Capture and identification of bacteria from fish muscle based on immunomagnetic beads and MALDI-TOF MS

Chai, Zhaoliang; Bi, Hongyan

doi:10.1016/j.fochx.2022.100225

Cited by 13 publications

(11 citation statements)

References 39 publications

(43 reference statements)

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“…Immunomagnetic separation of target bacteria from food has been employed with a variety of other detection methods with high sensitivity. One recent study, for example, was capable of detecting E. coli from fish muscle at concentrations as low as 10 CFU/mL using IMS and mass spectrometry [ 84 ]. Other detection methods implementing IMS for bacteria concentration include fluorescence methods [ 83 ], surface plasmon resonance [ 57 , 85 ], microfluidics [ 86 ], and other biosensors [ 22 , 87 ].…”

Section: Immunomagnetic Separation Versus Glycan-coated Mnp Separationmentioning

confidence: 99%

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Dester

Alocilja

2022

Biosensors

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Rapid and accurate food pathogen detection is an essential step to preventing foodborne illnesses. Before detection, removal of bacteria from the food matrix and concentration to detectable levels are often essential steps. Although many reviews discuss rapid concentration methods for foodborne pathogens, the use of glycan-coated magnetic nanoparticles (MNPs) is often omitted. This review seeks to analyze the potential of this technique as a rapid and cost-effective solution for concentration of bacteria directly from foods. The primary focus is the mechanism of glycan-coated MNP binding, as well as its current applications in concentration of foodborne pathogens. First, a background on the synthesis, properties, and applications of MNPs is provided. Second, synthesis of glycan-coated particles and their theorized mechanism for bacterial adhesion is described. Existing research into extraction of bacteria directly from food matrices is also analyzed. Finally, glycan-coated MNPs are compared to the magnetic separation technique of immunomagnetic separation (IMS) in terms of cost, time, and other factors. At its current state, glycan-coated MNPs require more research to fully identify the mechanism, potential for optimization, and extraction capabilities directly in food matrices. However, current research indicates glycan-coated MNPs are an incredibly cost-effective method for rapid food pathogen extraction and concentration.

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Section: Immunomagnetic Separation Versus Glycan-coated Mnp Separationmentioning

confidence: 99%

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Dester

Alocilja

2022

Biosensors

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“…To obtain the optimal sensing performance, six experimental conditions, including polymerization cycles (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15), template concentration (10 7 -10 9 CFU mL −1 ), template elution (types of eluents and elution time from 5-15 min), recognition time (5-15 min), and oscillation speed during recognition (0-300 rpm) were optimized independently. The choice of the optimum experimental conditions, except template elution dependent on the degree of reduction in the R ct value, was based on the ΔR/R towards S. aureus at 10 5 CFU mL −1 .…”

Section: Optimization Of Experimental Conditionsmentioning

confidence: 99%

“…2,3 Moreover, S. aureus can rapidly adapt to antibiotics and evolve into antibiotic-resistant bacteria, such as methicillin-resistant S. aureus (MRSA), that have imposed huge burdens on medical care, and posed serious threats to human life. 4,5 Existing methods for tracking pathogenic bacteria such as cell culturing, 6 enzyme-linked immunosorbent assay (ELISA), 7,8 polymerase chain reaction (PCR), 9,10 mass spectrometry, 11,12 flow cytometry, 13 and surface-enhanced Raman scattering (SERS) 14,15 are accurate and reliable. Still, they are restricted by lengthy analysis times, insufficient sensitivity, complicated operation, or expensive instruments and reagents.…”

Section: Introductionmentioning

confidence: 99%

Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film

Wang

Lin

Liu

et al. 2022

Analyst

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“…MALDI-TOF MS has a high level of accuracy for species-level identification of bacteria that affect human and animal health ( 19 – 21 ). MALDI-TOF MS has replaced or supplemented phenotypic and molecular methods of identifying bacteria in modern-day clinical microbiology ( 22 – 24 ). Identification of bacteria to the species-level using MALDI-TOF MS is achieved by matching the spectral profile of bacterial peptides against existing main spectral profile (MSP) in the reference library (widely used in veterinary laboratories is the commercial MALDI Biotyper ® reference library manufactured by Bruker), which can be performed in < 1 h ( 25 , 26 ).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

et al. 2022

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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms. The accuracy of bacterial identification using MALDI-TOF MS depends on main spectral profiles (MSPs) provided in a quality-assured commercial reference library, which requires ongoing improvement. This study aimed to develop and validate an in-house MALDI-TOF MS MSP to rapidly identify Yersinia ruckeri isolated from Atlantic salmon (Salmo salar). The novel MSP was prepared using an isolate of Y. ruckeri recovered from Atlantic salmon and confirmed by 16S rRNA gene sequencing. Subsequently, a validation set which comprises 29 isolates of Y. ruckeri were examined from three fishes: Atlantic salmon (Salmo salar) (n = 26), American eel (Anguilla rostrata) (n = 1), and Atlantic cod (Gadus morhua) (n = 2). These isolates were randomly selected from the Atlantic Veterinary College, Aquatic Diagnostic Services Bacteriology Laboratory's culture collection to validate the novel MSP. Analytical sensitivity of MALDI-TOF MS using the novel MSP to identify the validation set was 86.2%. Repeatability was assessed by acquiring spectra from 30 different spots of a randomly-selected isolate of Y. ruckeri, and analyzed spectra from each spot were compared against the novel MSP. The coefficient of variation was 3.3%. The novel MSP clustered with Bruker MSPs (n = 3) of Y. ruckeri in the reference library and did not falsely identify any closely related bacteria to Y. ruckeri. This study reports the development of a novel MSP of high analytical sensitivity and specificity for rapid identification of Y. ruckeri using MALDI-TOF MS.

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Capture and identification of bacteria from fish muscle based on immunomagnetic beads and MALDI-TOF MS

Cited by 13 publications

References 39 publications

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Current Methods for Extraction and Concentration of Foodborne Bacteria with Glycan-Coated Magnetic Nanoparticles: A Review

Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film

Development and validation of main spectral profile for rapid identification of Yersinia ruckeri isolated from Atlantic salmon using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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