Caracterización molecular de aislamientos de Shigella sonnei recuperados en el Programa de Vigilancia por el Laboratorio de la Enfermedad Diarreica Aguda en Colombia

Abstract

Realpe: identificación bioquímica y serotipificación María Elena Realpe: coordinación de las actividades del programa de vigilancia por el laboratorio de la enfermedad diarreica aguda del Grupo de Microbiología Todos los autores participaron en el análisis de resultados y la revisión del manuscrito. Caracterización molecular de aislamientos de Molecular characterization of Shigella sonnei isolates recovered by the Laboratory Surveillance Program for Acute Diarrheal Disease in ColombiaIntroduction: In Colombia, Shigella sonnei is one of the most frequently isolated serotypes (53.4%) in human clinical samples associated with diarrheal acute disease. The identification of DNA restriction patterns by pulsed field gel electrophoresis is the basis for the molecular surveillance of S. sonnei. Objective: To establish the basis for the molecular surveillance of S. sonnei in Colombia using pulsedfield gel electrophoresis. Materials and methods:We studied 102 of 2,048 S. sonnei isolates referred by the National Laboratory Network between 1997 and March, 2013; the selection was made according to the antimicrobial multiresistance profile, the source of samples, and the relation to outbreaks. The genetic profile was determined by pulsed field gel electrophoresis using the restriction enzymes XbaI and BlnI in accordance with the PulseNet International protocol. The electrophoretic patterns were analyzed with the GelCompare II, version 4.0 software. Results:We obtained 42 electrophoretic patterns with a 70% to 100% similarity. The most frequent pattern was COIN08J16X01.0017 with 17.6%, followed by patterns COIN04J16X01.0004 with

Eye Contact and Fear of Being Laughed at in a Gaze Discrimination Task

“…In particular, the selection criterion was the participant’s score on the Spanish version of Geloph <15> ( Ruch and Proyer, 2008 ; Carretero-Dios et al, 2010a ). The gelotophobes group consisted of the 20 participants who had the highest trait-gelotophobia scores (18 females; 17–25 years; M Geloph = 2.76; SD Geloph = 0.35; Min Geloph = 2.20; Max Geloph = 3.27).…”

“…The Spanish version of the Geloph <15> ( Ruch and Proyer, 2008 ; Carretero-Dios et al, 2010a ) consists of a self-report questionnaire that assesses trait-gelotophobia, A sample item is “when others laugh in my presence I get suspicious.” It includes 15 positively keyed items in a 4-point answer format ranging from 1 ( Strongly disagree ) to 4 ( Strongly agree ). Test reliability (Cronbach’s alpha) was α = 0.94 in the present sample.…”

“…After performing the experimental task, participants had to fill out, again, the Geloph <15> ( Carretero-Dios et al, 2010a ) to ensure that they had been assigned to the appropriate groups and hence to enhance the validity of the obtained results.…”

Perfiles genéticos bacterianos y análisis de brotes de las Enfermedades Transmitidas por Alimentos empleando Electroforesis de Campo Pulsado como herramienta para la vigilancia epidemiológica molecular

“…Si bien no se pudo confirmar la existencia de un brote esta alerta permitió la intervención de las autoridades responsables. Se logró la construcción de la base de datos nacional de Shigella sonnei, así como lo realizó Velez et al 2015 (13) .…”

“…Infantis; concluyendo que la aplicación de la técnica facilita la investigación epidemiológica de brotes. El estudio de Vélez N et al 2015 (13) determinó la relación genotípica de aislamientos de S. sonnei identificando patrones para la base de datos a nivel nacional mediante PFGE. Ruiz et al 2014 (14) , caracterizó cepas de Campylobacter coli aislados de muestras clínicas, reconociendo varios clusters entre las cepas estudiadas.…”