Carbohydrate‐induced modulation of cell membrane. VIII. Agglutination with mammalian lectin galectin‐1 increases osmofragility and membrane fluidity of trypsinized erythrocytes

Abstract

Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growthregulatory family known to interact for example with ganglioside GM 1 and also the hydrophobic tail of oncogenic H-Ras. Assays on membrane fluidity and osmofragility detect galectin-1's capacity to increase the parameters. Moreover, it increases susceptibility of erythrocytes to radical damage. These observations indicate the potential of this endogenous lectin to affect membrane properties beyond the immediate interaction with cell surface epitopes.

Disease-associated glycosylated molecular variants of human C-reactive protein activate complement-mediated hemolysis of erythrocytes in tuberculosis and Indian visceral leishmaniasis

“…This membrane destabilization could be due to the lectin-like behaviour of CRP [16,45]. Thus CRP when added exogenously will cross-link with the membrane surface receptors leading to lateral molecular reorganizations in the plane of the bilayer [63][64][65].…”

Evaluation of consequences of the treatment of blood with extracts of Hypericum perforatum and Mentha crispa

“…Besides these analyses, the study of osmotic fragility of RBC is also very relevant (Gupta et al, 2006;Panis and Souza, 2005).…”

“…Ionescu-Zanetti et al (2005) studied changes in the morphology of individual cells during membrane porationdriven hemolysis providing an explanation for increased RBC-OF in patients who have spherocytosis. Gupta et al (2006) observed the potential of endogenous lectin to affect membrane properties such as OF beyond the immediate interaction with cell surface epitopes.…”

“…Blood samples have been used in some assays to evaluate the action of various chemical and physical agents (Ionescu-Zanetti et al, 2005;Gupta et al, 2006). Qualitative (morphology) and quantitative (morphometry) analysis of red blood cells (RBC) is an important method of clinical and laboratory investigations and has contributed to evaluate possible alterations in the area, shape, volume, and perimeter-to-area ratio of RBC cellular structure, with findings relevant to various pathological disorders (Engstrom and Lofvenberg, 1998;Vaya et al, 2005), and are due to the presence of plant extracts, identifying possible effects (Oliveira et al, 2003a(Oliveira et al, , 2005Lima-Filho et al, 2004).…”

“…Qualitative (morphology) and quantitative (morphometry) analysis of red blood cells (RBC) is an important method of clinical and laboratory investigations and has contributed to evaluate possible alterations in the area, shape, volume, and perimeter-to-area ratio of RBC cellular structure, with findings relevant to various pathological disorders (Engstrom and Lofvenberg, 1998;Vaya et al, 2005), and are due to the presence of plant extracts, identifying possible effects (Oliveira et al, 2003a(Oliveira et al, , 2005Lima-Filho et al, 2004). Studies on osmotic fragility of blood cells have been used as a diagnostic tool for blood disorders (Ionescu-Zanetti et al, 2005) and to enable assessment of important properties of various agents (Gupta et al, 2006).…”