Capsule Types of Klebsiella

Edwards, P. R.; Fife, Mary A.

doi:10.1093/infdis/91.1.92

Cited by 79 publications

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“…Although differing morphologically, the slime antigens resemble the capsular antigens chemically and serologically. For instance, the similarity in antigenic specificity of slime and capsules was demonstrated for a klebsiella strain by Edwards & Fife (1952) and for strains A 3 and A 3 (Sl) in the present investigation. We therefore suggest that the antigenic variants be classified immunologically in four groups only: 'K' forms which produce capsular or slime antigens (e.g.…”

Section: Discussionsupporting

confidence: 72%

The Distribution of Polysaccharide Production in Aerobacter and Escherichia Strains and its relation to Antigenic Character:With a note on the Influence of Potassium Deficiency upon Production of Polysaccharide by Aerobacter aerogenes

Edmunds

Duguid

Wilkinson

1954

Journal of General Microbiology

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SUMMARY: Strains of Aerobacter aerogenes, A. cloacae and Escherichia coli, includingmucoid capsulate, mucoid non-capsulate, smooth and rough variant forms, were grown on defined agar media. The polysaccharide content of the cultures was estimated by the anthrone method and related to the amount of growth as measured by the non-dialysable nitrogen content. Polysaccharide production, as measured by the polysaccharide : nitrogen ratio, was increased in all strains when growth was limited by a restricted supply of nitrogen source so that added carbohydrate remained in relative excess. A low incubation temperature, 15-20° instead of 3 5 O , influenced polysaccharide production differently in the various strains and species ; thus it greatly increased production in E. coli strains, but decreased it in A. cloacae strains grown on certain media. The various strains showed considerable differences in the amount of polysaccharide production and in the distribution of the polysaccharide between the different possible sites of accumulation : i.e. intracellular, capsular and as loose slime. The mucoid strains produced the largest amounts of polysaccharide, most of which was present extracellularly as capsuIes or slime; the smooth strains produced much smaller amounts, almost entirely intracellular; a rough strain produced least of all. The antigenic constitution of certain strains was examined, particularly that of a mucoid capsulate A. aerogenes and mucoid non-capsulate and non-mucoid smooth variants derived from it. The designation of such morphological and immunological variants is discussed.The amount of polysaccharide produced varies greatly with different strains and species of Enterobacteriaceae. Among twelve strains of Escherichia coli grown at 13-17' on glucose medium, Morgan & Beckwith (1939) found that the amount of polysaccharide produced, as indicated by the degree of mucoidness, was large in five strains, moderate in two strains and small in the remainder ; they found similar differences among salmonella strains. Mucoid, smooth and rough variants of the same strain may also show great differences in polysaccharide production. (1951), using the periodate-Schiff staining method, found that 0-inagglutinable rough variants of salmonella and shigella strains lacked the stainable intracellular polysaccharide of the smooth parent strains ; they therefore suggested that this polysaccharide corresponded to the hapten of the 0 antigen characteristic of the smooth form.Duguid & Wilkinson (1953) studied the influence of different cultural conditions on the amount of polysaccharide produced by a single strain (A3) of Aerobacter aerogenes, distinguishing the polysaccharide situated intracellularly, in the capsules or in the loose extracellular slime. In the present investigation the same methods have been applied for examination of several different strains of A . aerogenes, A . cloacae and Eschrichia coli. For each strain the effect was observed of variation in the sugar:nitrogen ratio of the medium and of variation in th...

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Section: Discussionsupporting

confidence: 72%

The Distribution of Polysaccharide Production in Aerobacter and Escherichia Strains and its relation to Antigenic Character:With a note on the Influence of Potassium Deficiency upon Production of Polysaccharide by Aerobacter aerogenes

Edmunds

Duguid

Wilkinson

1954

Journal of General Microbiology

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“…Merck standard I broth was generally used for liquid and solid media, and an additional 0.3% (wt/vol) of Glc was added for the large-scale propagation of phage. For the production of capsular 1 (14,33), the serological test strain for the Klebsiella K25 antigen (15,24,31), was used; it was kindly supplied by Ida Orskov, World Health Organization International Escherichia Center, Statens Seruminstitut, Copenhagen.…”

