Caractérisation d'anticorps monoclonaux murins dirigés contre les érythrocytes fœtaux

Blanchard, Dominique; Bernard, Daniel; Loirat, M.J.; Frioux, Y.; Guimbretière, J; Guimbretière, L.

doi:10.1016/s1140-4639(05)80102-9

Cited by 10 publications

(3 citation statements)

References 15 publications

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“…The LPS from strain J223 was serotyped in ELISA using procedures that have been previously described (9,11). For serotyping of H. pylori J223, the following mAbs were employed (11): CB-10 and 54.1F6A (both anti-Le , 4D2 (anti-H type 1), 3-3A (anti-blood group A), and NAM61-1A2 (anti-i antigen) (24). H. pylori J223 LPS was subjected to SDS-polyacrylamide gel electrophoresis, silver-stained, and immunoblotted as described previously (11).…”

Section: Table II Interpretation Of the Ions From The Fab-ms Spectrummentioning

confidence: 99%

Simultaneous Expression of Type 1 and Type 2 Lewis Blood Group Antigens by Helicobacter pyloriLipopolysaccharides

Monteiro¹,

Chan²,

Rasko³

et al. 1998

Journal of Biological Chemistry

156

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Section: Table II Interpretation Of the Ions From The Fab-ms Spectrummentioning

confidence: 99%

Simultaneous Expression of Type 1 and Type 2 Lewis Blood Group Antigens by Helicobacter pyloriLipopolysaccharides

Monteiro¹,

Chan²,

Rasko³

et al. 1998

Journal of Biological Chemistry

156

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“…The switch to I‐antigen, a branched form of the linear i‐antigen, happens after birth (16). Anti‐i monoclonal antibody, previously described (6, 17), was used either directly conjugated to FITC or to alkaline phosphatase (AP) or by an indirect amplification procedure using unconjugated anti‐i followed by incubation with goat anti‐mouse immunoglobulin conjugated to an AP labeled polymer (Dako EnVision System, Copenhagen, Denmark) (18). APase activity was detected using three different chromogens (Fast Red, 5‐bromo‐4 chloro‐3‐indolyl phosphate/nitro blue tetrazolium/ iodonitrotetrazolium violet (BCIP/NBT/INT), or 5‐bromo‐4chloro‐3‐indolyl phosphate‐nitro blue tetrazolium (BCIP/NBT) to compare image intensities and qualities appropriate to our study.…”

Section: Methodsmentioning

confidence: 99%

Detection of chromosomal aneuploidies in fetal cells isolated from maternal blood using single‐chromosome dual‐probe FISH analysis

Calabrese

Baldi²,

Fantasia

et al. 2011

Clinical Genetics

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Detection of chromosomal aneuploidies using fetal cells isolated from maternal blood, for prenatal non-invasive genetic investigation, has been a long-sought goal of clinical genetics to replace amniocentesis and chorionic villous sampling to avoid any risk to the fetus. The purpose of this study was to develop a sensitive and specific new assay for diagnosing aneuploidy with circulating fetal cells isolated from maternal blood as previously reported using two novel approaches: (i) simultaneous immunocytochemistry (ICC) evaluation using a monoclonal antibody for i-antigen, followed by fluorescence in situ hybridization (FISH); (ii) dual-probe FISH analysis of interphase nuclei using two differently labeled probes, specific for different loci of chromosomes 21 and 18; in addition, short tandem repeats (STR) analysis on single cells isolated by micromanipulation was applied to confirm the presence of fetal cells in the cell sample enriched from maternal blood. Blood samples were obtained from women carrying trisomic fetuses, and from non-pregnant women and men as controls. Using ICC-FISH approach, a large heterogeneity in immunostaining pattern was observed, which is a source of very subjective signal interpretation. Differently, dual-probe FISH analysis provided for a correct diagnosis of all pregnancies: the mean percentage of trisomic cells was 0.5% (range, 0.36-0.76%), while the mean percentage of trisomic cells in the control group (normal pregnancies or non-pregnant women) was ≤0.20%. The application of the dual-probe FISH protocol on fetal cells isolated from maternal blood enables accurate molecular detection of fetal aneuploidy, thus providing a foundation for development of non-invasive prenatal diagnostic testing.

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“…Anti-i monoclonal antibody used in the present investigation has been previously described in details [22]. Isolated cells were resuspended in a final volume of 500 Al at a concentration of 1 Â 10 6 cells/ml in RPMI 1640 containing 1% BSA and anti-i monoclonal antibody for 2 h. After two washings in RPMI 1640, cells were sedimented on glass slides at 1 g and left drying overnight at RT.…”

Section: Immunostaining With Anti-i Moabmentioning

confidence: 99%

The use of non-physiological conditions to isolate fetal cells from maternal blood

Sitar

Brambati

Baldi

et al. 2005

Experimental Cell Research

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Caractérisation d'anticorps monoclonaux murins dirigés contre les érythrocytes fœtaux

Cited by 10 publications

References 15 publications

Simultaneous Expression of Type 1 and Type 2 Lewis Blood Group Antigens by Helicobacter pyloriLipopolysaccharides

Simultaneous Expression of Type 1 and Type 2 Lewis Blood Group Antigens by Helicobacter pyloriLipopolysaccharides

Detection of chromosomal aneuploidies in fetal cells isolated from maternal blood using single‐chromosome dual‐probe FISH analysis

The use of non-physiological conditions to isolate fetal cells from maternal blood

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