Capturing pore-forming intermediates of MACPF and binary toxin assemblies by cryoEM

Couves, Emma C.; Bubeck, Doryen

doi:10.1016/j.sbi.2022.102401

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…The MACPF protein has the ability to create pores in cellular membranes [5]. Its structure is closely associated with cholesterol-dependent cytolysins and the complement system, which includes proteins such as C6, C7, C8α, C8β, and C9 [6][7][8][9]. In recent times, the resolution of several MACPF protein structures has revealed that the MACPF domain of complement proteins undergoes significant structural rearrangements during the formation of pores.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the Membrane Attack Complex and Perforin Genes and Their Expression Pattern under Stress in the Solanaceae

Ma,

Guo,

Liu

et al. 2023

IJMS

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The Membrane Attack Complex and Perforin (MACPF) proteins play a crucial role in plant development and adaptation to environmental stresses. Heretofore, few MACPF genes have been functionally identified, leaving gaps in our understanding of MACPF genes in other plants, particularly in the Solanaceae family, which includes economically and culturally significant species, such as tomato, potato, and pepper. In this study, we have identified 26 MACPF genes in three Solanaceae species and in the water lily, which serves as the base group for angiosperms. Phylogenetic analysis indicates that angiosperm MACPF genes could be categorized into three distinct groups, with another moss and spikemoss lineage-specific group, which is further supported by the examination of gene structures and domain or motif organizations. Through inter-genome collinearity analysis, it is determined that there are 12 orthologous SolMACPF gene pairs. The expansion of SolMACPF genes is primarily attributed to dispersed duplications, with purifying selection identified as the principal driving force in their evolutionary process, as indicated by the ω values. Furthermore, the analysis of expression patterns revealed that Solanaceae genes are preferentially expressed in reproductive tissues and regulated by various environmental stimuli, particularly induced by submergence. Taken together, these findings offer valuable insights into and a fresh perspective on the evolution and function of SolMACPF genes, thereby establishing a foundation for further investigations into their phenotypic and functional characteristics.

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