Caracterization of glutathione S-transferase of Dermacantor marginatus and effect of the recombinant antigen as a potential anti-tick vaccine

Huercha,; Song, Ruiqi; Li, Min; Fan, Xinli; Hu, Zhengxiang; Wu, Lijiang; Li, Yongchang; Zhang, Wei; Zhang, Yang; Ma, Yanbing; Chahan, Bayin

doi:10.1016/j.vetpar.2020.109043

Cited by 18 publications

(8 citation statements)

References 42 publications

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“…As mentioned in the literature review that a number of tick protective antigens have been found to be useful in tick control (de la Fuente 2018). The tick GSTs were also investigated as potential candidates for vaccination against many tick species, and reached a protective effect by reducing repletion rate and egg production of female adult ticks, and egg hatchability (Huercha et al 2020;Ndawula et al 2019;Oldiges et al 2016;Sabadin et al 2017). The GSTs are a superfamily present in eukaryotes and prokaryotes that are generally involved in the detoxification of endogenous and xenobiotic compounds (Ketterman et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a recombinant GST of Haemaphysalis longicornis Neumann was tested on rabbit against two closely related species Rhipicephalus appendiculatus Neumann and Rhipicephalus sanguineus (Latreille) and cross protection was obtained against adult R. appendiculatus females (Sabadin et al 2017 ). Our previous study using recombinant D. marginatus GST mu-1, a homolog of HlGST, as antigen to protect rabbits showed partial protection toward adult D. marginatus infestation (Huercha et al 2020 ). A more recent study on a delta-class GST of Ixodes ricinus (L.) revealed that the IrGST is induced by heme in blood meal digestion (Perner et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes

Huercha

Hao

et al. 2020

Exp Appl Acarol

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Dermacentor marginatus is a widespread tick species and a vector of many pathogens in Eurasia. Due to the medical importance of D. marginatus, control measures are needed for this tick species. Currently tick control approaches rely mostly on acaricide application, whereas wrong and irrational acaricide use may result in drug resistance and residue problems. Vaccination as an alternative approach for tick control has been proven to be effective towards some tick species. However, immunization against D. marginatus has not yet reached satisfactory protection. The effort of in silico based analysis could predict antigenicity and identify candidates for anti-tick vaccine development. We carried out an in silico analysis of D. marginatus glutathione S-transferases (DmGSTs) in order to identify blood-feeding induced GSTs as antigens that can be used in anti-tick vaccine development. Phylogenetic analysis, linear B-cell epitope prediction, homology modeling, and conformational B-cell epitope mapping on the GST models were performed to identify highly antigenic DmGSTs. Relative gene expressions of the seven GSTs were profiled through real-time quantitative PCR (RT-qPCR) to outline GSTs up-regulated during blood feeding. The phylogenetic analysis indicated that the seven GSTs belonged to four classes of GST, including one in epsilon-class, one in zeta-class, one in omega-class, and four in mu-class. Linear B-cell epitope prediction revealed mu-class GSTs share similar conserved antigenic regions. The conformational B-cell epitope mapped on the homology model of the GSTs displayed that GSTs of mu-class showed stronger antigenicity than that of other classes. RT-qPCR revealed DmGSTM1 and DmGSTM2 were positively related to blood feeding. In sum, the data suggest that DmGSTM1 and DmGSTM2 could be tested for potential antitick vaccine trials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes

Huercha

Hao

et al. 2020

Exp Appl Acarol

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“…Modest cross protection was observed when rabbits immunized with Rhipicephalus microplus cystatin 2c slightly reduced the number of engorging females by 11.5%, adult female engorgement weight by 5.8%, and resulted in damaged gut, salivary glands and ovaries of Rhipicephalus appendiculatus [ 216 ]. Glutathione S-transferase cloned from Dermacentor marginatus used as an antigen induced rabbit immune responses that resulted in approximately and overall 44 % reduction in female tick engorgement, egg mass, and hatching [ 217 ].…”

Section: Anti-tick Vaccination Strategiesmentioning

confidence: 99%

Resistance to Ticks and the Path to Anti-Tick and Transmission Blocking Vaccines

Oosterwijk

Wikel

2021

Vaccines

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The medical and veterinary public health importance of ticks and tick-borne pathogens is increasing due to the expansion of the geographic ranges of both ticks and pathogens, increasing tick populations, growing incidence of tick-borne diseases, emerging tick transmitted pathogens, and continued challenges of achieving effective and sustained tick control. The past decades show an increasing interest in the immune-mediated control of tick infestations and pathogen transmission through the use of vaccines. Bovine tick resistance induced by repeated infestations was reported over a century ago. This review addresses the phenomena and immunological underpinning of resistance to tick infestation by livestock and laboratory animals; the scope of tick countermeasures to host immune defenses; and the impact of genomics, functional genomics, and proteomics on dissecting complex tick–host–pathogen interactions. From early studies utilizing tick tissue extracts to salivary gland derived molecules and components of physiologically important pathways in tick gut and other tissues, an increased understanding of these relationships, over time, impacted the evolution of anti-tick vaccine antigen selection. Novel antigens continue to emerge, including increased interest in the tick microbiome. Anti-tick and transmission blocking vaccines targeting pathogen reservoirs have the potential to disrupt enzootic cycles and reduce human, companion, domestic animal, and wildlife exposure to infected ticks.

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“…The effect of GSTs in detoxification of chemical components of acaricides were shown in R. sanguineus, H. longicornis and R. microplus (Duscher et al, 2014;Nandi et al, 2015;Hernandez et al, 2018). GST has been proposed as anti-tick vaccine due to encouraging results in reducing the number, weight and fertility of ticks post feeding on immunized hosts (Oldiges et al, 2016;Sabadin et al, 2017;Ndawula et al, 2019;Huercha et al, 2020).…”

Section: Detoxification and Defensive Response Modulationmentioning

confidence: 99%

Comparative Proteomic Analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) Tropical and Temperate Lineages: Uncovering Differences During Ehrlichia canis Infection

Sanches

Villar

Couto

et al. 2021

Front. Cell. Infect. Microbiol.

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The tick vector Rhipicephalus sanguineus is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the “temperate” and “tropical” lineages of R. sanguineus (s.l.) is the vector competence to Ehrlichia canis, suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the R. sanguineus tropical and temperate lineages and compares their proteomic profile during E. canis infection. Batches of nymphs from the two lineages were allowed to feed on naïve and experimentally E. canis infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of E. canis before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to E. canis infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial R. sanguineus systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control.

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Caracterization of glutathione S-transferase of Dermacantor marginatus and effect of the recombinant antigen as a potential anti-tick vaccine

Cited by 18 publications

References 42 publications

Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes

Sequence identification and expression profile of seven Dermacentor marginatus glutathione S-transferase genes

Resistance to Ticks and the Path to Anti-Tick and Transmission Blocking Vaccines

Comparative Proteomic Analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) Tropical and Temperate Lineages: Uncovering Differences During Ehrlichia canis Infection

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