Carbohydrate immunity in American trypanosomiasis

Travassos, Luiz R.; Almeida, Igor C.

doi:10.1007/bf00201100

Cited by 26 publications

(34 citation statements)

References 87 publications

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“…However, after successful treatment, the results of conventional serologic assays remain positive for years or even decades during the chronic phase of Chagas disease, for reasons that are not yet well understood. These reasons most likely involve recurrent, nonspecific polyclonal activation and thus nonspecific, low-titre Abs (Almeida et al 1993, 1997, Travassos & Almeida 1993, Andrade et al 1996, 2004, Rassi & Luquetti 2003). The Ab-dependent lysis of infective T. cruzi stages by patient serum is a more reliable indicator of ongoing active infection and detects lytic Abs in which the immunodominant epitopes present on the surface of living trypomasti-gotes are recognised (Travassos & Almeida 1993, Krettli 2009).…”

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confidence: 99%

Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

Izquierdo

Marques

Gállego

et al. 2013

Mem. Inst. Oswaldo Cruz

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The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI) = 96-100} and specific (98.3%; 95% CI = 90.7-99.7), with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.

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confidence: 99%

Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

Izquierdo

Marques

Gállego

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…Many T. cruzi antigens are involved in chronic chagasic cardiopathy, such as FL-160-1, a surface protein associated with the 160 kDa flagellum which has an epitope that cross-reacts with the nervous tissue, and Gp85, a surface glycoprotein which reacts with chronic chagasic sera as well as with common glycolipid antigens present both in the mammalian nervous system and in T. cruzi trypomastigote forms (7,13,21,22). Experiments using molecular cloning have revealed that chronic chagasic cardiopathy control.…”

Section: Discussionmentioning

confidence: 99%

Presence of autoantibodies against HeLa small nuclear ribonucleoproteins in chagasic and non-chagasic cardiac patients

Bosetto

Peixoto

Castro

et al. 2004

Braz J Med Biol Res

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We detected anti-human small nuclear ribonucleoprotein (snRNP) autoantibodies in chagasic patients by different immunological methods using HeLa snRNPs. ELISA with Trypanosoma cruzi total lysate antigen or HeLa human U small nuclear ribonucleoproteins (UsnRNPs) followed by incubation with sera from chronic chagasic and nonchagasic cardiac patients was used to screen and compare serum reactivity. Western blot analysis using a T. cruzi total cell extract was also performed in order to select some sera for Western blot and immunoprecipitation assays with HeLa nuclear extract. ELISA showed that 73 and 95% of chronic chagasic sera reacted with HeLa UsnRNPs and T. cruzi antigens, respectively. The Western blot assay demonstrated that non-chagasic cardiac sera reacted with high molecular weight proteins present in T. cruzi total extract, probably explaining the 31% reactivity found by ELISA. However, these sera reacted weakly with HeLa UsnRNPs, in contrast to the chagasic sera, which showed autoantibodies with human Sm (from Stefanie Smith, the first patient in whom this activity was identified) proteins (B/B', D1, D2, D3, E, F, and G UsnRNP). Immunoprecipitation reactions using HeLa nuclear extracts confirmed the reactivity of chagasic sera and human UsnRNA/RNPs, while the other sera reacted weakly only with U1snRNP. These findings agree with previously reported data, thus supporting the idea of the presence of autoimmune antibodies in chagasic patients. Interestingly, non-chagasic cardiac sera also showed reactivity with T. cruzi antigen and HeLa UsnRNPs, which suggests that individuals with heart disease of unknown etiology may develop autoimmune antibodies at any time. The detection of UsnRNP autoantibodies in chagasic patients might contribute to our understanding of how they develop upon initial T. cruzi infection.

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“…Antigal antibodies from both chagasic and normal sera bind to Galα(1-3)Galβ(1-4)GlcNAc -Synsorb or melibiose (Galα(1-6)Glc) -agarose and react with Galα(1-3)Gal epitopes of mouse laminin (LAM) (5)(6)(7)(8)(9)(10). However, only antigal from patients with acute or chronic Chagas' disease lyse trypomastigotes through different pathways (2,3,(5)(6)(7)(8)(9)11). Besides contributing to the reduction of parasitemia, chagasic antigal may play a role in the immunopathology of human Chagas' disease (1,9,11,12).…”

Section: Introductionmentioning

confidence: 99%

Reactivity of chagasic antigal antibodies with noninfected cells treated withTrypanosoma cruzi secreted/excreted antigens

Yokoyama-Yasunaka

Piazza

Umezawa³

et al. 1998

J. Clin. Lab. Anal.

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Carbohydrate immunity in American trypanosomiasis

Cited by 26 publications

References 87 publications

Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

Presence of autoantibodies against HeLa small nuclear ribonucleoproteins in chagasic and non-chagasic cardiac patients

Reactivity of chagasic antigal antibodies with noninfected cells treated withTrypanosoma cruzi secreted/excreted antigens

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