Capture and Stimulated Release of Circulating Tumor Cells on Polymer‐Grafted Silicon Nanostructures

Hou, Shuang; Zhao, Haichao; Zhao, Libo; Shen, Qinglin; Wei, Kevin; Suh, Daniel Y.; Nakao, Aiko; Garcia, Mitch A.; Song, Min; Lee, Tom; Xiong, Bin; Luo, Shyh‐Chyang; Tseng, Hsian‐Rong; Yu, Hsiao‐hua

doi:10.1002/adma.201203185

Cited by 243 publications

(242 citation statements)

References 29 publications

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“…Different strategies have emerged in recent decades for using materials that match the requirements for capturing CTCs in blood with efficiencies ranging from 50% to 99% [3][4][5][6]. Among them, methodologies based on Velcro-like devices [7,8], biomimetic nano-platforms [9], microfluidic devices such as CTC-chips [1,10,11], DEPArray systems [12] or CTC enrichment [13,14] have been developed.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

et al. 2018

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Magnetic nanogels (MNGs) are designed to have all the required features for their use as highly efficient trapping materials in the challenging task of selectively capturing circulating tumor cells (CTCs) from the bloodstream. Advantageously, the discrimination of CTCs from hematological cells, which is a key factor in the capturing process, can be optimized by finely tuning the polymers used to link the targeting moiety to the MNG. We describe herein the relationship between the capturing efficiency of CTCs with overexpressed transferrin receptors and the different strategies on the polymer used as linker to decorate these MNGs with transferrin (Tf). Heterobifunctional polyethylene glycol (PEG) linkers with different molecular weights were coupled to Tf in different ratios. Optimal values over 80% CTC capture efficiency were obtained when 3 PEG linkers with a length of 8 ethylene glycol (EG) units were used, which reveals the important role of the linker in the design of a CTC-sorting system.

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Section: Introductionmentioning

confidence: 99%

Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

et al. 2018

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“…CTCs provide an opportunity to noninvasively monitor cancer patients' prognosis and response to therapy. Over the past few decades, although great endeavor has been devoted to developing CTC capture and recovery devices in which captured cells are released usually under certain special stimuli (such as light, electric current, enzymes, temperature, and pH), [7][8][9][10][11] these techniques are often limited by a capture and recovery performance tradeoff between high efficiency and high viability. Hence, there is a desperate need for techniques to capture and recover high viability CTCs.…”

mentioning

confidence: 99%

“…Therefore, based on our previous studies, 7,8 we anticipated that further improvement of cell viability performance could be achieved by decreasing the heating/cooling cycles and enzymatic treatment rounds. Thermoresponsive poly (N-isopropylacrylamide) (PNIPAM) [15][16][17][18][19][20] is one of typical thermosensitive polymers with a good biocompatible and a lower critical solution temperature behavior approximately 32°C in an aqueous solution, which is close to the physiological temperature, so PNIPAM is widely applied to the bioseparation.…”

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confidence: 99%

“…Thermoresponsive SiNWS can capture and release CTCs at 37°C and 4°C, respectively. 7 The temperature-dependent conformational changes of polymer brushes can alter the accessibility of the capture agent (eg, anti-EpCAM) on the SiNWS. We also developed a platform that is capable of not only capturing CTCs with high efficiency but also recovering the CTCs with high purity and viability upon two rounds of nuclease treatment.…”

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confidence: 99%

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Aptamer-polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing

