Caracterização histológica e imuno-histoquímica das lesões de tuberculose em bovinos e de linfadenite granulomatosa em suínos

Andreazza, Daniele; Boos, Gisele Silva; Boabaid, Fabiana M.; Wouters, Angélica Terezinha Barth; Wouters, Flademir; Souza, Suyene Oltramari de; Menegat, Mariana Boscato; Driemeier, David

doi:10.1590/s0100-736x2015000200006

Cited by 9 publications

(9 citation statements)

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“…Commonly, the bovine affected by the disease present progressive weight loss, and in some cases unproductive cough; these signs are associated to the aerial transmission way (Andreazza et al 2015). Sporadically, young bovines present clinical neurological manifestation and granulomatous lesions in the leptomeninges of the encephalon and spinal cord (Oruç 2005, Riet-Correa & Garcia 2007, Del Fava et al 2010.…”

Section: Introductionmentioning

confidence: 99%

Tuberculosis of the central nervous system in cattle in Paraíba, Brazil

et al. 2018

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This paper describes six cases of tuberculosis in the central nervous system (CNS) of cattle in the state of Paraíba in northeastern Brazil. We reviewed the autopsy reports of 851 bovine necropsies performed from 2003 to 2016. Seventy-three (8.6%) cattle were diagnosed with tuberculosis and six showed lesions in the CNS. Three cases affected cattle up to two-year-old and other three affected adults. Three cattle presented exclusively nervous signs, two had respiratory signs and weight loss and one did not present any clinical signs. At necropsy, five cattle had thickening of the leptomeninges of the cerebellum, pons, obex, spinal cord and cortex, mainly, in the region near the brain basilar Willis´ circle. Another animal, presented a single focal lesion in the cerebellum. Microscopically we observed moderate to severe granulomatous meningitis and encephalitis. Five cattle presented lesions in the lungs and mediastinal lymph nodes and three of them had disseminated lesions in other organs. In all cattle acid-fast bacilli were observed in the lesions and marked positive for immunohistochemistry with polyclonal antibody anti-Mycobacterium tuberculosis. It is concluded that bovine tuberculosis of central nervous system occurs sporadically in Paraíba, in cattle of different ages, most of them with disseminate lesions in other organs. The location of the lesions suggests that the agent invaded the brain by hematogenous route through the circle of Willis.

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Section: Introductionmentioning

confidence: 99%

Tuberculosis of the central nervous system in cattle in Paraíba, Brazil

et al. 2018

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“…Although the clinical condition of HCC, bovine leukosis and tuberculosis is similar, the histological appearance between them is quite different. In leukosis, it is common to observe clusters of neoplastic lymphocytes in organs [23], while in bovine tuberculosis it is possible to observe the presence of resistant acid alcohol bacilli in Ziehl-Neelsen staining, indicating the presence of Mycobacterium bovis [3], which was negative in the present report.…”

Section: Discussionmentioning

confidence: 42%

“…Blood samples were collected by jugular venipuncture in Vacutainer siliconized tubes containing 10% EDTA (Vacuette ® ) 3 for measurements of blood count, total plasma protein (TPP), and plasma fibrinogen [11]. The complete blood count revealed hypoproteinemia of 4.2 g/dL (reference range: 7-8.5); hypofibrinogenemia of 200 mg/dL (reference range: 300-700); thrombocytopenia of 186,000/μL (reference range: 200,000 -730,000), and neutrophilia with 4.644/μL (reference range: 600-4000).…”

Section: Casementioning

confidence: 99%

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Clinical - Pathological Aspects of Hepatocellular Carcinoma in Cattle

Mendonça

Costa

Biscarde

et al. 2019

Acta Scientiae. Vet.

