Carbapenemase-producing Klebsiella pneumoniae strains in Switzerland: human and non-human settings may share high-risk clones

Campos-Madueno, Edgar I.; Moser, Aline; Jost, Géraldine; Maffioli, Carola; Bodmer, Thomas; Perreten, Vincent; Endimiani, Andrea

doi:10.1016/j.jgar.2022.01.016

Cited by 24 publications

(15 citation statements)

References 49 publications

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“…However, our findings indicated that in the absence of selection for plasmid (antibiotic-free medium), no fitness burden was imposed on IncX3- bla OXA-181 - and IncL- bla OXA - 48- containing host cells. This result is consistent with previous findings ( 7 , 35 , 36 ), but contrasts with others where some IncL plasmid-carrying hosts suffered a fitness cost ( 7 , 37 ). Such discrepancies have been suggested to be associated with the genetic background of host cells; thus, depending on the host cell, a plasmid can produce different fitness effects, from no fitness cost to severe fitness cost ( 38 ).…”

Section: Discussionsupporting

confidence: 93%

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla _OXA-181 and bla _OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

et al. 2022

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Section: Discussionsupporting

confidence: 93%

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla _OXA-181 and bla _OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

et al. 2022

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“…pneumoniae isolated from the USA, Switzerland and Myanmar (99% nucleotide identity, GenBank accession numbers CP021941, CP082992 and AP018834) [15,16]. These plasmids shared a common plasmid backbone with our isolates and possessed a multidrug resistance region harboring bla NDM−1 .…”

Section: Discussionmentioning

confidence: 86%

Dissemination of the high-risk clone ST147 carbapenem-resistant Klebsiella pneumoniae from a local tertiary care hospital in the Republic of Korea

Park

Shin

Kim

et al. 2023

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Background : The emergence of carbapenem-resistant Enterobacterales (CRE) infections is rapidly increasing and represents a serious public threat. In 2020, a total of 16,883 carbapenemase-producing Enterobacterales strains were collected; among these isolates, 21 strains were repeatedly isolated in a local tertiary care hospital. Methods : Antimicrobial susceptibility testing was performed using the broth microdilution method. All 21 strains of CRKP were analyzed by PFGE after XbaI digestion. The 21 CRKP strains were sequenced on the Illumina Miseq and Oxford Nanopore GridION platforms. Results : These 21 CRKP isolates showed an identical antimicrobial resistance profile, such as resistance to ampicillin, carbapenems, cephems, chloramphenicol, fluoroquinolone, macrolides and trimethoprim/sulfamethoxazole. Based on whole-genome analysis, these 21 CRKP isolates shared a common genetic structure (ISAba125-IS630-blaNDM-1-bleMBL) and harbored additional resistance determinants (blaOXA-1, blaCTX-M-15, blaSHV-11, blaSHV-67, aac(6')-Ib-cr, qnrS1, OqxA, OqxB, catB3, mph(A), sul1, and dfrA12) and mutations in the quinolone resistance-determining regions of gyrA (S83I) and parC (S80I). These isolates belonged to the ST147 and KL64 capsular types, which were carried on IncFIB replicon plasmids. The 21 CRKP strains collected from one hospital were divided into five PFGE patterns, and they were closely related with a minimum similarity value of 95.2%. These isolates were found to be highly related based on the presence of between 2 and 27 SNPs. Conclusions : These findings indicate that NDM-1-producing K. pneumoniae ST147 may have been introduced via a common source, implying nosocomial transmission; furthermore, continuous monitoring is necessary to prevent endemic transmission.

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“…In research studies, it has been shown that the spread of the carbapenemase gene occurs across a small number of high-risk clones [ 45 ]. In our study, K. pneumoniae strains belonging to ST39 and K-23, which were rarely isolated from patients and poorly presented in the GenBank database, were able to accumulate plasmids carrying three carbapenemase genes, bla NDM-1 , bla KPC-2 , and bla OXA-48 , simultaneously.…”

Section: Discussionmentioning

confidence: 99%

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes blaNDM-1, blaKPC-2, and blaOXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39

et al. 2023

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Background: Klebsiella pneumoniae, a member of the ESKAPE group of bacterial pathogens, has developed multi-antimicrobial resistance (AMR), including resistance to carbapenems, which has increased alarmingly due to the acquisition of carbapenemase genes located on specific plasmids. Methods: Four clinical K. pneumoniae isolates were collected from four patients of a neuro-intensive care unit in Moscow, Russia, during the point prevalence survey. The AMR phenotype was estimated using the Vitec-2 instrument, and whole genome sequencing (WGS) was done using Illumina and Nanopore technologies. Results: All strains were resistant to beta-lactams, nitrofurans, fluoroquinolones, sulfonamides, aminoglycosides, and tetracyclines. WGS analysis revealed that all strains were closely related to K. pneumoniae ST39, capsular type K-23, with 99.99% chromosome identity. The novelty of the study is the description of the strains carrying simultaneously three large plasmids of the IncHI1B, IncC, and IncFIB groups carrying the carbapenemase genes of three types, blaOXA-48, blaNDM-1, and blaKPC-2, respectively. The first of them, highly identical in all strains, was a hybrid plasmid that combined two regions of the resistance genes (blaOXA-48 and blaTEM-1 + blaCTX-M-15 + blaOXA-1 + catB + qnrS1 + int1) and a region of the virulence genes (iucABCD, iutA, terC, and rmpA2::IS110). Conclusion: The spread of K. pneumoniae strains carrying multiple plasmids conferring resistance even to last-resort antibiotics is of great clinical concern.

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Carbapenemase-producing Klebsiella pneumoniae strains in Switzerland: human and non-human settings may share high-risk clones

Cited by 24 publications

References 49 publications

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla _OXA-181 and bla _OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla _OXA-181 and bla _OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

Dissemination of the high-risk clone ST147 carbapenem-resistant Klebsiella pneumoniae from a local tertiary care hospital in the Republic of Korea

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes blaNDM-1, blaKPC-2, and blaOXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39

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Carbapenemase-producing Klebsiella pneumoniae strains in Switzerland: human and non-human settings may share high-risk clones

Cited by 24 publications

References 49 publications

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla OXA-181 and bla OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla OXA-181 and bla OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

Dissemination of the high-risk clone ST147 carbapenem-resistant Klebsiella pneumoniae from a local tertiary care hospital in the Republic of Korea

High-Molecular-Weight Plasmids Carrying Carbapenemase Genes blaNDM-1, blaKPC-2, and blaOXA-48 Coexisting in Clinical Klebsiella pneumoniae Strains of ST39

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Klebsiella Species and Enterobacter cloacae Isolates Harboring bla _OXA-181 and bla _OXA-48 : Resistome, Fitness Cost, and Plasmid Stability

Klebsiella Species and Enterobacter cloacae Isolates Harboring bla _OXA-181 and bla _OXA-48 : Resistome, Fitness Cost, and Plasmid Stability