Capturing volumetric dynamics at high speed in the brain by confocal light field microscopy

Abstract

Neural network performs complex computations through coordinating collective neural dynamics that are fast and in three-dimensions. Meanwhile, its proper function relies on its 3D supporting environment, including the highly dynamic vascular system that drives energy and material flow.Better understanding of these processes requires methods to capture fast volumetric dynamics in thick tissue. This becomes challenging due to the trade-off between speed and optical sectioning capability in conventional imaging techniques. Here we present a new imaging method, confocal light field microscopy, to enable fast volumetric imaging deep into brain. We demonstrated the power of this method by recording whole brain calcium transients in freely swimming larval zebrafish and observed behaviorally correlated activities on single neurons during its prey capture. Furthermore, we captured neural activities and circulating blood cells over a volume ⌀ 800 μm x 150 μm at 70 Hz and up to 600 μm deep in the mice brain.

Learning to Reconstruct Confocal Microscopy Stacks from Single Light Field Images

“…Also, a mathematical model was developed and later used to recover rigid body full-field displacement measurements. Zhang et al [11] proposed the confocal LFM. Taking the idea from confocal microscopy, their system blocks the out of focus light coming from the specimen, achieving high axial resolution at the expense of increased exposure time.…”

“…This is achieved by placing a micro-lens array (MLA) in the optical path and by using an algorithm (e.g.deconvolution) to reconstruct the observed volume from the light field (LF) image. It enables advanced computational applications, such as in vivo calcium imaging of neural activity, in immobilized [5][6][7][8][9][10][11] and freely moving animals [12][13][14][15]. Also, it has been used to image mouse brain tissue, which has high scattering up to 380µm in depth [7].…”

Lutein Attenuates Cyclosporin-induced Testicular Impairment in Male Rats through Modulation of Androgenic Hormones and Enzymes

“…Lutein is a plant-derived xanthophyll that naturally occurs in fruits and vegetables such as spinach, lima beans and orange (Sommerburg et al, 1998). Lutein is a potent antioxidant (O'Nell et al, 2001;Johnson, 2002;He et al, 2011;Kim et al, 2012;Du et al, 2015;Mammadov et al, 2019) with various biological and protective effects including hepatoprotective , anticancer (Zhang et al, 2018), neuroprotective (Binawade et al, 2013), anti-diabetics (Neelam et al, 2017), reproductiveprotective , and anti-inflammatory (Chung et al, 2017) properties. Moreover, the antioxidant activity of lutein has been linked to reduced rate of accelerated aging (He et al, 2011;Li et al, 2010).…”