Carbohydrate-active enzymes in animal feed

Plouhinec, Lauriane; Neugnot, Virginie; Lafond, Mickaël; Berrin, Jean-Guy

doi:10.1016/j.biotechadv.2023.108145

Cited by 15 publications

(3 citation statements)

References 223 publications

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“…However, these feed constituents, known as non-starch polysaccharides (NSP), are indigestible to broilers. Corn contains approximately 0.9% soluble NSP and 6 to 8% insoluble NSP, whereas soybean meal contains approximately 6% soluble NSP and 18 to 21% insoluble NSP [5,6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Addition of Humicola Grisea Cellulase to Broiler Chicken Rations for a 21-Day Period

Souza,

Ulhoa,

Silva

et al. 2023

Poultry

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This study aimed to evaluate the addition of liquid cellulose, produced by Humicola grisea, in 21-day-old broiler chickens’ diets. The treatments comprised control rations of corn and soybean meal and rations to which 500 mL/t and 1000 mL/t of cellulase were added. A total of 180 male broiler chickens were used, distributed in a completely randomized design, with three treatments and six replicates. Broiler chicken performance was monitored during the period from 1 to 21 days old. Significant effects were detected for digestibility only between four and seven days old, when a reduced dry matter nitrogen intake was recorded, and for nitrogen digestibility in the broilers fed cellulase-supplemented rations at a dose of 1000 m/L. Among the analyzed digestive organs, only the biometrics of the large intestine were affected significantly at seven days old. The absolute weights of the liver and pancreas and the activities of amylase, alkaline phosphatase, and transaminases were not affected significantly, indicating that cellulase did not affect the metabolism of these organs. No significant effect was detected in the serum for electrolytes, total protein, or alkaline phosphatase. So, the addition of liquid cellulase produced by Humicola grisea did not affect performance and metabolism in 21-day-old broiler chickens.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the Addition of Humicola Grisea Cellulase to Broiler Chicken Rations for a 21-Day Period

Souza,

Ulhoa,

Silva

et al. 2023

Poultry

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“…Carbohydrate-active enzymes (CAZymes) are enzymes that primarily target glycosidic bonds and are involved in degrading, synthesizing, or modifying carbohydrates [10]. CAZymes are classified into six categories: glycoside hydrolases (GHs), polysaccharide lyases (PLs), carbohydrate esterases (CEs), glycosyl transferases (GTs), carbohydratebinding modules (CBMs), and auxiliary activities (AAs).…”

Section: Introductionmentioning

confidence: 99%

Metagenomics-Based Analysis of the Effect of Rice Straw Substitution for a Proportion of Whole-Plant Corn Silage on the Rumen Flora Structure and Carbohydrate-Active Enzymes (CAZymes)

Ma,

Ye,

Cheng

et al. 2023

Fermentation

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The purpose of this study was to investigate the effects of replacing a portion of whole-plant corn silage with straw on the rumen microbial community structure and carbohydrate-active enzyme activity. The experiment employed a single-factor randomized trial design, with eight late-lactation Chinese Holstein dairy cows being randomly divided into two groups of four replicates each. The control group (CS group) was fed a diet consisting of alfalfa silage and a mixture of alfalfa and whole-plant corn silage, while the experimental group (RS group) received a diet in which one-third of the corn silage was replaced with straw while keeping the other components unchanged. The experiment lasted for a total of 21 days, with a pre-feeding period of 14 days and a formal period of 7 days. The rumen fluid collected on day 21 was used for the rumen fermentation parameters and metagenomic analysis. The concentrations of acetic acid, propionic acid, butyric acid, and total volatile fatty acids (TVFA) in the rumen of RS group cows were significantly lower than those in the CS group (p < 0.01). The ratio of acetic acid to propionic acid was significantly higher in the RS group compared to the CS group (p < 0.01). Metagenomic sequencing revealed that at the genus level, compared to the CS group, the abundance of unclassified bacteria, Bacteroides, Alistipes, Butyrivibrio, Chlamydia, Fibrobacter, unclassified Ruminococcaceae, and unclassified Bacteroidetes in the rumen of RS group cows increased, while the abundance of Eubacterium decreased ([LDA > 3.6], p < 0.05). Compared to the CS group, the enzymatic activities of α-L-arabinofuranosidase (EC3.2.1.55), β-xylosidase (EC3.2.1.37), β-glucosidase (EC3.2.1.21), β-glucosylceramidase (EC3.2.1.45), xylanase (EC3.2.1.8), and arabinanase (EC3.2.1.99) in the rumen of RS group cows increased (p < 0.05). According to the correlation analysis, Alistipes, Bacteroides, and Butyrivibrio showed a significant negative correlation with propionic acid (p < 0.05) and a significant positive correlation with the acetic acid-to-propionic acid ratio (p < 0.05). They also showed a significant positive correlation with GH2, GH3, GH20, GH29, GH43, GH78, GH92, CE1, GT4, β-glucosidase (EC3.2.1.21), α-L-arabinofuranosidase (EC 3.2.1.55), β-xylosidase (EC 3.2.1.37), β-glucosylceramidase (EC 3.2.1.45), xylanase (EC 3.2.1.8), and arabinanase (EC 3.2.1.99) (p < 0.05). In summary, straw can not only alter the composition and structure of the rumen microbiota in cows but also affect the relative abundance of CAZymes at different levels within the rumen. Cows may, thus, potentially improve the degradation efficiency of straw diets by increasing the abundance of certain rumen microbiota and enzymes.

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“…Enzymes have unique catalytic behavior and are widely studied in different catalysis processes (dos Santos et al, 2014;Kurbanoglu et al, 2020;Virgen-Ortíz et al, 2019). Enzymes can act in different types of reactions of industrial interest, such as esterification, transesterification, C-C bond formation, and alcoholysis (Belle and Nijnik, 2014;Prajapati et al, 2022;Liu et al;González-Davis et al, 2023;Plouhinec et al, 2023;Yamaguchi and Miyazaki, 2023).…”

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confidence: 99%