Carbodiimide cross-linked amniotic membranes for cultivation of limbal epithelial cells

Hui‐Kang, David; Lai, Jui‐Yang; Cheng, Hsiao-Yun; Tsai, Chen-Chi; Yeh, Lung‐Kun

doi:10.1016/j.biomaterials.2010.05.034

Cited by 140 publications

(136 citation statements)

References 52 publications

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“…Our previous research has shown that the corneal surface can be reconstructed by ex vivo expanded autologous rabbit LECs cultured on carbodiimide cross-linked AM. 13 The ability of chemically modified biological tissues to preserve keratinocyte progenitor cells is confirmed by immunoconfocal microscopy. More recently, LEC cultures on EDC/NHS-treated AM with a greater extent of cross-linking were found to exhibit enhanced stemness.…”

Section: Results and Discussion Preparation Of Chemically Cross-linkementioning

confidence: 94%

“…33 In our laboratory, collagen nanofiber-based AM materials have been chemically cross-linked by exogenous carbodiimide. 8,13,14 However, a technique involving use of EDC/NHS to catalyze the formation of zero-length covalent cross-links between protein molecules allows only limited cross-linking. Herein, we investigated further use of lysine as a cross-linking bridge to enhance the EDC/NHS cross-linking efficiency of AM collagen while maintaining its biocompatibility.…”

Section: Results and Discussion Preparation Of Chemically Cross-linkementioning

confidence: 99%

“…13 Programmed heating was carried out at 5°C per minute in the temperature range of room temperature to 110°C with an empty aluminum pan as the reference probe. Resistance against thermal denaturation (ie, shrinkage temperature) was measured as the onset value of the occurring endothermic peak.…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

“…12 In our laboratory, the EDC/N-hydroxysuccinimide (NHS) has been previously used for the development of chemically cross-linked AM materials. 13 However, with carbodiimide treatment for a longer duration (ie, 4 hours), the AM samples showed significant weight loss after 4 weeks of incubation in MMPs, suggesting low cross-linking efficiency of biological tissues. 14 It is highly desirable to generate cross-links sufficient to provide protective barriers shielding the proteinaceous matrix from enzymatic attack while maintaining its biocompatibility.…”

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confidence: 99%

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Stabilization of collagen nanofibers with l-lysine improves the ability of carbodiimide cross-linked amniotic membranes to preserve limbal epithelial progenitor cells

Lai¹,

Wang²,

Luo³

et al. 2014

IJN

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To overcome the drawbacks associated with limited cross-linking efficiency of carbodiimide modified amniotic membrane, this study investigated the use of l -lysine as an additional amino acid bridge to enhance the stability of a nanofibrous tissue matrix for a limbal epithelial cell culture platform. Results of ninhydrin assays and zeta potential measurements showed that the amount of positively charged amino acid residues incorporated into the tissue collagen chains is highly correlated with the l -lysine-pretreated concentration. The cross-linked structure and hydrophilicity of amniotic membrane scaffolding materials affected by the lysine molecular bridging effects were determined. With an increase in the l -lysine-pretreated concentration from 1 to 30 mM, the cross-linking density was significantly increased and water content was markedly decreased. The variations in resistance to thermal denaturation and enzymatic degradation were in accordance with the number of cross-links per unit mass of amniotic membrane, indicating l -lysine-modulated stabilization of collagen molecules. It was also noteworthy that the carbodiimide cross-linked tissue samples prepared using a relatively high l -lysine-pretreated concentration (ie, 30 mM) appeared to have decreased light transmittance and biocompatibility, probably due to the influence of a large nanofiber size and a high charge density. The rise in stemness gene and protein expression levels was dependent on improved cross-link formation, suggesting the crucial role of amino acid bridges in constructing suitable scaffolds to preserve limbal progenitor cells. It is concluded that mild to moderate pretreatment conditions (ie, 3–10 mM l -lysine) can provide a useful strategy to assist in the development of carbodiimide cross-linked amniotic membrane as a stable stem cell niche for corneal epithelial tissue engineering.

