Capture enrichment and massively parallel sequencing for human identification

Gorden, Erin M.; Sturk-Andreaggi, Kimberly; Marshall, Charla

doi:10.1016/j.fsigen.2021.102496

Cited by 22 publications

(10 citation statements)

References 56 publications

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“…Techniques for identification of human remains continue to improve particularly with the capabilities of NGS and hybridization capture [ 595 ] and ancient DNA extraction protocols [ 596 , 597 ]. Studies have reported variation in skeletal DNA preservation [ 598 ] and retrospectively considered success rates with compromised human remains [ 599 ].…”

Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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“…This method uses small hybridization probes ranging in length of base pairs and are assembled in panels of probes that can be as few as 50 probes up to millions of probes [ 149 ]. In the context of human genome sequencing and mutation panels, these hybridization probes are added to fragmented DNA and enrich for genes or mutations of interest [ 150 ]. This technology has been advanced for implementation in the clinical laboratory with the development of a bacterial probe hybridization panel known as a bacterial capture sequencing (BacCapSeq) system [ 151 ] and a viral probe hybridization panel known as Virome Capture Sequencing Platform for Vertebrate Viruses (VirCapSeq-VERT) [ 152 ].…”

Section: Direct Sample Metagenomics Sequencingmentioning

confidence: 99%

Next Generation and Other Sequencing Technologies in Diagnostic Microbiology and Infectious Diseases

Hilt

Ferrieri

2022

Genes

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Next-generation sequencing (NGS) technologies have become increasingly available for use in the clinical microbiology diagnostic environment. There are three main applications of these technologies in the clinical microbiology laboratory: whole genome sequencing (WGS), targeted metagenomics sequencing and shotgun metagenomics sequencing. These applications are being utilized for initial identification of pathogenic organisms, the detection of antimicrobial resistance mechanisms and for epidemiologic tracking of organisms within and outside hospital systems. In this review, we analyze these three applications and provide a comprehensive summary of how these applications are currently being used in public health, basic research, and clinical microbiology laboratory environments. In the public health arena, WGS is being used to identify and epidemiologically track food borne outbreaks and disease surveillance. In clinical hospital systems, WGS is used to identify multi-drug-resistant nosocomial infections and track the transmission of these organisms. In addition, we examine how metagenomics sequencing approaches (targeted and shotgun) are being used to circumvent the traditional and biased microbiology culture methods to identify potential pathogens directly from specimens. We also expand on the important factors to consider when implementing these technologies, and what is possible for these technologies in infectious disease diagnosis in the next 5 years.

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“…On the other hand, when working with the most highly degraded samples, DNA capture is an option. Forensic samples too degraded for conventional PCR techniques are candidates for capture as this method was developed for analyzing highly fragmented pieces of DNA (50 bp or less) (Eduardoff et al, 2017; Gorden et al, 2021; Loreille et al, 2018; Marshall et al, 2017; Marshall, Taylor, et al, 2020; Shih et al, 2018). Capture combined with MPS creates the potential to obtain genetic data from forensic samples too degraded for typical DNA analysis.…”

Section: Massively Parallel Sequencingmentioning

confidence: 99%

The time is now for ubiquitous forensicmtMPSanalysis

Canale

Parson

Holland

2021

WIREs Forensic Science

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Mitochondrial (mt) DNA sequence analysis is useful for assessing ancestral origin and migration, identifying human remains, and examining evidentiary material in forensic casework. Conventional Sanger‐type sequencing (STS) has been used for more than three decades to address these interests. This paper reviews the methodologies and merits of using a massively parallel sequencing (MPS) approach for mtDNA testing in forensic laboratories, as The Time is Now for Ubiquitous Forensic mtMPS Analysis. This article is categorized under: Forensic Biology > Haploid Markers Forensic Biology > Forensic DNA Technologies

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Capture enrichment and massively parallel sequencing for human identification

Cited by 22 publications

References 56 publications

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Next Generation and Other Sequencing Technologies in Diagnostic Microbiology and Infectious Diseases

The time is now for ubiquitous forensicmtMPSanalysis

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