Carbohydrate Binding Macromolecules in Intraspecific Marine Microbial Associations

Abstract

High molecular weight exudates that enhance the adhesion of t h e marine ChZoreZZa vulgaris Beij. t o g l a s s h a v e b e e n i s o l a t e d d i r e c t l y f r o m t h e c u l t u r e media of t h i s micro alga and marine bacterial s t r a i n s by iuununoaffinity chromatography. These materials s i g n i f i c a n t l y i n c r e a s e t h e a d h e s i o n of ChZorezkz a t c o n c e n t r a t i o n s o f g / c e l l . The a d h e s i o n a c t i v i t y of e x u d a t e a n t i g e n i s o l a t e d f r o m ChbreZ7.u media and t h e media of a b a c t e r i a l s t r a i n c l o s e l y a s s o c i a t e d w i t h t h i s a l g a l c e l l i n n a t u r e is n o t i n h i b i t e d in the presence of D(+) mannose while exudate antigens recovered from the media of b a c t e r i a l s t r a i n s n o t a s s o c i a t e d w i t h t h i s m i c r o algae are i n h i b i t e d by mannose. The r e l a t i o n s h i p b e t w e e n t h e s e d i f f e r e n c e s i n s u g a r s p e c i f i c i t y a n d micro algal -b a c t e r i a l s u r f a c e i n t e r a c t i o n s i s discussed.

Adhesion of Lactobacillus amylovorus to Insoluble and Derivatized Cornstarch Granules

“…The degree to which starch is accessible in the hydrophobic plastic matrix largely influences the ability of amylolytic bacteria to attach to the plastic surface, as well as availability of starch to hydrolytic enzymes (10,13). Adhesive interactions of microorganisms with insoluble polymeric surfaces is an important phenomenon implicated in a variety of biochemical responses at both a cellular and a molecular level (2,3,7,11,12,15,23,26). It has been suggested that the binding of cellulolytic bacteria to plant cell wall and cellulose matrix facilitate cellulose fiber degradation (4)(5)(6)22).…”

Adhesive Properties of a Symbiotic Bacterium from a Wood-Boring Marine Shipworm

“…These materials have been recovered from coastal seawater and from cultures of marine algae and bacteria (30,31). In the marine environment, symbiotic associations between microorganisms can be regulated by specific cell surface molecules (12,13).…”

Specificity of marine microbial surface interactions

“…The marine alga Chlorella vulgaris (Beij.) is specifically associated with a gram-positive coccoid bacterium in situ (19;Tosteson et al,in press). Both of these microorganisms produce HMW materials that have a lectin-like activity.…”

“…MATERIALS AND METHODS HMW exudates produced by microorganisms have been recovered from their laboratory culture media by dialysis (34). In general, the concentrations of HMW exudate materials recovered from these laboratory cultures ranged from nanograms to micrograms per milliliter of cell-free medium (19,34). To facilitate the isolation of the microbial exudates examined in this study and reduce the time required, we used immunological techniques.…”

“…The NAS-OTEC strain was isolated from the aluminum heat exchanger surfaces at the Puerto Rico OTEC experimental facility (36). On the basis of morphological and biochemical characteristics, the gram-positive associated bacterial strain ASB1 was identified as belonging to the genus Micrococcus (19). The nonassociated bacterial strains NAS-E. coli and NAS-OTEC were both gram negative.…”