Capturing instructive cues of tissue microenvironment by silica bioreplication

Tang, Sze Wing; Yuen, Wai Shan; Kaur, Ishdeep; Pang, S. W.; Voelcker, Nicolas H.; Lam, Yun Wah

doi:10.1016/j.actbio.2019.11.033

Cited by 11 publications

(14 citation statements)

References 81 publications

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“…The data showed that the MSCs cultured on the substrate formed spheroids and expressed neurosphere markers (Fig. 6d) [56]. More recently, Ghazali et al choose a similar topography technique on adiposederived mesenchymal stem cells (ADSC) [57].…”

Section: Topography-guided Mesenchymal Stem Cell Lineage Reprogrammingmentioning

confidence: 98%

“…Since the piezoelectric material generated an electric potential by converting mechanical forces such as cell movement and traction, rat bone marrow-derived MSCs were directly seeded onto the substrates without further electrical stimulation. Based on the data collected from the study, when 200 [55] published by American Chemical Society 2019, reproduced with permission from [56] published by Elsevier 2020) ◂ and 500 nm patterned polyvinyl chloride (PVC) was used as non-electrical control, nanotopography was sufficient to promote the cellular adhesion of the stem cells. However, only the combination with PVDF could greatly enhance the neuron-like differentiation of the MSCs at the 200 nm pattern.…”

Section: Topography-guided Mesenchymal Stem Cell Lineage Reprogrammingmentioning

confidence: 99%

“…Tang et al used silica BioReplication (SBR) as a method to replicate the topography of multiple mammalian tissues [56]. After paraformaldehyde fixation of animal spinal cord tissue, the samples were incubated in a tetramethyl orthosilicate solution for 24 h before removing the organic residues by calcination (Fig.…”

Section: Topography-guided Mesenchymal Stem Cell Lineage Reprogrammingmentioning

confidence: 99%

See 2 more Smart Citations

Recent Developments in Surface Topography-Modulated Neurogenesis

Amri¹,

Kim

Lee

2021

BioChip J

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Repairing a nervous system damaged following an injury or as a consequence of neurodegeneration is still a challenging task. Yet, topography-based tissue engineering of neurons from different cell sources has demonstrated promising potential in the field. Substrate patterning through a range of methods including photolithography, nanofibers electrospinning, or microimprinting can not only impact the maturity of these vital neural cells but can also orient the differentiation of neural stem cells, a more prolific source of neurons. Furthermore, it has been shown that topography can guide the trans-differentiation of mesenchymal stem cells, a readily accessible cell source, towards a neuronal fate. Thus, in this review, we will cover the most recent methods used in topography-based neural tissue engineering.

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Section: Topography-guided Mesenchymal Stem Cell Lineage Reprogrammingmentioning

confidence: 98%

Section: Topography-guided Mesenchymal Stem Cell Lineage Reprogrammingmentioning

confidence: 99%

Section: Topography-guided Mesenchymal Stem Cell Lineage Reprogrammingmentioning

confidence: 99%

See 1 more Smart Citation

Recent Developments in Surface Topography-Modulated Neurogenesis

Amri¹,

Kim

Lee

2021

BioChip J

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“…Tang et al exploited this method to firstly demonstrate that the precisely replicated tissue topography harbored sufficient information to direct the fate of human MSCs without the need of exogenous factors such as soluble growth factors or immobilized ECM molecules. They suggested as the tissue microenvironment captured by SBR profoundly affected MSC biology, and that the topographical cues were sufficient in initiating and directing differentiation of MSC, despite the absence of any biochemical cues [104].…”

Section: Topographical Cuesmentioning

confidence: 99%

Extracellular Matrix-Derived Hydrogels as Biomaterial for Different Skeletal Muscle Tissue Replacements

et al. 2020

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Recently, skeletal muscle represents a complex and challenging tissue to be generated in vitro for tissue engineering purposes. Several attempts have been pursued to develop hydrogels with different formulations resembling in vitro the characteristics of skeletal muscle tissue in vivo. This review article describes how different types of cell-laden hydrogels recapitulate the multiple interactions occurring between extracellular matrix (ECM) and muscle cells. A special attention is focused on the biochemical cues that affect myocytes morphology, adhesion, proliferation, and phenotype maintenance, underlining the importance of topographical cues exerted on the hydrogels to guide cellular orientation and facilitate myogenic differentiation and maturation. Moreover, we highlight the crucial role of 3D printing and bioreactors as useful platforms to finely control spatial deposition of cells into ECM based hydrogels and provide the skeletal muscle native-like tissue microenvironment, respectively.

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“…96 Using silica bioreplication, a technique originally developed for sample preparation in electron microscopy, 97 Tang et al has recently produced silica replicas of tendon and spinal cord with nanoscale spatial accuracy and showed that MSC cultured on these replicas differentiated into tenocyteand neurosphere-like cells, respectively. 98…”

Section: Replicating the Tmementioning

confidence: 99%

Deconstructing, Replicating, and Engineering Tissue Microenvironment for Stem Cell Differentiation

Tang

Tong

Pang

et al. 2020

Tissue Engineering Part B: Reviews

Self Cite

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One of the most crucial components of regenerative medicine is the controlled differentiation of embryonic or adult stem cells into the desired cell lineage. Although most of the reported protocols of stem cell differentiation involve the use of soluble growth factors, it is increasingly evident that stem cells also undergo differentiation when cultured in the appropriate microenvironment. When cultured in decellularized tissues, for instance, stem cells can recapitulate the morphogenesis and functional specialization of differentiated cell types with speed and efficiency that often surpass the traditional growth factor-driven protocols. This suggests that the tissue microenvironment (TME) provides stem cells with a holistic ''instructive niche'' that harbors signals for cellular reprogramming. The translation of this into medical applications requires the decoding of these signals, but this has been hampered by the complexity of TME. This problem is often addressed by a reductionist approach, in which cells are exposed to substrates decorated with simple, empirically designed geometries, textures, and chemical compositions (''bottom-up'' approach). Although these studies are invaluable in revealing the basic principles of mechanotransduction mechanisms, their physiological relevance is often uncertain. This review examines the recent progress of an alternative, ''top-down'' approach, in which the TME is treated as a holistic biological entity. This approach is made possible by recent advances in systems biology and fabrication technologies that enable the isolation, characterization, and reconstitution of TME. It is hoped that these new techniques will elucidate the nature of niche signals so that they can be extracted, replicated, and controlled. This review summarizes these emerging techniques and how the data they generated are changing our view on TME.

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Capturing instructive cues of tissue microenvironment by silica bioreplication

Cited by 11 publications

References 81 publications

Recent Developments in Surface Topography-Modulated Neurogenesis

Recent Developments in Surface Topography-Modulated Neurogenesis

Extracellular Matrix-Derived Hydrogels as Biomaterial for Different Skeletal Muscle Tissue Replacements

Deconstructing, Replicating, and Engineering Tissue Microenvironment for Stem Cell Differentiation

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