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Background:The hepatocellular carcinoma (HCC) is a malignant neoplasm of hepatocytes of rare occurrence in farm animals, with ruminants being the most affected species. This neoplasm is characterized by nonspecific symptoms and it is etiology in animals has not yet been fully elucidated, although aflatoxin has been shown to be a risk factor in the development this neoplasia. Since hepatic tumors in cattle are commonly incidental findings found in postmortem examination, the objective of this paper is to describe the clinical, laboratory and pathological findings in a cow with this neoplasm. Case: A 5-year-old, adult, Girolando cow weighing 350 kg was referred to the Veterinary Hospital of the Federal University of Recôncavo da Bahia, Brazil, with a history of decreased appetite and weakness for one month. The animal was raised in a semi-intensive system, with a corn-based diet, and regular vaccination. Futhermore, was not treated at the farm of origin and three days before being admitted to the hospital, began to present edema of the dewlap. On examination at our center, the cow was in lean, active, with mucupurulent secretion in nostrils; ocular conjunctiva edema; and edema of the dewlap. The cow had neutrophilia, hypofibrinogemia, hypoproteinemia, and trombocytopenia. It also had tachycardia, tense abdomen, engorgement of the vessels of the face and jugular veins, stasis test and bilaterally positive jugular pulse. Although evidence of pain in reticulum was negative in the examination, the initial diagnostic suspicion established was of traumatic reticular pericarditis. The therapeutic protocol instituted was daily monitoring, flunixin meglumine and flofernicol. The examination of rectal palpation revealed in the right flank an irregular-sized parenchymal structure with enlarged and palpacion in pain. Thus, by location and texture, it was suspected that the structure in question was a liver or kidney. However, the dosage of creatinine ruled out renal damage, and persistent hypoproteinemia reinforced the suspicion of hepatic impairment. In view the deterioration of the clinical condition and the suspicion of hepatic impairment, such as hepatic insufficiency, hepatitis or hepatic abscess, we opted for a right exploratory laparotomy. The surgery confirmed the presence of various granulomas throughout the liver, a non-luster organ, bulging edges with erosions and deposition of fibrin on the surface, confirming liver dysfunction. However, the animal died during the surgical procedure, and necropsy was performed, evidencing a focally extensive mass and multiple nodules of various sizes in the liver, lymph nodes and lung. In the microscopy of these nodules, cells similar to hepatocytes were observed, arranged in the form of strands or in agglomerates. Thus, similar to the reports in the literature, the diagnosis of HCC was carried out postmortem by necropsy and histopathology. Discussion: The diagnosis of HCC was based on clinical, laboratory and anatomopathological findings. The hypoproteinemia and persistent hypofib...

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“…Quando a quantidade de bacilos é muito pequena, o local preferencial de pesquisa histopatológica é ao redor dos focos de necrose caseosa, em macrófagos epitelioides e de Langhans. Apenas a morfologia, tanto macro quanto microscópica das lesões da tuberculose, não permite distinguir qual a espécie de Mycobacterium envolvida, uma vez que as lesões induzidas pelas diversas espécies de micobactérias têm semelhanças entre si (Andreazza et al 2015).…”

Section: Discussionunclassified

Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)

Ribeiro¹,

Souza

Casagrande

et al. 2017

Pesq. Vet. Bras.

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RESUMO: A tuberculose é uma enfermidade infectocontagiosa, debilitante, causada por bacilos álcool-ácido resistentes (BAAR), pertencentes ao complexo Mycobacterium tuberculosis (CMT). As micobacterioses têm importância em Medicina Veterinária devido ao seu potencial zoonótico e sua distribuição mundial, afetando todas as classes de vertebrados. Em animais selvagens as micobacterioses têm sido um problema relatado principalmente em cativeiro. Contudo, há relatos de sua ocorrência também em animais de vida livre, colocando em risco e dificultando os programas de erradicação da tuberculose em animais de produção. O diagnóstico nas espécies selvagens em geral é post mortem, uma vez que o teste de tuberculina não está padronizado para essas espécies, assim como não é confiável para triagem. São consideradas para o diagnóstico lesões de necropsia, observação microscópica de BAAR na coloração de Ziehl-Neelsen (ZN) e, principalmente, isolamento e identificação do agente. No entanto, apenas os achados morfológicos macro e microscópicos não permitem distinguir a espécie de Mycobacterium envolvida. A técnica de imuno-histoquímica (IHQ) com anticorpo policlonal anti-M. tuberculosis confirma a infecção pelo CMT, mas não é específica, pois pode ocorrer marcação de outras micobactérias. As características histológicas, os achados na coloração de ZN e na IHQ de 13 casos de herbívoros selvagens diagnosticados com tuberculose no Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (SPV-UFRGS) no período de 2003 a 2015 são descritos. A partir das amostras em blocos de parafina foram confeccionadas novas lâminas histológicas, coradas com hematoxilina e eosina (HE) e ZN. Cortes foram submetidos à técnica de IHQ para detecção do CMT. Todos os animais eram adultos, provenientes de cativeiro e incluíram lhama (5/13), cervo sambar (4/13), camelo (1/13), cervo vermelho (1/13), anta brasileira (1/13) e antílope Nilgai (1/13). Na IHQ observou-se imunomarcação acentuada (4/13), moderada (4/13) ou discreta (4/13), exceto em um caso, em que não havia quantidade suficiente de material. As características histológicas, bem como os achados na coloração de ZN e na técnica de IHQ confirmaram o diagnóstico de infecção por Mycobacterium sp. e foram considerados métodos rápidos e eficientes, de forma que podem ajudar na prevenção da disseminação da doença em animais.

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Caracterização histológica e imuno-histoquímica das lesões de tuberculose em bovinos e de linfadenite granulomatosa em suínos

Cited by 9 publications

References 18 publications

Tuberculosis of the central nervous system in cattle in Paraíba, Brazil

Tuberculosis of the central nervous system in cattle in Paraíba, Brazil

Clinical - Pathological Aspects of Hepatocellular Carcinoma in Cattle

Infecção por Mycobacterium sp. em herbívoros selvagens de cativeiro no Rio Grande do Sul: estudo retrospectivo e detecção imuno-histoquímica (2003-2015)

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