Carabrol suppresses LPS-induced nitric oxide synthase expression by inactivation of p38 and JNK via inhibition of I-κBα degradation in RAW 264.7 cells

Lee, Hwa Jin; Lim, Hyo Jin; Lee, Da Yeon; Jung, Hyeyoun; Kim, Miran; Moon, Dong-Cheul; Kim, Keun Il; Ryu, Jae‐Ha

doi:10.1016/j.bbrc.2009.12.073

Cited by 32 publications

(19 citation statements)

References 27 publications

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“…Despite its protective functions, the excessive production of NO contributes to the pathogenesis of inflammation, cellular injury and tissue damages in a lung [3,12,17,32]. We investigated the inhibitory effects of HPOX nanoparticles on NO production during the inflammatory response of LPS-stimulated cells.…”

Section: Discussionmentioning

confidence: 99%

“…It was also reported that human asthmatics have the higher level of NO and increased expression of iNOS (inducible nitric oxide synthases) in the airway of epithelium than non-asthmatic humans [11]. Therefore, ROS have great potential as a diagnostic and therapeutic biomarker of various inflammatory responses and there is increasing interest for the development of strategies to reduce oxidative stress by ROS [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…HBA is one of phenolic compounds and plays a protective role against oxidative damage-related diseases such as ischemic brain injury and coronary heart diseases [5,12,13]. It is also a powerful scavenger of free radicals such as superoxide and hydroxyl radical and exerts antioxidant activities [14].…”

Section: Introductionmentioning

confidence: 99%

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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“…31 In brief, RAW 264.7 cells (8 · 10 5 cells per 60-mm-diameter dish) were treated with 1 lg/mL LPS in the presence or absence of test compounds. Following 20-h treatment, cells were harvested and gently lysed with cell lysis buffer (Cell Signaling Technologies, Beverly, MA, USA).…”

Section: Western Blot Analysismentioning

confidence: 99%

Anti-Inflammatory Activity of Sulfur-Containing Compounds from Garlic

Lee

Lim

et al. 2012

Journal of Medicinal Food

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We identified four anti-inflammatory sulfur-containing compounds from garlic, and their chemical structures were identified as Z-and E-ajoene and oxidized sulfonyl derivatives of ajoene. The sulfur compounds inhibited the production of nitric oxide (NO) and prostaglandin E2 (PGE2) and the expression of the pro-inflammatory cytokines tumor necrosis factora, interleukin-1b, and interleukin-6 in lipopolysaccharide (LPS)-activated macrophages. Western blotting and reverse transcription-polymerase chain reaction analysis demonstrated that these sulfur compounds attenuated the LPS-induced expression of the inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins and mRNA. Moreover, these sulfurcontaining compounds suppressed the nuclear factor-jB (NF-jB) transcriptional activity and the degradation of inhibitoryjBa in LPS-activated macrophages. Furthermore, we observed that they markedly inhibited the LPS-induced phosphorylations of p38 mitogen-activated protein kinases and extracellular signal-regulated kinases (ERK) at 20 lM. These data demonstrate that the sulfur compounds from garlic, (Z, E)-ajoene and their sulfonyl analogs, can suppress the LPS-induced production of NO/ PGE2 and the expression of iNOS/COX-2 genes by inhibiting the NF-jB activation and the phosphorylations of p38 and ERK. Taken together, these data show that Z-and E-ajoene and their sulfonyl analogs from garlic might have anti-inflammatory therapeutic potential.

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“…Many evidences reported that MAPKs signaling cascades might be differentially involved in the macrophage response to anti-inflammatory compounds Lee et al, 2010;Park et al, 2008;Zhou et al, 2008). In order to explore the mechanisms underlying the inhibitory effect of polyphenol-rich fractions on NO production, phosphorylation levels of p38 MAPK, JNK 1/2, ERK 1/2 and Akt were analyzed by Western blot in LPS-stimulated Raw 264.7 macrophages.…”

Section: Page 25 Of 30mentioning

confidence: 99%

Cymbopogon citratus as source of new and safe anti-inflammatory drugs: Bio-guided assay using lipopolysaccharide-stimulated macrophages

Francisco

Figueirinha

Neves

et al. 2011

Journal of Ethnopharmacology

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Please cite this article as: Francisco, V., Figueirinha, A., Neves, B.M., García-Rodríguez, C., Lopes, M.C., Cruz, M.T., Batista, M.T., Cymbopogon citratus as source of new and safe anti-inflammatory drugs: bio-guided assay using lipopolysaccharide-stimulated macrophages, Journal of Ethnopharmacology (2010Ethnopharmacology ( ), doi:10.1016Ethnopharmacology ( /j.jep.2010 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 30A c c e p t e d M a n u s c r i p t 1 Graphical abstractIn this report it was demonstrated that a lipid-and essential oil-free infusion of Cymbopogon citratus leaves (Cy), as well its polyphenols, have anti-inflammatory properties through inhibition of proinflammatory signaling pathways and nitric oxide production in lipopolysaccharide-stimulated macrophages. These evidences support the use of Cymbopogon citratus in traditional medicine and indicate that it could be a natural source of new and safe anti-inflammatory drugs. TitleCymbopogon citratus as source of new and safe anti-inflammatory drugs: bio-guided assay using lipopolysaccharide-stimulated macrophages Aim of the study: The aim of this study is to explore the anti-inflammatory properties of Cymbopogon citratus leaves and their polyphenol-rich fractions (PFs), as well its mechanism of action in murine macrophages. Materials and Methods:A lipid-and essential oil-free infusion of Cy leaves was prepared (Cy extract) and fractionated by column chromatography. Anti-inflammatory properties of Cy extract (1.115 mg/ml) and its PFs, namely phenolic acids (530 µg/ml), flavonoids (97.5 µg/ml) and tannins (78 µg/ml), were investigated using lipopolysaccharide (LPS)-stimulated Raw 264.7 macrophages as in vitro model. As inflammatory parameters, nitric oxide (NO) production was evaluated by Griess reaction, as well as effects on cyclooxygenase (COX-2), inducible NO synthase (iNOS) expression and on intracellular signaling pathways activation, which were analyzed by Western blot using specific antibodies. Page 3 of 30A c c e p t e d M a n u s c r i p t Conclusions: Our data provide evidence that support the usage of Cymbopogon citratus leaves extract in traditional medicine, and suggest that Cy, in particular its polyphenolic compounds, could constitute a natural source of a new and safe anti-inflammatory drugs.

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Carabrol suppresses LPS-induced nitric oxide synthase expression by inactivation of p38 and JNK via inhibition of I-κBα degradation in RAW 264.7 cells

Cited by 32 publications

References 27 publications

Reduction of oxidative stress by p-hydroxybenzyl alcohol-containing biodegradable polyoxalate nanoparticulate antioxidant

Reduction of oxidative stress by p-hydroxybenzyl alcohol-containing biodegradable polyoxalate nanoparticulate antioxidant

Anti-Inflammatory Activity of Sulfur-Containing Compounds from Garlic

Cymbopogon citratus as source of new and safe anti-inflammatory drugs: Bio-guided assay using lipopolysaccharide-stimulated macrophages

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