Características do sêmen de carneiros Dorper, Santa Inês e sem padrão racial definido, pré e pós-congelação, nos períodos chuvoso e seco

Sobrinho, João Mendes Frazão; Branco, Marlon de Araújo Castelo; Júnior, Antônio Sousa; Nascimento, I. M. R. do; Mota, Luiz Harliton Cavalcante Monteiro; Carvalho, Y. N. T.; Ferreira, Siluana Benvindo; Costa, Deyse Nayra Mascarenhas; Junior, Fenerick; Souza, José Adalmir Torres de

doi:10.1590/1678-6465

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Fresh semen values were similar to those observed in experiments that used Santa Inês breed in the same location (Maia et al, 2011;Frazão Sobrinho et al, 2014).…”

Section: Discussionsupporting

confidence: 86%

Effect of adenosine concentration on quality of cooled ram semen

et al. 2019

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This study analyzed the adenosine concentration on quality of ram semen in different refrigerated storage times at 5 °C. The design was in blocks with repeatedmeasures factorial design. Five levels of adenosine and four refrigeration times were considered as fixed effects and the animal as random effect (block). Ram semen was diluted with Andromed ® diluent (control) and increasing adenosine levels (0.5, 0.75, 1, and 1.5%). The samples were taken from refrigeration after 4, 8, 12, and 16 h and evaluated for progressive rectilinear sperm motility, sperm vigor, and membrane integrity (supravital test). Data were subjected to analysis of variance with Tukey's test. The regression analysis was conducted to evaluate behavior of adenosine levels. Factor interaction for rectilinear progressive motile variable was verified. There was a quadratic effect of progressive rectilinear motility in function of adenosine levels for each cooled storage time studied, with recommended maximum adenosine concentration of 0.81 and 1.04% at 4 and 8 h, respectively. Adenosine also promoted a protective effect on the membrane integrity. Adenosine added to the diluent increases sperm motility and vigor and protects the sperm membrane integrity.

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“…Fresh semen values were similar to those observed in experiments that used Santa Inês breed in the same location (Maia et al, 2011;Frazão Sobrinho et al, 2014).…”

Section: Discussionsupporting

confidence: 86%

Effect of adenosine concentration on quality of cooled ram semen

et al. 2019

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“…Lambs vaccinated in the current study, regardless of dose, showed a 23.28% increase in the number of abnormal spermatozoa. Frazão et al (2014) obtained 24.30% ± 2.9% total sperm pathologies, similar to those of the control group in the current study. CBRA (2013) recommends that this value be lower than 20%.…”

Section: Seminal Fluid Production and Qualitysupporting

confidence: 88%

Reproductive parameters of lambs immunocastrated with anti-GnRH vaccine

et al. 2021

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The objective of this study was to evaluate the testicular biometric, seminal, and plasma testosterone levels in lambs subjected to an anti-GnRH vaccine as a method of castration. Thirty entire, crossbred Santa Inês male lambs were randomly distributed into three treatment (T): T1 was the control group, with the administration of 1 mL of saline solution subcutaneously (SC); 1.0 and 0.5 mL of an anti-GnRH vaccine were administered SC in T2 and T3, respectively. Testicular biometric variables, physical and morphological variables of semen, and plasma testosterone concentrations were evaluated. At D60, there was a reduction in testicular length, width, thickness, and scrotal circumference of the immunocastrated animals regardless of the vaccine dose used (P < 0.05). A reduction in semen physical variables at both dosages (P < 0.05) was observed, with azoospermia, in 80% and 70% of animals in the T2 and T3 groups, respectively. At D60, the immunocastrated animals also showed an increase in spermatozoa defects (P < 0.05), whereas plasma testosterone concentration decreased (P < 0.05). Immunocastration of lambs using the Bopriva vaccine at doses of 1.0 and 0.5 mL is efficient in inducing azoospermia in up to 80% of animals, although two doses in a 30-day interval are necessary for it to be an effective and safe method. Efficacy was demonstrated through a reduction in serum testosterone levels, testicular biometry, and seminal fluid analysis. Considering the efficacy of both doses in this study, we recommend using the lower dose (0.5 mL), which will allow for a 50% reduction in vaccine costs.

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