Carbohydrate digestion in ticks and a digestive α-l-fucosidase

Moreti, Rodrigo; Perrella, Natalia N.; Lopes, Adriana Rios

doi:10.1016/j.jinsphys.2013.08.008

Cited by 25 publications

(24 citation statements)

References 57 publications

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“…In Drosophila , the α‐L‐fucosidase mRNA was detected in the midgut . In addition, digestive α‐L‐fucosidase was found in the midgut or midgut glands in several other species, including Amblyomma sculptum , Nephilingis cruentata , Tityus serrulatus , Pecten maximum , and Biomphalaria glabrata . α‐L‐fucosidase from the plant pathogenic fungus F. graminearum was found actively hydrolyzing the fucose from xyloglucan .…”

Section: Discussionmentioning

confidence: 99%

Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Tang

Zhu

et al. 2019

Pest Management Science

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BACKGROUND The pine wood nematode (PWN) Bursaphelenchus xylophilus is the causal agent of pine wilt disease (PWD). This disease is a serious threat to pine forests globally. The fuca gene encodes α‐L‐fucosidase, which plays crucial roles in numerous biological and pathological processes in bacteria, fungi, plants and animals. To find promising control strategies against PWD, we investigated the expression and functions of Bxy‐fuca in B. xylophilus. RESULTS Bxy‐fuca encoding α‐L‐fucosidase is highly conserved within the deduced functional domains and key residues. It is expressed continuously across all developmental stages of B. xylophilus. mRNA in situ hybridization demonstrated that Bxy‐fuca was mainly localized in the body wall muscles and intestine. RNA interference indicated that the zygotic expression of Bxy‐fuca was indispensable for embryogenesis. The rate of B. xylophilus egg hatch was significantly decreased after Bxy‐fuca was interfered. Postembryonic silence of Bxy‐fuca resulted in a dramatic decrease in the longevity of and the total number of eggs produced by B. xylophilus. In addition, the motility of the nematode was greatly hampered with a significant drop in head thrashing frequency. CONCLUSION Bxy‐fuca plays crucial roles in development, lifespan and reproduction of B. xylophilus. Our results provide promising hints for control of PWD by blocking embryogenesis and ontogenesis, decreasing nematode fecundity, and disrupting the connection between B. xylophilus and its vector beetle by preventing nematode movement into the tracheal system. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Tang

Zhu

et al. 2019

Pest Management Science

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“…The gut of ticks also expresses enzymes that digest other blood meal constituents, such as carbohydrates and lipids (MORETI et al, 2013;KOTSYFAKIS et al, 2015;XU et al, 2016). Carbohydrase genes were identified in the digestive tract of O. mimon after the blood meal, among which chitinases and α-glycosidases were the most prevalent.…”

Section: Blood Meal Digestion and Nutrient Uptakementioning

confidence: 99%

“…The role of these enzymes within digestion has not been fully understood and there are few studies about it. However, on the other hand, the activity of carbohydrases such as N-acetyl glucosaminidase, chitinase and α-L-fucosidase on the intestinal content of Amblyomma sculptum (cited as cajennense) was recently identified and characterized, and it was suggested that digestion of carbohydrates occurs in the intestinal lumen, unlike the intracellular digestion of proteins (MORETI et al, 2013).…”

