Caracterização molecular da resistência aos carbapenêmicos em enterobactérias isoladas em hospitais brasileiros

Aguilar, Mónica Alejandra Pavez

doi:10.11606/d.9.2009.tde-28092009-144325

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…Sample preparation: In order to perform the phenotypic tests, the strains stored in Trypticase Soy Broth plus 15% of glycerol were subcultured in petri dishes containing Mueller Hinton agar, and incubated at 35 ± 2 ºC for 24 h. For the genotypic test, the process of extraction of deoxyribonucleic acid (DNA) was performed by a thermal analysis procedure 38 - 41 . Besides, it should be noted that the subsequent tests were performed in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Investigation of Biofilm Formation in Coagulase-Negative Staphylococci Isolated From Platelet Concentrate Bags

Martini

Hörner

Rampelotto

et al. 2016

Rev. Inst. Med. trop. S. Paulo

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Platelet Concentrates (PCs) are the blood components with the highest rate of bacterial contamination, and coagulase-negative staphylococci (CoNS) are the most frequently isolated contaminants. This study investigated the biofilm formation of 16 contaminated units out of 691 PCs tested by phenotypic and genotypic methods. Adhesion in Borosilicate Tube (ABT) and Congo Red Agar (CRA) tests were used to assess the presence of biofilm. The presence of icaADC genes was assessed by means of the Polymerase Chain Reaction (PCR) technique. With Vitek(r)2, Staphylococcus haemolyticus was considered the most prevalent CoNS (31.25%). The CRA characterized 43.8% as probable biofilm producers, and for the ABT test, 37.5%. The icaADC genes were identified in seven samples by the PCR. The ABT technique showed 85.7% sensitivity and 100% specificity when compared to the reference method (PCR), and presented strong agreement (k = 0.8). This study shows that species identified as PCs contaminants are considered inhabitants of the normal skin flora and they might become important pathogens. The results also lead to the recommendation of ABT use in laboratory routine for detecting biofilm in CoNS contaminants of PCs.

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Section: Methodsmentioning

confidence: 99%

Investigation of Biofilm Formation in Coagulase-Negative Staphylococci Isolated From Platelet Concentrate Bags

Martini

Hörner

Rampelotto

et al. 2016

Rev. Inst. Med. trop. S. Paulo

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“…The microtube was subjected to heating shock (on ice) for 5 minutes and centrifuged at 11,000 rotations per minute (rpm) for 20 minutes. The supernatant (containing the DNA) was transferred to another microtube which was frozen at -20 °C (Nogueira et al, 2004;Freschi, Carvalho, Oliveira, 2005;Scheffer, 2008;Aguilar, 2009). DNA extraction by heating shock was confirmed by the 16S rRNA gene amplification, obtaining a high quality DNA.…”

Section: Analysis Of Resistance Genesmentioning

confidence: 99%

Antimicrobial susceptibility profile and research of mec A and erm genes in coagulase-negative staphylococci isolated from platelet concentrates bags

Martini

Hörner

Graichen³

2017

Braz. J. Pharm. Sci.

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In recent years, several studies have described the clinical impact of bacterial infection associated with transfusion of platelet concentrates (PCs). Among the blood components, PCs are responsible for the highest rates of bacterial contamination as well as septic transfusion reactions. We assessed antimicrobial susceptibility profile, resistance to methicillin (MRCoNS), and resistance to macrolides, lincosamides and streptogramins of group B (MLS B ) of 16 coagulase-negative staphylococci (CoNS) isolates from an investigation in 691 PCs bags. We then compared conventional and automated phenotypic methods, disc diffusion test (DD) and VITEK® 2, respectively as well as phenotypic and genotypic methods (Polymerase Chain Reaction -PCR). All CoNS were susceptible to vancomycin. The disc diffusion test characterized 18.75% as MRCoNS and 37.5% with inducible resistance to MLS B (iMLS B ), and with VITEK® 2, 6.3% and 31.25%, respectively. The mecA gene was detected in 18.75% and the erm gene in 31.25% of the isolates. In this study, we found equal percentage values between presence of the mecA gene by PCR and resistance to methicillin using cefoxitin by DD test, evidence of the erm gene by PCR, and iMLS B resistance by automation (VITEK® 2). Moreover, we identified three strains with beta-lactamase overproduction, and the occurrence of a bigger mistake was verified when automation was compared with DD test. And we observed that D-test was the most reliable for the detection of iMLS B resistance in Staphylococcus sp.

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Caracterização molecular da resistência aos carbapenêmicos em enterobactérias isoladas em hospitais brasileiros

Cited by 2 publications

References 31 publications

Investigation of Biofilm Formation in Coagulase-Negative Staphylococci Isolated From Platelet Concentrate Bags

Investigation of Biofilm Formation in Coagulase-Negative Staphylococci Isolated From Platelet Concentrate Bags

Antimicrobial susceptibility profile and research of mec A and erm genes in coagulase-negative staphylococci isolated from platelet concentrates bags

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