Section: Methodsmentioning

confidence: 99%

“…All these glycans are rather similarmost of them contain either D-glucuronic acid (GlcUA) or, occasionally, D-galacturonic acid, as well as two to four of the following aldohexoses: D-glucose (Glc), D-galactose (Gal), D-mannose, L-fucose, and L-rhamnose (31). Therefore, this set of polysaccharides is well suited to a variety of comparative studies: for instance, by nuclear magnetic resonance (e.g., reference 1), on the substrate specificity of bacteriophageborne glycanases (e.g., reference 42), on the chemical basis of the serological cross-reactions between Klebsiella K antigens (e.*, references 14,15,17) or between Klebsiella antigens and those of other bacterial genera (e.g., reference 18), or on polysaccharide conformation.…”

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confidence: 99%

Klebsiella serotype-13 capsular polysaccharide: Primary structure and depolymerization by a bacteriophage-borne glycanase

Niemann¹,

Frank²,

Stirm³

1977

Carbohydrate Research

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“…Following the pioneering work of Julianelle (17), who described the first three capsular serotypes, 77 K antigens are now included in the international K-typing scheme (27,28). Determination of the K type has been used since the 1920s to type Klebsiella isolates (17) and has long been the preferred method for the investigation of epidemiological relationships among strains (11,13,14,18,28). Because it is highly discriminatory and reproducible, K serotyping is particularly useful for the comparison of strains from different geographical locations and different times.…”

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confidence: 99%

Molecular Serotyping ofKlebsiellaSpecies Isolates by Restriction of the Amplified Capsular Antigen Gene Cluster

2004

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The objective of the present work was to develop a molecular method that would enable determination of the capsular serotypes of Klebsiella isolates without the use of antiserum. PCR amplification of the capsular antigen gene cluster (cps) was followed by digestion with the restriction enzyme HincII (cps PCR-restriction fragment length polymorphism [RFLP] analysis). The profiles (C patterns) obtained for 224 strains representing the 77 known K serotypes showed 3 to 13 fragments ranging in size from 0.2 to 4.4 kb. A total of 97 distinct C patterns were obtained; 100% of 61 pairs of samples tested twice showed reproducible C patterns. The C patterns were K-type specific; i.e., the C pattern(s) of any K serotype was distinct from the C patterns of all other K serotypes, with the only exceptions being serotypes K22 and K37, which are known to cross-react. For 12 of 17 K types for which at least two strains were included, C-pattern variations were found among strains with the same K serotype. Therefore, cps PCR-RFLP analysis has a higher discriminatory power than classical K serotyping. C-pattern identity was observed among strains with a given K type that were collected many years apart and from distinct sources, indicating C-pattern stability. Only 4.5% of the strains were nontypeable, because of unsuccessful PCR amplification (whereas 8 to 23% are nontypeable by classical K serotyping). Three of four noncapsulated strains analyzed showed recognizable C patterns. The K serotypes of 18 (82%) of 22 recent Klebsiella pneumoniae clinical isolates could be deduced from their C patterns. In conclusion, cps PCR-RFLP analysis allows determination of the K serotype, while it is easier to perform and more discriminatory than classical serotyping.

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Capsule Types of Klebsiella

Cited by 79 publications

References 0 publications

The Distribution of Polysaccharide Production in Aerobacter and Escherichia Strains and its relation to Antigenic Character:With a note on the Influence of Potassium Deficiency upon Production of Polysaccharide by Aerobacter aerogenes

The Distribution of Polysaccharide Production in Aerobacter and Escherichia Strains and its relation to Antigenic Character:With a note on the Influence of Potassium Deficiency upon Production of Polysaccharide by Aerobacter aerogenes

Klebsiella serotype-13 capsular polysaccharide: Primary structure and depolymerization by a bacteriophage-borne glycanase

Molecular Serotyping ofKlebsiellaSpecies Isolates by Restriction of the Amplified Capsular Antigen Gene Cluster

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