Carbohydrate analysis of hemicelluloses by gas chromatography–mass spectrometry of acetylated methyl glycosides

Abstract

A method based on gas chromatography-mass spectrometry analysis of acetylated methyl glycosides was developed in order to analyze monosaccharides obtained from various hemicelluloses. The derivatives of monosaccharide standards, arabinose, glucose, and xylose were studied in detail and (13)C-labeled analogues were used for identification and quantitative analysis. Excellent chromatographic separation of the monosaccharide derivatives was found and identification of the anomeric configuration was feasible through a prepared and identified pure methyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside. The electron ionization mass spectrum and fragmentation path was studied for each monosaccharide derivative. Fragment ion pairs of labeled and unlabeled monosaccharides were used for quantification; m/z 243/248 for glucose, 128/132 for xylose, and 217/218 for arabinose. Using the intensity ratios obtained from the extracted ion chromatograms, accurate quantification of monosaccharide constituents of selected hemicelluloses was demonstrated.

Kinetic Parameters for Biomass under Self-Ignition Conditions: Low-Temperature Oxidation and Pyrolysis

“…Acid methanolysis and gas chromatography–mass spectrometry (GC–MS) were applied to three of the biomass samples to separate and quantify their hemicellulose (HC)-bound glucose content. The method is described elsewhere in more detail . The analysis was done in duplicate and the results were repeatable within 10% of the reported values.…”

“…The method is described elsewhere in more detail. 38 The analysis was done in duplicate and results were repeatable within 10 % of the reported values. Data is included in Table 1, based on the total dry matter.…”

Characterization of Xylan-graft-Polycaprolactone Copolymers Prepared in Ionic Liquid

“…One of the most abundant hemicelluloses is xylan. 3,5,6 In many plants, xylans are heteropolysaccharides with homopolymeric backbone chains of 1,4-linked β-D-xylopyranose units. 7 Besides xylose, xylan may also contain O-acetyl, α-L-arabinofuranosyl, α-1,2-linked glucuronic, or 4-O-methlglucuronic acid substituents.…”

Ionic Liquid as Green Solvent for Ring-Opening Graft Polymerizaion of ε-Caprolactone onto Hemicelluloses

“…In their natural state, hemicelluloses are generally considered to be noncrystalline, with a DP of 80 to 200.They are heterogeneous polymers of pentose (xylose, arabinose), hexoses (mannose, glucose, and galactose), and sugar acids. Xylans are the most abundant hemicelluloses [8]. In many plant materials, xylans are heteropolysaccharides with homopolymeric backbone chains of 1,4-linked β-D-xylopyranose (Xylp) units [9].…”