Carbohydrate Analysis: Can We Control the Ripening of Bananas?

Deal, S. Todd; Farmer, Catherine E.; Cerpovicz, Paul F.

doi:10.1021/ed079p479

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…Each pair of students was assigned either a green, yellow, or overripe banana, and then calculated the percentage of starch and free sugar content in relation to the overall mass. In the first iteration of the experiment, published in 2002, students first separated the soluble and insoluble components of the banana using glass homogenizers and refrigerated centrifuges . A later modification showed that homogenization could be performed as effectively using a mortar and pestle, and tabletop microcentrifuges .…”

Section: General Chemistrymentioning

confidence: 99%

“…In the first iteration of the experiment, published in 2002, students first separated the soluble and insoluble components of the banana using glass homogenizers and refrigerated centrifuges. 34 A later modification showed that homogenization could be performed as effectively using a mortar and pestle, and tabletop microcentrifuges. 35 Pedagogically, this experiment ties together students' familiarity with food science and chemical analysis.…”

Section: Analysis Of Sugar Content In Foodmentioning

confidence: 99%

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Incorporating Carbohydrates into Laboratory Curricula

Koviach-Côté

Pirinelli

2018

Chem. Rev.

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As an important but underappreciated field, carbohydrate chemistry is a critical topic for undergraduate students to learn. With applications to nutrition, food science, and diabetes, carbohydrates provide real-world relevance to students. This review summarizes the literature of undergraduate laboratory experiments which use carbohydrates since the year 2000. Experiments explore important chemical concepts such as synthesis, kinetics, analysis, and computational chemistry. Experiments designed for general chemistry, organic chemistry, biochemistry, and analytical chemistry at a variety of skill levels are presented.

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Section: General Chemistrymentioning

confidence: 99%

Section: Analysis Of Sugar Content In Foodmentioning

confidence: 99%

Incorporating Carbohydrates into Laboratory Curricula

Koviach-Côté

Pirinelli

2018

Chem. Rev.

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“…As a burgeoning field within biochemistry, the significance and roles of carbohydrates beyond metabolism, including current laboratory approaches to this class of biomolecules, is important for undergraduates to effectively pursue the careers growing in this area . At present, instructors have a very limited range of instructional materials available to introduce glycoproteins and carbohydrates into an undergraduate laboratory. − Many of these efforts focus on using well-established but dated techniques, such as the iodine test, thin-layer chromatography, or colorimetric assays, for the indication of carbohydrates. − Only a few educational biochemistry experiments published in the last two decades have incorporated more modern methods and techniques. − …”

Section: Introductionmentioning

confidence: 99%

Protein N-Glycans: Incorporating Glycochemistry into the Undergraduate Laboratory Curriculum

et al. 2018

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Glycoscience continues to be an underrepresented topic in current undergraduate-biochemistry-laboratory curricula. Of the educational laboratories present in this subject area, few introduce students to cutting-edge methods and techniques related to carbohydrates. A multiweek series of experiments is described that highlights a recently published bleach-mediated-carbohydrate-cleavage protocol in the context of the isolation and identification of the soybean glycoprotein, βconglycinin. Two different levels of undergraduate biochemistry courses (a first-year introductory course and an upper-level bioanalytical course) completed the experimental set. Although the same sequence of experiments took place for both levels, the teaching approaches to the material varied. The introductory course used an inquiry-type learning approach, whereas the upper-level students were taught with a guided-inquiry approach. Students in both courses were able to successfully isolate glycosylated β-conglycinin from inexpensive soy flour and then analyze the protein with SDS-PAGE and mass spectrometry. Once β-conglycinin was isolated, students were able to effectively adapt a recently published bleach-mediated-carbohydrate-cleavage protocol on this previously untested glycoprotein and quantitate the carbohydrate content with a colorimetric phenol−sulfuric acid assay. The effective execution of this biochemistry-laboratory series demonstrates an alternative undergraduate-laboratory curriculum that introduces students to traditional glycoscience and protein methods along with more cutting-edge techniques in these areas.

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“…Efforts to replace textbook lab experiment content in traditionally structured organic lab courses were accompanied by motivation to design experiments that would not only bring elements of research into the course but also engage students through applications of target molecules they might find interesting. The field of carbohydrates is rich in synthetic and medical applications , and examples where carbohydrates were integrated into introductory organic chemistry laboratory courses typically involved individual reactions , synthetic sequences , nuclear magnetic resonance (NMR) spectroscopy , or varied applications such as determining sugar content in food or kinetic studies .…”

Section: Introductionmentioning

confidence: 99%

Bringing research into a first semester organic chemistry laboratory with the multistep synthesis of carbohydrate‐based HIV inhibitor mimics

Pontrello

2015

Biochem Molecular Bio Educ

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Benefits of incorporating research experiences into laboratory courses have been well documented, yet examples of research projects designed for the first semester introductory organic chemistry lab course are extremely rare. To address this deficiency, a Carbohydrate-Based human immunodeficiency virus (HIV) Inhibitor project consisting of a synthetic scheme of four reactions was developed for and implemented in the first semester organic lab. Students carried out the synthetic reactions during the last 6 of 10 total labs in the course, generating carbohydrate-based dimeric target molecules modeled after published dimers with application in HIV therapy. The project was designed to provide a research experience through use of literature procedures for reactions performed, exploration of variation in linker length in the target structure, and synthesis of compounds not previously reported in the scientific literature. Project assessment revealed strong student support, indicating enhanced engagement and interest in the course as a direct result of the use of scientific literature and the applications of the synthesized carbohydrate-based molecules. Regardless of discussed challenges in designing a research project for the first semester lab course, the finding from data analysis that a project implemented in the first semester lab had significantly greater student impact than a second semester project should provide motivation for development of additional research projects for a first semester organic course.

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Carbohydrate Analysis: Can We Control the Ripening of Bananas?

Cited by 3 publications

References 3 publications

Incorporating Carbohydrates into Laboratory Curricula

Incorporating Carbohydrates into Laboratory Curricula

Protein N-Glycans: Incorporating Glycochemistry into the Undergraduate Laboratory Curriculum

Bringing research into a first semester organic chemistry laboratory with the multistep synthesis of carbohydrate‐based HIV inhibitor mimics

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