Carbohydrases: 50 Years of Research at the Department of Chemical Enzymology of Moscow State University—History and Prospects

Sinitsyn, A. P.; Sinitsyna, O. A.; Zorov, I. N.; Rozhkova, A. M.

doi:10.3103/s0027131423040065

Cited by 3 publications

(1 citation statement)

References 103 publications

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“…Currently, recombinant GHs produced by Penicillium fungi have received large-scale development and have demonstrated high efficiency in long-term studies [24,25] towards different cellulosic substrates [26], including good performance on kraft pulps [13,15,27]. In the 1990s, researchers of the Department of Chemical Enzymology of the Moscow State University started the development of laboratory-scale and industrial enzyme mixture derived from highly active P. verruculosum strains, which have already found applications as feed additives in animal breeding, increasing nutritional value [28,29]. The optimization of P. verruculosum enzyme compositions creates prospects for the successful saccharification of wood lignocellulose.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?

Shevchenko,

Mayorova,

Chukhchin

et al. 2023

Fermentation

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Sulfite and kraft pulping are two principal methods of industrial delignification of wood. In recent decades, those have been considered as possibilities to pretreat recalcitrant wood lignocellulosics for the enzymatic hydrolysis of polysaccharides and the subsequent fermentation of obtained sugars to valuable bioproducts. Current work compares chemistry and technological features of two different cooking processes in the preparation of polysaccharide substrates for deep saccharification with P. verruculosum glycosyl hydrolases. Bleached kraft and sulfite pulps were subjected to hydrolysis with enzyme mixture of high xylanase, cellobiohydrolase, and β-glucosidase activities at a dosage of 10 FPU/g of dry pulp and fiber concentration of 2.5, 5, and 10%. HPLC was used to analyze soluble sugars after hydrolysis and additional acid inversion of oligomers to monosaccharides. Kraft pulp demonstrated higher pulp conversion after 48 h (74–99%), which mostly resulted from deep xylan hydrolysis. Sulfite-pulp hydrolysates, obtained in similar conditions due to higher hexose concentration (more than 50 g/L), had higher fermentability for industrial strains producing alcohols, microbial protein, or organic acids. Along with saccharification, enzymatic modification of non-hydrolyzed residues occurred, which led to decreased degree of polymerization and composition changes in two industrial pulps. As a result, crystallinity of kraft pulp increased by 1.3%, which opens possibilities for obtaining new types of cellulosic products in the pulp and paper industry. The high adaptability and controllability of enzymatic and fermentation processes creates prospects for the modernization of existing factories.

show abstract

Section: Introductionmentioning

confidence: 99%

Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?

Shevchenko,

Mayorova,

Chukhchin

et al. 2023

Fermentation

View full text Add to dashboard Cite

show abstract

Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?

Shevchenko,

Mayorova,

Chukhchin

et al. 2023

Preprint

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Sulfite and kraft pulping are two principal methods of industrial delignification of wood. In recent decades, those have been considered as possibilities to pretreat recalcitrant wood lignocellulosics for enzymatic hydrolysis of polysaccharides and subsequent fermentation of obtained sugars to valuable bioproducts. Current work compares chemistry and technological features of two different cooking processes in the preparation of polysaccharide substrates for deep sacchari-fication with P. verruculosum glycosyl hydrolases. Bleached kraft and sulfite pulps were subjected to hydrolysis with enzyme mixture of high xylanase, cellobiohydrolase and β-glucosidase activities at a dosage of 10 FPU/g of dry pulp and fiber concentration of 2.5, 5 and 10%. HPLC was used to analyze soluble sugars after hydrolysis and additional acid inversion of oligomers to monosaccharides. Kraft pulp demonstrated higher pulp conversion after 48 h (74-99%), mostly resulted from deep xylan hydrolysis. Sulfite-pulp hydrolysates, obtained in similar conditions, due to higher hexose concentration (more than 50 g/L), had higher fermentability for industrial strains producing alcohols, microbial protein or organic acids. Along with saccharification, enzymatic modification of non-hydrolysed residues occurred, which led to decreased degree of polymerization and composition changes in two industrial pulps. As a result, crystallinity of kraft pulp increased by 1.3%, which opens possibilities for obtaining new types of cellulosic products in pulp and paper industry. The high adaptability and controllability of enzymatic and fermentation processes creates prospects for the modernization of existing factories.

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Biocatalysis: modern problems and applications

Varfolomeev,

Švedas,

Efremenko

et al. 2024

RUSS CHEM REV

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The review is devoted to analysis of recent advances, problems, and practical applications of enzymes in the framework of chemical enzymology and enzyme engineering. The achievements in the fundamental understanding of the molecular mechanisms of the catalytic cycle of enzymatic reactions are considered using quantum/molecular mechanics methods with supercomputer technologies and bioinformatics methods. The construction of protein biocatalysts with new properties is a fundamentally significant methodology of the bioengineering approach to solving practical problems, which is demonstrated by a number of examples. The increasing role of biocatalysis in medicine and biomedical research is illustrated by solving the problems of antibiotic synthesis and overcoming bacterial resistance to them, studying the mechanisms of neurodegenerative diseases and developing drugs to treat Alzheimer's disease, and investigation of biocatalytic processes of DNA repair and the role of mechanisms of functioning of heme peroxidases in the human body. The use of enzymes that destroy various endogenous and exogenous toxicants has been greatly developed in recent decades. The problems of using enzymes in therapy and drug delivery are analyzed. The fundamental role of enzymes in modern analysis and diagnosis is noted. The review considers a new trend in the development of bioanalytical methods using aptamers, multianalytical biochips, surface-enhanced Raman scattering systems, and bioelectroanalysis.<br> The bibliography includes 460 references.

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Carbohydrases: 50 Years of Research at the Department of Chemical Enzymology of Moscow State University—History and Prospects

Cited by 3 publications

References 103 publications

Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?

Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?

Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?

Biocatalysis: modern problems and applications

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