Caracterización microscópica de la dentina de dientes temporales.

Macal, Juan Carlos Martínez; Moreno, Iván Olin

doi:10.35366/102973

Cited by 2 publications

(2 citation statements)

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“…hDPSCs are isolated from a donor’s adult third molars collected during the extraction of wisdom teeth [ 102 ]. Furthermore, considering that dentinal tubules extend from the pulp to the dental enamel [ 134 , 135 , 136 ] and participate in fluid diffusion toward the pulp [ 134 , 137 , 138 ], it was considered necessary to analyze the effects of I. guayusa and P. marginatum extracts on dental pulp. Moreover, in enamel alterations such as AI, in which fluid diffusion presents an additional increase in dentin permeability due to a decrease in its quality or the total loss of enamel, dentin becomes more conductive and exposes the dentin–pulp complex more easily [ 136 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Ilex guayusa and Piper marginatum Extract Cytotoxicity on Human Dental Pulp Mesenchymal Stem Cells

Sequeda-Castañeda,

Suárez-Carvajal,

Téllez-Corral

et al. 2024

Dentistry Journal

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Background: Amelogenesis imperfecta is a hereditary disorder affecting dental enamel. Among its phenotypes, hypocalcified AI is characterized by mineral deficiency, leading to tissue wear and, consequently, dental sensitivity. Excessive fluoride intake (through drinking water, fluoride supplements, toothpaste, or by ingesting products such as pesticides or insecticides) can lead to a condition known as dental fluorosis, which manifests as stains and teeth discoloration affecting their structure. Our recent studies have shown that extracts from Colombian native plants, Ilex guayusa and Piper marginatum, deposit mineral ions such as phosphate and orthophosphate into the dental enamel structure; however, it is unknown whether these extracts produce toxic effects on the dental pulp. Objective: To assess cytotoxicity effects on human dental pulp stem cells (hDPSCs) exposed to extracts isolated from I. guayusa and P. marginatum and, hence, their safety for clinical use. Methods: Raman spectroscopy, fluorescence microscopy, and flow cytometry techniques were employed. For Raman spectroscopy, hDPSCs were seeded onto nanobiochips designed to provide surface-enhanced Raman spectroscopy (SERS effect), which enhances their Raman signal by several orders of magnitude. After eight days in culture, I. guayusa and P. marginatum extracts at different concentrations (10, 50, and 100 ppm) were added. Raman measurements were performed at 0, 12, and 24 h following extract application. Fluorescence microscopy was conducted using an OLIMPUS fv1000 microscope, a live–dead assay was performed using a kit employing a BD FACS Canto TM II flow cytometer, and data analysis was determined using a FlowJo program. Results: The Raman spectroscopy results showed spectra consistent with viable cells. These findings were corroborated using fluorescence microscopy and flow cytometry techniques, confirming high cellular viability. Conclusions: The analyzed extracts exhibited low cytotoxicity, suggesting that they could be safely applied on enamel for remineralization purposes. The use of nanobiochips for SERS effect improved the cell viability assessment.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Ilex guayusa and Piper marginatum Extract Cytotoxicity on Human Dental Pulp Mesenchymal Stem Cells

Sequeda-Castañeda,

Suárez-Carvajal,

Téllez-Corral

et al. 2024

Dentistry Journal

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“…Algunos estudios revelan que, los cristales de los bovinos son más amplios que los de los humanos [13] y, otros, que las estructuras cristalinas del esmalte humano y las especies bovinas son similares [14,15]. La dentina humana como la bovina presenta consistencia dura y es de color ligeramente amarillenta, da forma a todo el diente y contiene una cavidad interna ocupada por la pulpa dentaria [9,16]. La conformación estructural de la dentina de bovino es similar a la reportada para humanos, siendo el principal componente orgánico, observado con microscopía óptica y medio de contraste de Hematoxilina-Eosina, el colágeno tipo I, el mismo de la dentina humana [10].…”

Section: Desarrollo Descripción Microscópicaunclassified

Comparación de las características histológicas entre tejidos dentarios bovino y humano

Falcón,

Enz,

Gili

2023

Rev. nac. odontol.

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Diversos estudios coinciden en que los dientes humanos son similares morfológica e histológicamente a los de algunos mamíferos. En particular, los dientes incisivos bovinos presentan algunas características especiales parecidas a las de los humanos. Existe escasa literatura que describa microscópicamente la estructura dentaria bovina, siendo este un eslabón pendiente para la descripción histológica completa de dicha pieza dentaria, para su potencial uso como sustituta de la humana. El presente manuscrito tiene como objetivo identificar las características histológicas de piezas dentarias humanas y bovinas para su utilización en trabajos de investigación y enseñanza en odontología.

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Caracterización microscópica de la dentina de dientes temporales.

Abstract: Caracterización microscópica de la dentina de dientes temporales.Microscopic characterization of the dentin of temporary teeth.

Cited by 2 publications

References 5 publications

Evaluation of Ilex guayusa and Piper marginatum Extract Cytotoxicity on Human Dental Pulp Mesenchymal Stem Cells

Evaluation of Ilex guayusa and Piper marginatum Extract Cytotoxicity on Human Dental Pulp Mesenchymal Stem Cells

Comparación de las características histológicas entre tejidos dentarios bovino y humano

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