Caralluma umbellata Peroxidase: Biochemical Characterization and Its Detoxification Potentials in Comparison with Horseradish Peroxidase

Abstract

Caralluma umbellata peroxidase (CUP) is an acidic heme-containing protein having a molecular weight of ~42 kDa and is specific to guaiacol. It is not a glycoprotein. It was purified to 12.5-fold purity with 6.16 % yield. Its activity is dependent on hydrogen peroxide and has an optimum pH and temperature of 6.2 and 45 °C respectively. It can decolorize dyes, viz., Aniline Blue, Reactive Black 5, and Reactive Blue 19 but not Congo Red, while HRP can decolorize Congo Red also. It has lignin-degrading potentiality as it can decompose veratryl alcohol. Detoxification of phenol was more by CUP compared to HRP while with p-nitrophenol HRP has a greater detoxification rate. Based on our results, CUP was identified to be capable of oxidizing a variety of hazardous substances and also a lignin-degrading plant biocatalyst.

Characterization of Calotropis procera root peroxidase and its potential to mediate remediation of phenolic pollutant from petroleum refinery effluent

“…Residual peroxidase activities (%) were calculated as = (peroxidase activity the presence of metal ion and EDTA and acetone /the peroxidase activity in the absence of metal ion and EDTA and acetone)*100 42 kDa for Caralluma umbellata [28], 43 kDa for M. oleifera leaves [26], and 46.5 kDa for vanilla bean [29].…”

Purification and enzymatic properties of a textile dye-decolourizing peroxidase from Moringa oleifera roots

“…These dyes present different chromophores attached to aromatic rings, which confer the color of each dye. A number of these functional groups are located in ortho or para positions, and this difference in positions may accelerate or reduce the decolorization rate, or even inhibit it [9,46].…”

“…Peroxidases are a class of enzyme with a potential for decolorize synthetic dyes and decontaminate hazardous pollutants in contaminated water and industrial effluents [9,10].The horseradish peroxidase (HRP), for example, is a versatile enzyme effective in the removal of a wide spectrum of aromatic compounds (phenols, biphenols, anilines) and in the degradation and precipitation of important industrial azo dyes [10][11][12]. However, the high cost of commercial HRP has led to recent research to discover cheaper sources of plant peroxidases to be used in the treatment of effluents rich in textile dyes [7].…”