faecalis strains, such as TUSoD11, isolated from root canals (55)(56)(57). It is conceivable that the peptidoglycan of TUSoD11 (and other seemingly resistant strains) is sensitive to the lysin but is protected from its action by the presence of a surface capsule.…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage φEf11 ORF28 Endolysin, a Multifunctional Lytic Enzyme with Properties Distinct from All Other Identified Enterococcus faecalis Phage Endolysins

Zhang

Buttaro

Fouts

et al. 2019

Appl Environ Microbiol

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Ef11 is a temperate Siphoviridae bacteriophage that infects strains of Enterococcus faecalis. The Ef11 genome, encompassing 65 open reading frames (ORFs), is contained within 42,822 bp of DNA. Within this genome, a module of six lysis-related genes was identified. Based upon sequence homology, one of these six genes, ORF28, was predicted to code for an N-acetylmuramoyl-Lalanine amidase endolysin of 46.133 kDa, composed of 421 amino acids. The PCRamplified ORF28 was cloned and expressed, and the resulting gene product was affinity purified to homogeneity. The purified protein was obtained from a fusion protein that exhibited a molecular mass of 72.5 kDa, consistent with a 46.1-kDa protein combined with a fused 26.5-kDa glutathione S-transferase tag. It produced rapid, profound lysis in E. faecalis populations and was active against 73 of 103 (71%) E. faecalis strains tested. In addition, it caused substantial destruction of E. faecalis biofilms. The lysin was quite stable, retaining its activity for three years in refrigerated storage, was stable over a wide range of pHs, and was unaffected by the presence of a reducing agent; however, it was inhibited by increasing concentrations of Ca 2ϩ . Liquid chromatography-mass spectrometry analysis of E. faecalis cell wall digestion products produced by the ORF28 endolysin indicated that the lysin acted as an N-acetylmuramidase, an endo-␤-N-acetylglucosaminidase, and an endopeptidase, rather than an N-acetylmuramoyl-L-alanine amidase. The Ef11 ORF28 lysin shared 10% to 37% amino acid identity with the lytic enzymes of all other characterized E. faecalis bacteriophages. IMPORTANCE The emergence of multidrug-resistant pathogenic microorganisms has brought increasing attention to the urgent need for the development of alternative antimicrobial strategies. One such alternative to conventional antibiotics employs lytic enzymes (endolysins) that are produced by bacteriophages in the course of lytic infection. During lytic infection by a bacteriophage, these enzymes hydrolyze the cell wall peptidoglycan, resulting in the lysis of the host cell. However, external endolysin application can result in lysis from without. In this study, we have cloned, expressed, purified, and characterized an endolysin produced by a bacteriophage infecting strains of Enterococcus faecalis. The lysin is broadly active against most of the tested E. faecalis strains and exhibits multifunctional enzymatic specificities that differ from all other characterized endolysins produced by E. faecalis bacteriophages.

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“…Only seven groups of E. faecalis capsules have been identified, i.e. cpsC, cpsD, cpsE, cpsG, cpsI, cpsJ, and cpsK [15]. Genetically, the synthesis of polysaccharide capsule operon coding and a polymorphism locus was found in the clinical isolates of E. faecalis [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Effects of Sarang Semut (Myrmecodia Pendans) Fractions Using Three Different Solvents Toward Enterococcus Faecalis Cps2

et al. 2020

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Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane),semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2.Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groupsof solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using aphytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five differentconcentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% wasused as a positive control. The width of the inhibition zone was calculated.Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from then-hexane and water solvents in inhibiting the growth of E. faecalis cps2.Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of thefraction increased with an increase in concentration.

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Capsule Locus Polymorphism among Distinct Lineages of Enterococcus faecalis Isolated from Canals of Root-filled Teeth with Periapical Lesions

Cited by 18 publications

References 22 publications

Enterococcus faecalis with capsule polysaccharides type 2 and biofilm‐forming capacity in Indonesians requiring endodontic treatment

Enterococcus faecalis with capsule polysaccharides type 2 and biofilm‐forming capacity in Indonesians requiring endodontic treatment

Bacteriophage φEf11 ORF28 Endolysin, a Multifunctional Lytic Enzyme with Properties Distinct from All Other Identified Enterococcus faecalis Phage Endolysins

Antibacterial Effects of Sarang Semut (Myrmecodia Pendans) Fractions Using Three Different Solvents Toward Enterococcus Faecalis Cps2

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