Section: Blood Meal Digestion and Nutrient Uptakementioning

confidence: 99%

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums and is also harmful to humans. Knowledge of the transcripts present in the tick gut helps in understanding the role of vital molecules in the digestion process and parasite-host relationship, while also providing information about the evolution of arthropod hematophagy. Thus, the present study aimed to know and ascertain the main molecules expressed in the gut of argasid after their blood meal, through analysis on the gut transcriptome of engorged females of O. mimon using 454-based RNA sequencing. The gut transcriptome analysis reveals several transcripts associated with hemoglobin digestion, such as serine, cysteine, aspartic proteases and metalloenzymes. The phylogenetic analysis on the peptidases confirmed that most of them are clustered with other tick genes. We recorded the presence a cathepsin O peptidase-coding transcript in ticks. The topology of the phylogenetic inferences, based on transcripts of inferred families of homologues, was similar to that of previous reports based on mitochondrial genome and nuclear rRNA sequences. We deposited 2,213 sequence of O. mimon to the public databases. Our findings may help towards better understanding of important argasid metabolic processes, such as digestion, nutrition and immunity.Keywords: Argasidae, transcriptome, gut, phylogeny, Ornithodoros mimon. ResumoOrnithodoros mimon é um carrapato argasídeo parasita de morcegos, aves e marsupiais, além de ser bastante agressivo aos humanos. O conhecimento dos transcritos presentes no intestino dos carrapatos auxilia no entendimento do papel de moléculas vitais no processo de digestão e na relação parasito-hospedeiro, além de fornecer também informações sobre a evolução dos artrópodes hematófagos. Desta maneira, o presente estudo teve como objetivo conhecer e identificar as principais moléculas expressas no intestino de uma espécie de carrapato argasídeo após o repasto sanguíneo, através de uma análise transcritômica descritiva do intestino de fêmeas ingurgitadas de O. mimon, utilizando um sequenciamento de RNA de nova geração da plataforma 454. Além de inferir a relação filogenética de carrapatos através de um conjunto de dados transcritômicos. O transcriptoma do intestino revelou diversos transcritos associados com a digestão da hemoglobina, como proteinases das classes serino, cisteína, aspártica e metalo. Registramos a presença de um transcrito de uma cisteína peptidase do tipo catepsina O em carrapatos. A inferência filogenética baseada em conjunto de dados transcritos homólogos tem uma resolução topológica similar a de outros conjuntos de dados moleculares. Foram depositados no banco de dados gênico público 2213 transcritos de O. mimon. Os achados obtidos no presente estudo podem contribuir para compreensão dos importantes processos, como digestão, nutrição e imunidade dos carrapatos da família Argasidae, além de fornecer informações sobre a filogenia da ordem Ixodida.

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“…Pasini and co‐workers have shown that α‐ l ‐fucosidase is expressed in the midgut of Drosophila (Pasini, Intra, & Pavesi, ) and digestive fucosidases have been isolated from molluscs and crustaceans (Berteau, McCort, Goasdoue, Tissot, & Daniel, ; Perrella, Cantinha, Nakano, & Lopes, ; Reglero & Cabezas, ). Our group has previously identified and characterized a digestive α‐ l ‐fucosidase from the tick Amblyomma sculptum (Moreti, Perrella, & Lopes, ). Transcriptomic and proteomic analyses of the digestive tract of the scorpion Tityus serrulatus (Fuzita et al., ) and the spider Nephilingis cruentata (Fuzita, Pinkse, Patane, Verhaert, & Lopes, ) have led to the identification of α‐ l ‐fucosidase mRNA and corresponding peptides.…”

Section: Introductionmentioning

confidence: 99%

“…Despite such evidence, the physiological roles of digestive fucosidases from Arachnida species are still unclear. These fucosidases could be involved in the removal of l ‐fucose residues from food sources for metabolic purposes, they may play a defensive role, such as the removal of l ‐fucose residues involved in the interaction of pathogenic microorganisms and ticks (Gulia‐Nuss et al., ; Moreti et al., ; Pedra et al., ; Severo, Stephens, Kotsyfakis, & Pedra, ), or they could simply act as lysosomal enzymes.…”

Section: Introductionmentioning

confidence: 99%

First characterization of fucosidases in spiders

Perrella

Fuzita

Moreti

et al. 2018

Arch Insect Biochem Physiol

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l-fucose is a constituent of glycoconjugates in different organisms. Fucosidases catalyze the removal of fucose residues, and have been correlated to different physiological and pathological processes, such as fertilization, cancer, fucosidosis, and digestion in molluscs and ticks. An α-l-fucosidase sequence was identified from the transcriptome and proteome from the midgut diverticula of the synanthropic spider Nephilingis cruentata. In this article, we describe the isolation of this α-l-fucosidase and the characterization of its activity using substrates and inhibitors demonstrating different specificities among fucosidases. The enzyme had a K of 32 and 400 μM for 4-methylumbelliferyl α-l-fucopyranoside and 4-nitrophenyl α-l-fucopyranoside, respectively; and was unable to hydrolyze fucoidan. Nephilingis cruentata α-l-fucosidase was inhibited competitively by fucose and fuconojyrimycin. The fucosidase had two distinct pH optima even in the isolated form, due to oligomerization dependent on pH, as previously described to other fucosidases. Alignment and molecular homology modeling of the protein sequence with other fucosidases indicated that the active sites and catalytic residues were different, including residues involved in acid/base catalysis. Phylogenetic analysis showed, for the first time, gene-duplication events for fucosidases in Arachnida species. All these data reveal that studies on fucosidases in organisms distinct from bacteria, fungi, and humans are important.

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Carbohydrate digestion in ticks and a digestive α-l-fucosidase

Cited by 25 publications

References 57 publications

Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

First characterization of fucosidases in spiders

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