Shen

Peng

Zhan

et al. 2016

IJN

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Selection of the optimal chemotherapy regimen for an individual cancer patient is challenging. The existing chemosensitivity tests are costly, time-consuming, and not amenable to wide utilization within a clinic. This limitation might be addressed by the recently proposed use of circulating tumor cells (CTCs), which provide an opportunity to noninvasively monitor response to therapy. Over the past few decades, various techniques were developed to capture and recover CTCs, but these techniques were often limited by a capture and recovery performance tradeoff between high viability and high efficiency. In this work, we used anti-epithelial cell adhesion molecule coated aptamer–poly (N-isopropylacrylamide) functionalized silicon nanowire substrates to capture and release epithelial cell adhesion molecule-positive CTCs at 32°C and 4°C, respectively. Then, we applied the nuclease to digest the aptamer to release the captured CTCs (near or at the end of the polymer brush), which cannot be released by heating/cooling process. High viability and purity CTCs could be achieved by decreasing the heating/cooling cycles and enzymatic treatment rounds. Furthermore, the time-saving process is helpful to maintain the morphology and enhance vitality of the recovered CTCs and is beneficial to the subsequent cell culture in vitro. We validated the feasibility of chemosensitivity testing based on the recovered HCC827 cells using an adenosine triphosphate–tumor chemosensitivity assay, and the results suggested that our method can determine which agent and what concentration have the best chemosensitivity for the culturing recovered CTCs. So, the novel method capable of a highly effective capture and recovery of high viability CTCs will pave the way for chemosensitivity testing.

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“…Nanoneedles can detect and sort circulating cancer cells within biological fluids 30 , since their enhanced interaction enables more effective capture. Other notable efforts are those related to measuring intracellular conditions, such as pH 3 , which can provide an overall indication of cell metabolism, and the measurement of cell tension through the force they exert 17 .…”

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confidence: 99%

Nanoneedle-Based Sensing in Biological Systems

Chiappini

2017

ACS Sens.

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Nanoneedles are high aspect ratio nanostructures with a unique biointerface. Thanks to their peculiar yet poorly understood interaction with cells, they very effectively sense intracellular conditions, typically with lower toxicity and perturbation than traditionally available probes. Through long-term, reversible interfacing with cells, nanoneedles can monitor biological functions over the course of several days. Their nanoscale dimension and the assembly into large scale, ordered, dense arrays enable monitoring the functions of large cell populations, to provide functional maps with sub-micron spatial resolution. Intracellularly, they sense electrical activity of complex excitable networks, as well as concentration, function and interaction of biomolecules in situ, while extracellularly they can measure the forces exerted by cells with piconewton detection limits, or efficiently sort rare cells based on their membrane receptors. Nanoneedles can investigate the function of many biological systems, ranging from cells, to biological fluids, to tissues and living organisms. This review examines the devices, strategies and workflows developed to use nanoneedles for sensing in biological systems. KeywordsNanoneedles, nanowires, biointerface, intracellular sensing, review, biomarkers, label-free, minimally invasive.Nanoneedles are rapidly emerging as a tool to interact with the intracellular environment of large number of cells simultaneously, with limited perturbation of their physiological processes. This interaction provides characteristics advantages for minimally invasive cell and molecular biology investigations, as well as to progress biomedical translation of regenerative and precision medicine approaches. A quick string of several successful proofs of principles have established nanoneedles' potential to efficiently deliver impermeant molecules 1,2 and nanoparticles 3 directly to the cell cytosol, and to sense the intracellular milieu 4-6 across biological systems ranging from cells in culture 7 to living organisms 8 . Nanoneedles can be broadly defined as nanomaterials whose high aspect ratio enables their biological interaction; this definition encompasses a broad range of classically defined nanomaterials, which cater for different biointerfacing needs and applications ( Figure 1). Figure 1 Overview of the nanoneedle devices and strategies used for sensing in biological systems.Each nanoneedle is a stacked bar of a bar graph. The legend below each nanoneedle is color-coded and connects to the associated bar. The height of each bar represents the fraction of reviewed papers with that characteristic. The number in each bar is the number of reviewed publications with that characteristic. Numbers for each bar do not sum to the same total as publications can contribute to more than one bar in a given stack. *"Fluid" excludes biological fluids, + "in vivo/ex vivo" includes biological fluids. The overview is compiled from all the publications reviewed herein that include sensing work.Nanoneedles belong t...

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Capture and Stimulated Release of Circulating Tumor Cells on Polymer‐Grafted Silicon Nanostructures

Cited by 243 publications

References 29 publications

Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

Optimizing Circulating Tumor Cells’ Capture Efficiency of Magnetic Nanogels by Transferrin Decoration

Aptamer-polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing

Nanoneedle-Based Sensing in Biological Systems

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