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Section: Results and Discussion Preparation Of Chemically Cross-linkementioning

confidence: 94%

Section: Results and Discussion Preparation Of Chemically Cross-linkementioning

confidence: 99%

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

mentioning

confidence: 99%

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Stabilization of collagen nanofibers with l-lysine improves the ability of carbodiimide cross-linked amniotic membranes to preserve limbal epithelial progenitor cells

Lai¹,

Wang²,

Luo³

et al. 2014

IJN

Self Cite

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“…The possibility of cross-linking it using carbodiimide chemistry is reported to increase the mechanical properties while maintaining its cell-binding capabilities and reducing its degradation. [22] An alternative strategy has involved developing a composite scaffold made of a double layer of amniotic membrane glued together with fibrin which had increased mechanical properties and performed well in a rabbit ocular reconstruction model. [23] An alternative natural tissue that has been investigated is decellularized pig corneas.…”

Section: Amniotic Membranementioning

confidence: 99%

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

Williams

Lace

Kennedy

et al. 2018

Adv Healthcare Materials

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The role of biomaterials in tissue engineering and regenerative medicine strategies to treat vision loss associated with damage to tissues in the anterior segment of the eye has been studied for several years. This has mostly involved replacement and support for the cornea and conjunctiva. These are complex tissues with specific functional requirements for different parts of the tissue. Amniotic membrane (AM) is used in clinical practice to transplant autologous or allogenic cells to the corneal surface. Fibrin gels have also progressed to clinical use under specific conditions. Alternatives to AM such as collagen gels, other natural materials, for example keratin and silks, and synthetic polymers have received considerable attention in laboratory and animal studies. This experience is building a body of evidence to demonstrate the potential of tissue engineering and regenerative medicine in corneal and conjunctival reconstruction and can also lead to other applications in the anterior segment of the eye, for example, the trabecular meshwork. There is a real clinical need for new procedures to overcome vision loss but there are also opportunities for developments in ocular applications to lead to biomaterials innovations for use in other clinical areas.

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Development of a Cost‐Effective and Simple Protocol for Decellularization and Preservation of Human Amniotic Membrane as a Soft Tissue Replacement and Delivery System for Bone Marrow Stromal Cells

Gholipourmalekabadi

Mozafari

Salehi

et al. 2015

Adv Healthcare Materials

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The aim of this study is to develop a simple and cost-effective method for decellularization and preservation of human amniotic membrane (HAM) as a soft tissue replacement and a delivery system for stem cells. The HAM is decellularized (D) using new chemical and mechanical techniques. The decellularization scaffold is evaluated histologically and fully characterized. The cell adhesion and proliferation on the scaffold are also investigated and the biocompatibility of D tissues is evaluated in vivo. The histological studies reveal that the cells are successfully removed from the D tissue. The DNA extraction shows more than 95% cell removal (p = 0.001). The in vitro results indicate that the decellularisation process does not deteriorate the mechanical properties of the tissue, whereas it increases the in vitro biodegradation value (p < 0.05). In the D samples, there is no significant cytotoxicity, and no changes are found in the rate of cell proliferation (p > 0.05). Immunohistochemistry staining indicates that all the tested components remain unchanged within the D tissues. The count of inflammatory cells show that the decellularization process slightly increases the biocompatibility of the tissue after 7 days post-surgery. The results indicate that scaffold proves to be reproducible, rapid, and cost-effective, with a potential role for clinical application.

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Carbodiimide cross-linked amniotic membranes for cultivation of limbal epithelial cells

Cited by 140 publications

References 52 publications

Stabilization of collagen nanofibers with l-lysine improves the ability of carbodiimide cross-linked amniotic membranes to preserve limbal epithelial progenitor cells

Stabilization of collagen nanofibers with l-lysine improves the ability of carbodiimide cross-linked amniotic membranes to preserve limbal epithelial progenitor cells

Biomaterials for Regenerative Medicine Approaches for the Anterior Segment of the Eye

Development of a Cost‐Effective and Simple Protocol for Decellularization and Preservation of Human Amniotic Membrane as a Soft Tissue Replacement and Delivery System for Bone Marrow Stromal Cells

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