Caracterización molecular de aislamientos de Escherichia coli productores de toxina Shiga obtenidos en 2 establecimientos ganaderos del Paraguay

Zea, Sofia Marisel Rivelli; Padola, Nora Lía; Etcheverría, Analía Inés; Florentín, Melisa; Acuña, Patricia; Rodríguez, Fátima; Colello, Rocío; Fretes, Rosa María Guillén

doi:10.1016/j.ram.2019.07.001

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…The STEC prevalence reported in studies conducted in Argentina is also within the same range (11.8-38.9%) [38,39,52,53]. However, other authors have reported higher mean STEC prevalence in cattle feces in Paraguay, Canada, Germany, Ireland, UK, France and Australia (44.8-84.8%) [92][93][94][95][96][97][98][99]. International studies have identified differences in cattle stx prevalence according to season, cattle age and feeding practices [48,83,87,97,100,101].…”

Section: Cattle Primary Productionmentioning

confidence: 86%

Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina

et al. 2020

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We developed a quantitative microbiological risk assessment (QMRA) of haemolytic uremic syndrome (HUS) associated with Shiga toxin-producing Escherichia coli (STEC)-contaminated beef (intact beef cuts, ground beef and commercial hamburgers) in children under 15 years of age from Argentina. The QMRA was used to characterize STEC prevalence and concentration levels in each product through the Argentinean beef supply chain, including cattle primary production, cattle transport, processing and storage in the abattoir, retail and home preparation, and consumption. Median HUS probability from beef cut, ground beef and commercial hamburger consumption was <10−15, 5.4x10-8 and 3.5x10-8, respectively. The expected average annual number of HUS cases was 0, 28 and 4, respectively. Risk of infection and HUS probability were sensitive to the type of abattoir, the application or not of Hazard Analysis and Critical Control Points (HACCP) for STEC (HACCP-STEC), stx prevalence in carcasses and trimmings, storage conditions from the abattoir to retailers and home, the joint consumption of salads and beef products, and cooking preference. The QMRA results showed that the probability of HUS was higher if beef cuts (1.7x) and ground beef (1.2x) were from carcasses provided by abattoirs not applying HACCP-STEC. Thus, the use of a single sanitary standard that included the application of HACCP-STEC in all Argentinean abattoirs would greatly reduce HUS incidence. The average number of annual HUS cases estimated by the QMRA (n = 32) would explain about 10.0% of cases in children under 15 years per year in Argentina. Since other routes of contamination can be involved, including those not related to food, further research on the beef production chain, other food chains, person-to-person transmission and outbreak studies should be conducted to reduce the impact of HUS on the child population of Argentina.

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Section: Cattle Primary Productionmentioning

confidence: 86%

Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina

et al. 2020

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“…Considering that there are over 1150 published STEC serotypes, these isolates probably could be assigned to other than the "big six". O103 and O111 serogroups have been previously determined in cattle (Thomas et al, 2012, Bibbal et al, 2015, Jajarmi et al, 2017, Rivelli Zea et al, 2020, their faeces (Blanco et al, 2004, Cernicchiaro et al, 2013 and carcasses (Cap et al, 2019), in countries from South America and other regions. On the other hand, the isolation of STEC O111 in bloody diarrhoea cases has been reported in our country (Varela et al, 2008), which demonstrates a previous circulation of this serogroup.…”

Section: Discussionmentioning

confidence: 99%

Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay

Umpiérrez

Ernst

Cardozo

et al. 2022

Austral j. vet. sci.

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“…For example, STEC O116:H21 was isolated in Argentina from ground beef [ 54 ] and cattle feces [ 55 ]. In Paraguay, O116:H21 was isolated from cattle [ 56 ]. STEC O113:H21 has been isolated in the US and Canada from cattle, pigs, and water obtained in the surroundings of cattle farms [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile

Díaz

Gutiérrez

Switt

et al. 2021

Animals

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Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes tet(A) and tet(R); no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (eae) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the stx2 gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes.

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Caracterización molecular de aislamientos de Escherichia coli productores de toxina Shiga obtenidos en 2 establecimientos ganaderos del Paraguay

Cited by 3 publications

References 8 publications

Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina

Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina

Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay

Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile

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