Capture of Viable Circulating Tumor Cells in the Liver of Colorectal Cancer Patients

Denève, E.; Riethdorf, Sabine; Ramos, Jeanne; Nocca, David; Coffy, Amandine; Daurès, Jean‐Pierre; Maudelondé, Thierry; Fabre, Jean‐Michel; Pantel, Klaus; Alix‐Panabières, Catherine

doi:10.1373/clinchem.2013.202846

Cited by 199 publications

(144 citation statements)

References 39 publications

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“…Thus, CellSearch may overlook CTCs in cases of tumors that have undergone EMT and lack EpCAM expression. Furthermore, many of the CTCs that are detected by CellSearch are not viable, but apoptotic (9). Currently, no sensitive assays have been developed for the detection of viable CTCs (v-CTCs).…”

Section: Impact Of Endoscopic Stent Insertion On Detection Of Viable mentioning

confidence: 99%

“…CTCs in the peripheral blood samples of cancer patients were detected as previously described (12,13). Briefly, cells recovered from the 7.5-ml blood samples of OCRC patients were incubated with 1x10 9 VP of rAdF35-142T-GFP (TelomeScanF35) at 37˚C. Following 24-h incubation, the cells were washed and stained with anti-human CD45 antibody (clone HI30; Biolegend, San Diego, CA) and then observed by fluorescence microscopy.…”

Section: Detection Of Ctcs In the Blood Samples Of Ocrcmentioning

confidence: 99%

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Impact of endoscopic stent insertion on detection of viable circulating tumor cells from obstructive colorectal cancer

et al. 2017

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Abstract. The placement of a self-expanding metallic stent (SEMS) in obstructive colorectal cancer (OCRC) is acknowledged to be a safe and effective procedure for the relief of obstruction. However, there is concern that shear forces acting on the tumor during stent expansion may release cancer cells into the circulation, resulting in a poor prognosis. The aim of the present study was to determine whether colonic stent insertion increases viable circulating tumor cells (v-CTCs). A telomerase-specific replication-selective adenovirus-expressing GFP (TelomeScanF35) detection system was used to detect v-CTCs in 8 OCRC patients with a SEMS before and after stent insertion and after surgical resection. In 7 patients, a SEMS was inserted as a bridge to surgery (BTS), and in one patient, a SEMS was inserted for palliation. Surgical resection (R0) was performed in 7 patients. Four patients had no v-CTCs before SEMS placement, two of four measurable patients had an increased number of v-CTCs after SEMS placement (1-3 v-CTCs), and one of two patients with increased v-CTCs developed distant lymphatic metastasis despite curative resection. Four patients had v-CTCs (1-19 cells) before SEMS placement, and two of these four patients had an increase in the number of v-CTCs (20-21 cells) after SEMS placement, while one of the four patients died early with distant metastasis. The present study demonstrated that endoscopic stent insertion for OCRC may result in tumor cell dissemination into the peripheral circulation and may induce distant metastases. IntroductionAt the time of the initial diagnosis of colorectal cancer, 8-13% of patients have obstructive symptoms (1,2). The treatment of choice for these patients has traditionally been emergency surgery. However, most studies have found that the morbidity and mortality rates are higher for emergency colorectal surgery than for elective surgery, and a temporary colostomy, which decreases patients' quality of life, is needed in many patients, which, in 10-40% of cases, becomes permanent (3). The purpose of stenting as a bridge to surgery (BTS) is to relieve the acute situation without emergency surgery, allowing elective surgery to be performed and thus improve surgical outcomes, determine the correct tumor stage, detect synchronous lesions, stabilize comorbidities, and carry out laparoscopic surgery. Immediate results in higher surgical risk patients who were stented were better for primary anastomosis, permanent stoma, wound infection, and overall morbidity, resulting in greater benefit. However, stent insertion was recently reported to have a high risk of perforation, re-obstruction, or stent migration (4,5). Moreover, perforations can lead to peritoneal dissemination. Sabbagh et al (6) reported worse overall survival and higher 5-year cancer-specific mortality of left-sided obstructive colorectal cancer (OCRC) patients with self-expanding metallic stent (SEMS) insertion than emergency surgery due to perforation induced by stent insertion. Furthermore, SEMS insertion as a BTS is ...

