Carbapenemase-Producing Klebsiella Pneumoniae ST258 Activates the T6SS in Response to the Host Metabolite Itaconate to Promote Bacterial Adaptation to the Lung

Wong, Tony T.; Shi, W.B.; Urso, Andreacarola; Annavajhala, Medini K.; Uhlemann, Anne Catrin; Prince, Alice

doi:10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a7735

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“…To explore the possibility that C4-dicarboxylates activate the expression of P. phymatum T6SS-b, we fused the T6SS-b promoter to the reporter gfp gene and investigated its expression. Most recently, a study on the opportunistic pathogen Klebsiella pneumoniae serotype ST258, pointed to the C4-dicarboxylates itaconate, succinate and fumarate as being inducers of T6SS expression ( Wong et al, 2020 ). While P. phymatum T6SS-b was constitutively expressed when cells were grown with the C4-dicarboxylates malate and aspartate as carbon sources, the highest expression of T6SS-b was observed in the presence of the C6 compound citrate.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Paraburkholderia phymatum Type VI Secretion Systems (T6SS) Suggests a Role of T6SS-b in Early Symbiotic Interaction

et al. 2021

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Paraburkholderia phymatum STM815, a rhizobial strain of the Burkholderiaceae family, is able to nodulate a broad range of legumes including the agriculturally important Phaseolus vulgaris (common bean). P. phymatum harbors two type VI Secretion Systems (T6SS-b and T6SS-3) in its genome that contribute to its high interbacterial competitiveness in vitro and in infecting the roots of several legumes. In this study, we show that P. phymatum T6SS-b is found in the genomes of several soil-dwelling plant symbionts and that its expression is induced by the presence of citrate and is higher at 20/28°C compared to 37°C. Conversely, T6SS-3 shows homologies to T6SS clusters found in several pathogenic Burkholderia strains, is more prominently expressed with succinate during stationary phase and at 37°C. In addition, T6SS-b expression was activated in the presence of germinated seeds as well as in P. vulgaris and Mimosa pudica root nodules. Phenotypic analysis of selected deletion mutant strains suggested a role of T6SS-b in motility but not at later stages of the interaction with legumes. In contrast, the T6SS-3 mutant was not affected in any of the free-living and symbiotic phenotypes examined. Thus, P. phymatum T6SS-b is potentially important for the early infection step in the symbiosis with legumes.

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Section: Discussionmentioning

confidence: 99%