Caracterización molecular y fenotípica de aislamientos clínicos de Salmonella Typhimurium variante monofásica (1,4,[5],12:i:-) recuperados en Colombia

Cuenca-Arias, Paloma; Montaño, Lucy Angeline; Villarreal, José Miguel; Wiesner, Magdalena

doi:10.7705/biomedica.5417

Cited by 5 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The guinea pig HC100_9460 lineage lacks both the fljA and fljB genes due to the insertion of a phage sequence (STGP_Φ2) but maintains the hin gene. In Colombia and Spain, the most prevalent monophasic variants isolated from humans show the full deletion of the fljAB operon including the hin gene [8,38].…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Salmonella Typhimurium Isolated from Guinea Pigs with Salmonellosis in Lima, Peru

Carhuaricra

Cueva

et al. 2022

Microorganisms

View full text Add to dashboard Cite

Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is one of the most important foodborne pathogens that infect humans globally. The gastrointestinal tracts of animals like pigs, poultry or cattle are the main reservoirs of Salmonella serotypes. Guinea pig meat is an important protein source for Andean countries, but this animal is commonly infected by S. Typhimurium, producing high mortality rates and generating economic losses. Despite its impact on human health, food security, and economy, there is no genomic information about the S. Typhimurium responsible for the guinea pig infections in Peru. Here, we sequence and characterize 11 S. Typhimurium genomes isolated from guinea pigs from four farms in Lima-Peru. We were able to identify two genetic clusters (HC100_9460 and HC100_9757) distinguishable at the H100 level of the Hierarchical Clustering of Core Genome Multi-Locus Sequence Typing (HierCC-cgMLST) scheme with an average of 608 SNPs of distance. All sequences belonged to sequence type 19 (ST19) and HC100_9460 isolates were typed in silico as monophasic variants (1,4,[5],12:i:-) lacking the fljA and fljB genes. Phylogenomic analysis showed that human isolates from Peru were located within the same genetic clusters as guinea pig isolates, suggesting that these lineages can infect both hosts. We identified a genetic antimicrobial resistance cassette carrying the ant(3)-Ia, dfrA15, qacE, and sul1 genes associated with transposons TnAs3 and IS21 within an IncI1 plasmid in one guinea pig isolate, while antimicrobial resistance genes (ARGs) for β-lactam (blaCTX-M-65) and colistin (mcr-1) resistance were detected in Peruvian human-derived isolates. The presence of a virulence plasmid highly similar to the pSLT plasmid (LT2 reference strain) containing the spvRABCD operon was found in all guinea pig isolates. Finally, seven phage sequences (STGP_Φ1 to STGP_Φ7) were identified in guinea pig isolates, distributed according to the genetic lineage (H50 clusters level) and forming part of the specific gene content of each cluster. This study presents, for the first time, the genomic characteristics of S. Typhimurium isolated from guinea pigs in South America, showing particular diversity and genetic elements (plasmids and prophages) that require special attention and also broader studies in different periods of time and locations to determine their impact on human health.

show abstract

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Salmonella Typhimurium Isolated from Guinea Pigs with Salmonellosis in Lima, Peru

Carhuaricra

Cueva

et al. 2022

Microorganisms

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Análise genômica de isolados bacterianos de cobaias (Cavia porcellus) e preguiças-de-garganta-marrom (Bradypus variegatus)

Huaman

View full text Add to dashboard Cite

show abstract

Comparative genomics between Colombian clinical isolates of Monophasic VariantSalmonellaTyphimurium and international clonal lineages

Cuenca-Arias

Valencia

Rodríguez

et al. 2023

Preprint

View full text Add to dashboard Cite

In Colombia,SalmonellaTyphimurium monophasic variant (MVST) is the fourth serovar recovered in laboratory surveillance of acute diarrheal disease (ADD). Given its rapid worldwide dissemination, increasing multidrug-resistance, and the emergence of different endemic clones, it is considered an emerging public health problem. This study compared 21 Colombian clinical isolates and 27 MVST genomes from Europe, Asia, the United States, and Australia to know the gene pool and to define similarities with international clones. Eighty percent of the Colombian MVST isolates formed a lineage divided into 2 clones, while 4 genomes were associated with the European ST34 and USA lineages. These two Colombian clones emerged in relatively recent events, in which possible spread was established during 2011 and 2012, exhibiting a diversity of plasmids and prophages, adapting to the Colombian population after differentiation. These results are a clear example of the high plasticity of MVST, evidencing the need for active genomic surveillance to monitor the circulation of new clonal lineages.

show abstract

Caracterización molecular y fenotípica de aislamientos clínicos de Salmonella Typhimurium variante monofásica (1,4,[5],12:i:-) recuperados en Colombia

Cited by 5 publications

References 40 publications

Genomic Characterization of Salmonella Typhimurium Isolated from Guinea Pigs with Salmonellosis in Lima, Peru

Genomic Characterization of Salmonella Typhimurium Isolated from Guinea Pigs with Salmonellosis in Lima, Peru

Análise genômica de isolados bacterianos de cobaias (Cavia porcellus) e preguiças-de-garganta-marrom (Bradypus variegatus)

Comparative genomics between Colombian clinical isolates of Monophasic VariantSalmonellaTyphimurium and international clonal lineages

Contact Info

Product

Resources

About