Carbohydrate Binding Specificity of the Neutrophil-activating Protein of Helicobacter pylori

Abstract

The possible interaction of the neutrophil-activating protein of Helicobacter pylori with target cell glycoconjugates was investigated by the binding of 125 I-labeled recombinant protein to glycosphingolipids from human neutrophils in solid phase assays. Thereby, a distinct binding of the neutrophil-activating protein to four bands in the acid glycosphingolipid fraction from human neutrophils was detected, whereas no binding to the non-acid glycosphingolipids or polyglycosyl ceramides from these cells was obtained. When using glycosphingolipids not present in the cell membrane of human neutrophils, it was found that the neutrophilactivating protein also bound to sulfated glycosphingolipids as sulfatide and sulfated gangliotetraosyl ceramide. Comparison of the binding preferences of the protein to reference glycosphingolipids from other sources suggested that in human granulocytes, the neutrophil-activating protein of H. pylori preferentially recognizes glycoconjugates with a terminally unsubstituted NeuAc␣3Gal␤4GlcNAc␤3Gal␤4GlcNAc␤ sequence.

Regulation of Vacuolar pH and Its Modulation by Some Microbial Species

“…Despite the lag imposed by the effector delivered by the type IV secretion system, bacteria bound to phagocytes via one or more adhesins (25)(26)(27)120) are nevertheless internalized and found in phagosomes (46,71,139). Remarkably, fewer than half of the internalized population of unopsonized H. pylori cells are destroyed (1).…”

Molecular Basis of H2O2 Resistance Mediated by Streptococcal Dpr

“…The family members seem to be involved in aerotolerance (11), H 2 O 2 resistance (11,12,57), cold shock adaptation (58), starvation tolerance (12), iron storage (22), neutrophil activation (59,60), carbohydrate binding (61), and adhesion (62). The molecular determinants mediating these activities are largely unknown.…”

Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer

“…H pylori neutrophilactivating protein (HP-NAP), a 150-kDa iron-binding protein, is a ball-shaped dodecamer formed by fourhelix bundled subunits with its sequence similar to that of bacterioferritins and DNA binding proteins [5,6] . It has been designated as a neutrophil-activating factor because it promotes the adherence of neutrophils to endothelial cells and stimulates production of reactive oxygen species (ROS) in neutrophils by activating nicotinamide adenine dinucleotide phosphate (NADPH) oxidase in plasma membrane [7][8][9][10][11] . Satin et al [12] demonstrated that the purified recombinant HP-NAP is chemotactic for human neutrophils and monocytes, induces surface expression of β2-integrin, which mediates endothelial transmigration, adhesion and accumulation of leucocytes at the site of H pylori infection.…”