The objective was to evaluate the microbiological quality, as well as the presence of antimicrobial residues in milk sold in the city of Goiânia-GO. Six milk samples were collected at four times. The experiment proceeded in two stages: 1) microbiological analysis of orientation, to verify the microbiological standards, according to the Resolution of the Collegiate Board nº12 and by the method of the American Public Health Association; and 2) detection of the presence of antimicrobial residues, using the disk-diffusion antibiogram method with control of the experiment carried out by inoculating Escherichia coli with the antibiotics ampicillin, cephalothin, penicillin and tetracycline. Negative results were observed for the presence of total coliforms and antimicrobial residues in the investigated samples. It was also observed, a total sensitivity of the microorganism Escherichia coli, against the antibiotics ampicillin, cephalothin and tetracycline, in the control experiment. However, for penicillin there was a change in behavior, and initially the bacteria was sensitive to the drug and, throughout the analysis, it showed resistance to the drug. The results suggest a good sanitary hygienic quality control of the industries that handle the milk samples selected for the present study, revealing the concern with the supply of a quality food product. And, coupled with this, it guarantees consumer health and fluidity in the technological processing of the processed milk.