Caracterización molecular de accesiones cultivadas y silvestres de frijol común de Honduras

Abstract

Trabajo conducido como parte de las actividades de los proyectos de investigación auspiciados por el Programa Dry grain Pulses crsP (donación UsaiD no. eDH-a-00-07-00005-00).

Diversidad de frijoles nativos de diferentes regiones del estado de Puebla

“…This case can be applied to results herein obtained mainly in groups formed by one variety (groups VII, IX, X and XII). Guachambala and Rosas (2010), in the dendrogram created with molecular analysis of bean in Honduras found two contrasting scenarios in genetic similarity and collection locations, in one case they create a group with a coefficient of 0.95 in similarity where accessions were collected in very near places and that are very similar, and other case where they create another accessions group that has high genetic similarity with a coefficient of 0.96, but are from distant origin.…”

“…RAPD (Random Amplified Polymorphic DNA) belong to molecular genetic markers used in characterization studies; they are dominant markers and polymorphism among individuals results from changes in sequences in one or both sides of genome acknowledged by one starter which means presence or absence of bands (Rafalski et al, 1994). One of RAPDs uses is to estimate degree of similarity between genotypes, therefore genetic relationships can be defined (Beebe et al, 1995;Skroch et al, 1998;Beebe et al, 2000;Emygdio et al, 2003;Guachambala and Rosas, 2010); this is very useful to know diversity that still exist among genotypes with similar morphological characters, as well as making associations with other attributes of interest for possible assisted selection (Kelly and Miklas, 1998;Jacinto-Hernández et al, 2003) and that would be seized in breeding programs.…”