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Section: Impact Of Endoscopic Stent Insertion On Detection Of Viable mentioning

confidence: 99%

Section: Detection Of Ctcs In the Blood Samples Of Ocrcmentioning

confidence: 99%

Impact of endoscopic stent insertion on detection of viable circulating tumor cells from obstructive colorectal cancer

et al. 2017

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“…To our knowledge, the only existing functional test that has been used on hundreds of patients with different tumor types is EPISPOT, which allows the detection of viable clinically relevant CTCs enriched by leukocyte depletion (23,24 ). This technology enables the detection of EpCAM ϩ and EpCAM Ϫ CTCs.…”

Section: Detection Of Viable Ctcsmentioning

confidence: 99%

Functional Studies on Viable Circulating Tumor Cells

Pantel

Alix‐Panabières

2016

Clinical Chemistry

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BACKGROUND Research on circulating tumor cells (CTCs) as new biomarkers has received great attention over the past decade. In particular, the capture and analysis of CTCs as “liquid biopsies” provides the possibility to avoid invasive tissue biopsies, with obvious implications in cancer diagnostics. CONTENT The focus of this review is to describe and discuss how functional studies on viable CTCs can enlarge the spectrum of applications of liquid biopsies, with emphasis on breast, prostate, colon, and lung cancer as the major tumor entities in industrialized countries. The low number of CTCs in the peripheral blood of most cancer patients makes challenging the in vitro culture of CTCs. Epithelial tumor cells are difficult to culture, even when starting with millions of tumor cells. Recently, several groups have achieved important advances in the in vitro and in vivo expansion of CTCs from cancer patients at very advanced stages with higher amounts of CTCs. Here, we present current technologies to enrich and detect viable human CTCs, including positive and negative enrichment strategies that are based on antigen expression and physical properties of CTCs. We also discuss published data about functional studies on CTCs that use in vitro and in vivo models. SUMMARY Functional analyses on CTCs offer the possibility to identify the biological properties of metastatic cells, including the identification of metastasis-initiating cells. Moreover, CTC-derived cell lines and xenografts might reveal new therapeutic targets and can be used for drug screening.

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“…They have a very short life in blood (few hours) and get cleared by spontaneous disintegration or extravasation into liver, bone marrow, and spleen. [7] Advances in the molecular tests such as targeted sequencing, in-situ hybridization, polymerase chain reaction (PCR), single nucleotide polymorphisms, targeted plasma resequencing (TamSeq), and whole genome/whole exome sequencing (WGS/WES) when applied to ctDNA can provide clinicians with a wealth of useful molecular information.…”

Section: Introductionmentioning

confidence: 99%

Liquid biopsy, an innovation of modern medicine

Gujree

Singh

2017

Int J Mol Immuno Oncol.

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<div id="nav"><div id="og_head"><div id="og_q"><div class="og_h"><div class="og_m">Biopsies have been the best tool in the hands of the clinicians for the appropriate management of malignancies including diagnosis, prognosis and reassessment. They are and have been the gold standard in their diagnosis. Due to certain unavoidable limitations of needle biopsy, there is emergence of a new biomarker in oncology called LIQUID BIOPSY: Analysis of circulating tumour cells (CTC) and circulating free tumor DNA (ctDNA) from the peripheral blood. As this technology evolves it may prove to be a critical component of personalized medicine in this modern era of diagnostics. Liquid biopsy has a great potential in cancer diagnosis, monitoring and predicting survival. Nevertheless false positive results exist. The major challenge with this technology is assay sensitivity and specificity. This mini-review tries to fill the gap between reality and our hope of this “liquid biopsy”.</div></div></div></div></div>

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Capture of Viable Circulating Tumor Cells in the Liver of Colorectal Cancer Patients

Abstract: BACKGROUND:The incidence and number of circulating tumor cells (CTCs) in the peripheral blood of colorectal cancer patients are lower than in other cancer types, which may point to a particular biology of colorectal cancer affecting CTC detection.

Cited by 199 publications

References 39 publications

Impact of endoscopic stent insertion on detection of viable circulating tumor cells from obstructive colorectal cancer

Impact of endoscopic stent insertion on detection of viable circulating tumor cells from obstructive colorectal cancer

Functional Studies on Viable Circulating Tumor Cells

Liquid biopsy, an innovation of modern medicine

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