Caracterización de patrones de hordeínas en variedades mexicanas de cebada maltera

Salgado-Albarrán, Marisol; Herrera‐Díaz, Jorge; Dinkova, Tzvetanka D.

doi:10.1016/j.recqb.2015.05.004

Cited by 3 publications

(3 citation statements)

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“…The germinative energy (GE) and the GP of seeds were evaluated according to ISTA rules for seed testing . The different fractions of hordeins were extracted from symptomatic/healthy grains by the method described by Salgado‐Albarrán et al . All proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) ( T = 13.5%).…”

Section: Methodsmentioning

confidence: 99%

“…The C‐hordeins have a size of 55 to 70 kDa, do not contain cysteine residues, so they do not tend to form complexes with other proteins and their abundance is 10–20%. The B‐hordeins have a size of 36 to 44 kDa, are rich in cysteine residues, therefore they can form intrachain or interchain disulfide bridges and are the most abundant (75–80%) . The B‐hordeins represent the primary factor affecting grain protein content, with a negative correlation between the B‐hordein content and malt extract …”

Section: Introductionmentioning

confidence: 99%

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Effects ofFusarium graminearumandFusarium poaeon disease parameters, grain quality and mycotoxin contamination in barley (part II)

et al. 2020

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BACKGROUND Barley is one of the most sown crops in the world, with multiple uses such as human consumption, animal feed and for the malting industry. This crop is affected by different diseases, such as Fusarium Head Blight (FHB), that causes losses in yield and quality. In the last years F. graminearum and F. poae were two of the most frequently isolated species in barley grains, so the aim of this study was to evaluate the interaction between these Fusarium species and the effects on disease parameters, grain quality and mycotoxin contamination on five barley genotypes under field conditions. RESULTS Statistical differences between Fusarium treatments for some parameters depending mainly on the year/genotype were found. The results showed that the germination process was affected by both Fusarium species. As to grain quality and the different hordein fractions, it was observed that F. graminearum affects preferentially D and C‐hordeins. Different concentrations of nivalenol, deoxynivalenol and their acetylated derivatives (3‐acetyl deoxynivalenol (3‐ADON), 15‐acetyl deoxynivalenol (15‐ADON)) were detected. CONCLUSIONS In the present work, no evidence of synergism between F. graminearum and F. poae were found regarding disease parameters and mycotoxin contamination. However, at least in the years with favorable climatic conditions to FHB development and depending on the barley genotype, a continuous monitoring is deemed necessary to prevent the negative impact on protein composition and germinative parameters © 2020 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects ofFusarium graminearumandFusarium poaeon disease parameters, grain quality and mycotoxin contamination in barley (part II)

et al. 2020

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“…Importantly, molecular markers associated with malting quality, protein composition or stress responses are not available for Mexican barley cultivars, which are the major malt source to produce worldwide distributed and recognized Mexican beer brands. An initial study evaluated the hordein protein patterns in the mature seeds of the varieties cultivated in Mexico, revealing some differences between them [ 25 ]. In the same study, more striking differences were found between the malt hordein patterns of cultivars suggesting a distinctive presence or activity of enzymes involved in protein stability/breakdown during germination.…”

Section: Introductionmentioning

confidence: 99%

Protein Disulfide Isomerase (PDI1-1) differential expression and modification in Mexican malting barley cultivars

et al. 2018

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Barley malting quality depends on seed characteristics achieved during grain development and germination. One important parameter is protein accumulation in the mature seed, which may vary between cultivars. Here we conducted a protein pattern analysis in the range of pI 4–7 of mature grains from five Mexican barley cultivars, commonly used for malt and beer production. Reproducibly distinct protein spots, separated by 2D SDS PAGE, were identified by mass spectrometry and considered as potential markers for cultivars with distinct seed protein accumulation. The expression patterns of glutamate decarboxylase (GAD) and protein disulfide isomerase (PDI1-1) were followed at transcript level during grain development for three independent growth cycles to establish whether differences between cultivars were reproducible. Quantitative determination of PDI1-1 protein levels by ELISA confirmed a reproducibly, distinctive accumulation and post-translational modifications between cultivars, which were independent of plant growth regimes. According to its impact on differential storage protein accumulation, we propose the PDI1-1 protein as potential biomarker for Mexican malting barley cultivars.

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Comparison of learning effects between hybrid flipped learning and flipped learning

Choi

2024

JKPTS

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Caracterización de patrones de hordeínas en variedades mexicanas de cebada maltera

Cited by 3 publications

References 20 publications

Effects ofFusarium graminearumandFusarium poaeon disease parameters, grain quality and mycotoxin contamination in barley (part II)

Effects ofFusarium graminearumandFusarium poaeon disease parameters, grain quality and mycotoxin contamination in barley (part II)

Protein Disulfide Isomerase (PDI1-1) differential expression and modification in Mexican malting barley cultivars

Comparison of learning effects between hybrid flipped learning and flipped learning

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