Caracterização clínica e imunoistoquímica dos adenomas clinicamente não-funcionantes de hipófise: clinical features and immunohistochemistry

Ferreira, Jane Eyre Alves; Mello, Paulo Andrade de; Magalhães, Albino Verçosa de; Botelho, Carlos Henrique Aguiar; Naves, Luciana Ansaneli; Nosé, Vânia; Schmitt, Fernando

doi:10.1590/s0004-282x2005000600029

Cited by 15 publications

(7 citation statements)

References 23 publications

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“…However, its use in pituitary tumors is still questionable due to heterogeneity of studies, with lack of technical standardization, and the use of semiquantitative methods [35, 36]. In our series, the Ki-67 cutoff was established when positivity was found in ≥ 10% (2+) of cells, according to local standards [14, 15]. Some authors suggested that high immunostaining of Ki-67 could predict aggressive tumor behavior and distinguish invasive from noninvasive adenomas with high sensitivity and specificity [35].…”

Section: Discussionmentioning

confidence: 99%

“…An immunohistochemistry analysis of the samples was previously performed at the Pathology Unit of the University Hospital of Brasilia, and the preliminary results were published [14, 15]. …”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemical evaluation included hormonal and proliferation markers. Detailed information on the antibodies, clones, and dilutions used in the procedure had been already published [14, 15] (Table 1). Reactions were developed with diaminobenzidine (DAB) and counterstained with hematoxylin.…”

Section: Methodsmentioning

confidence: 99%

“…Proliferation markers Ki-67, p53, and c-erbB2 were obtained in a semiquantitative method. The results were considered positive for cases where p53 ≥ 3+ (immunoexpression from 25 to 50% of cells), Ki-67 ≥ 2+ (immunoexpression from 10 to 25% of cells), and c-erbB2 ≥ 2+ (positivity in more than 10% of cells) according to local protocols [14, 15] (Appendix B). …”

Section: Methodsmentioning

confidence: 99%

“…The immunostaining was performed in a semiquantitative method, based on a visual scale. The used antibodies, clones, and respective dilutions were previously validated and published by our research group [14, 15]. …”

Section: Methodsmentioning

confidence: 99%

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Prognostic Value of Invasion, Markers of Proliferation, and Classification of Giant Pituitary Tumors, in a Georeferred Cohort in Brazil of 50 Patients, with a Long-Term Postoperative Follow-Up

Zakir

Casulari

Rosa

et al. 2016

International Journal of Endocrinology

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Although some pituitary adenomas may have an aggressive behavior, the vast majority are benign. There are still controversies about predictive factors regarding the biological behavior of these particular tumors. This study evaluated potential markers of invasion and proliferation compared to current classification patterns and epidemiogeographical parameters. The study included 50 patients, operated on for tumors greater than 30 mm, with a mean postoperative follow-up of 15.2 ± 4.8 years. Pituitary magnetic resonance was used to evaluate regrowth, invasion, and extension to adjacent tissue. Three tissue biomarkers were analyzed: p53, Ki-67, and c-erbB2. Tumors were classified according to a combination of histological and radiological features, ranging from noninvasive and nonproliferative (grade 1A) to invasive-proliferative (grade 2B). Tumors grades 2A and 2B represented 42% and 52%, respectively. Ki-67 (p = 0.23) and c-erbB2 (p = 0.71) had no significant relation to tumor progression status. P53 (p = 0.003), parasellar invasion (p = 0.03), and classification, grade 2B (p = 0.01), were associated with worse clinical outcome. Parasellar invasion prevails as strong predictive factor of tumor recurrence. Severe suprasellar extension should be considered as invasion parameter and could impact prognosis. No environmental factors or geographical cluster were associated with tumor behavior.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Prognostic Value of Invasion, Markers of Proliferation, and Classification of Giant Pituitary Tumors, in a Georeferred Cohort in Brazil of 50 Patients, with a Long-Term Postoperative Follow-Up

Zakir

Casulari

Rosa

et al. 2016

International Journal of Endocrinology

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Elevated risk of recurrence and retreatment for silent pituitary adenomas

Gupta,

Hoffman,

Mehta

et al. 2024

Pituitary

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Vasculogenic Mimicry in Clinically Non-functioning Pituitary Adenomas: a Histologic Study

Michele¹,

Rotondo²,

Kovács³

et al. 2017

Pathol. Oncol. Res.

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The term "vasculogenic mimicry" (VM) refers to the phenomenon in which vascular-like channels, which are not lined by endothelial cells, are formed in tumors. Since its discovery in 1999, it has been observed in several tumor types and is proposed to provide blood perfusion to tumors in absence of co-apted or neo-angiogenic blood vessels. Pituitary tumors are generally slow growing, benign adenomas which are less vascularized than the normal pituitary gland. To date, VM in pituitary adenomas has not been described. In this histological study, we assessed the presence of VM in a series of surgically resected clinically non-functioning pituitary adenomas (NFPAs) using CD34 and Periodic Acid-Schiff (PAS) double staining. To identify VM, slides were assessed for the presence of CD34-negative and PAS-positive channels indicating that they were not lined by endothelial cells. The histological staining pattern suggestive of VM was noted in 22/49 (44.9%) of the specimens studied. VM was observed in both recurring and non-recurring NFPAs. The incidence of VM present varied from case to case and within groups. There was no association between the presence of VM and gender, tumor size, Ki-67 index, recurrence or cavernous sinus invasion. VM was not noted in cases of non-tumorous pituitaries. Our findings suggest the existence of a complementary perfusion system in pituitary adenomas, implying potential clinical implications with respect to response to therapy and clinical course. Further research is warranted to confirm the presence of VM in pituitary adenomas to elucidate its clinical relevance in patients diagnosed with a pituitary adenoma.

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Caracterização clínica e imunoistoquímica dos adenomas clinicamente não-funcionantes de hipófise: clinical features and immunohistochemistry

Cited by 15 publications

References 23 publications

Prognostic Value of Invasion, Markers of Proliferation, and Classification of Giant Pituitary Tumors, in a Georeferred Cohort in Brazil of 50 Patients, with a Long-Term Postoperative Follow-Up

Prognostic Value of Invasion, Markers of Proliferation, and Classification of Giant Pituitary Tumors, in a Georeferred Cohort in Brazil of 50 Patients, with a Long-Term Postoperative Follow-Up

Elevated risk of recurrence and retreatment for silent pituitary adenomas

Vasculogenic Mimicry in Clinically Non-functioning Pituitary Adenomas: a Histologic Study

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