In this study, the effect of the cold plastic deformation of a Bridgman anvil at room temperature on the hardness and wear resistance of X160CrMoV12 steel was investigated by utilizing the hardness test, X-ray diffraction (XRD), abrasive emery wear (AEMW) test, optical examination, and scanning electron microscopy (SEM). Three batches of samples were prepared for the experiment: I—as-hardened, II—after hardening with subsequent tempering at 600 °C for 1.5 h, and III— after hardening with subsequent plastic deformation. The hardening of the samples was performed at three temperatures: 1100 °C, 1150 °C, and 1200 °C. The highest content of retained austenite, as much as 69.02%, was observed during hardening at 1200 °C, while 17.36% and 38.14% were formed at lower temperatures, respectively. After tempering (Batch II), the content of residual austenite decreased proportionally by a factor of about seven for each hardening temperature. The effect of plastic deformation (Batch III) is observed, analyzing the hardness of the samples from the surface to the depth, reaching an average hardened depth of 0.08 mm. To evaluate the wear resistance, the surfaces of the three test batches were subjected to an abrasive emery wear test under a 5 N load. Hardened and plastically deformed samples showed higher wear resistance than hardened and tempered samples. The results confirmed that the optimal hardening temperature to achieve the maximum wear resistance of this steel is 1100 °C.