Carbohydrate Metabolism in Drosophila: Reliance on the Disaccharide Trehalose

Reyes-DelaTorre, Alejandro; Peña-Rangel, María Teresa; Riesgo-Escovar, Juan R.

doi:10.5772/50633

Cited by 23 publications

(23 citation statements)

References 69 publications

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“…Trehalose is a very crucial component of insect physiology (Reyes-DelaTorre et al 2012). In our study, exogenous feeding of trehalose dramatically increased the in vivo trehalose pools and also enhanced larval desiccation tolerance.…”

Section: Resultssupporting

confidence: 56%

“…One of the common mechanisms of desiccation tolerance is the ability of organisms to synthesise compatible solutes like proline, glycine betaine, umbelliferose, trehalose, mannitol, sorbitol, glycerol and LEA proteins (Tunnacliffe and Lapinski 2003). Among these biomolecules, trehalose is well known as a stress protectant (Reyes-DelaTorre et al 2012) and is also drawing attention for its novel roles in nutrition-associated behaviour in insects (Liua et al 2013). Trehalose is extensively found in bacteria, fungi, plants and invertebrate animals but is absent in vertebrates (Shukla et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of dTps1 in Drosophila melanogaster larvae confirms involvement of trehalose in redox regulation following desiccation

Thorat

Mani

Thangaraj

et al. 2016

Cell Stress and Chaperones

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As a survival strategy to environmental water deficits, desiccation-tolerant organisms are commonly known for their ability to recruit stress-protective biomolecules such as trehalose. We have previously reported the pivotal role of trehalose in larval desiccation tolerance in Drosophila melanogaster. Trehalose has emerged as a versatile molecule, serving mainly as energy source in insects and also being a stress protectant. While several recent reports have revealed the unconventional role of trehalose in scavenging reactive oxygen species in yeast and plants, this aspect has not received much attention in animals. We examined the status of desiccation-induced generation of reactive oxygen species in D. melanogaster larvae and the possible involvement of trehalose in ameliorating the harmful consequences thereof. Insect trehalose synthesis is governed by the enzyme trehalose 6-phosphate synthase 1 (TPS1). Using the ubiquitous da-GAL4-driven expression of the dTps1-RNAi transgene, we generated dTps1-downregulated Drosophila larvae possessing depleted levels of dTps1 transcripts. This resulted in the inability of the larvae for trehalose synthesis, thereby allowing us to elucidate the significance of trehalose in the regulation of desiccation-responsive redox homeostasis. Furthermore, the results from molecular genetics studies, biochemical assays, electron spin resonance analyses and a simple, non-invasive method of whole larval live imaging suggested that trehalose in collaboration with superoxide dismutase (SOD) is involved in the maintenance of redox state in D. melanogaster.

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Section: Resultssupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Downregulation of dTps1 in Drosophila melanogaster larvae confirms involvement of trehalose in redox regulation following desiccation

Thorat

Mani

Thangaraj

et al. 2016

Cell Stress and Chaperones

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“…5E). As trehalose‐6‐phosphate synthase (TPS) and TRET are critical to trehalose synthesis and transport (Reyes‐DelaTorre, Peña‐Rangel, and Riesgo‐Escovar, ), we further investigated the effects of AKHR RNAi on the expression of TPS (GenBank: EU131894) and TRET (GenBank: AY627302). After AKHR knockdown, TRET transcripts declined by 55.6% in the fat body, whereas TPS mRNA levels were not significantly altered but showed a decreasing trend (Fig.…”

Section: Resultsmentioning

confidence: 99%

Rhythmic change of adipokinetic hormones diurnally regulates locust vitellogenesis and egg development

Zheng

Chen

Liu

et al. 2020

Insect Molecular Biology

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Adipokinetic hormones (AKHs), the neurohormones synthesized in the insect corpora cardiaca are known to mobilize lipids and carbohydrates for energyconsuming activities including reproduction. However, both inhibitory and stimulatory effects of AKHs on insect reproduction have been reported, and the underlying mechanisms remain elusive. Using the migratory locust, Locusta migratoria, as a model system, we report here that AKHs are expressed in response to rhythmic diel change, and AKH III expression increases markedly at photophase. Diurnal injection of AKH III but not AKH I or AKH II in adult females stimulates vitellogenesis and egg development. In contrast, AKH treatment at scotophase represses female reproduction. RNA interference-mediated knockdown of AKH receptor (AKHR) results in significantly reduced vitellogenin (Vg) expression in the fat body at photophase along with reduced Vg deposition in the ovary. AKHR knockdown also leads to decreased expression of Brummer, triacylglycerol lipase and trehalose transporter, accompanied by suppressed mobilization of triacylglycerol and trehalose. We propose that in addition to stimulating Vg expression at photophase, AKH/AKHR signalling is likely to regulate ovarian uptake of Vg via triacylglycerol mobilization and trehalose homeostasis. This study provides new insights into the understanding of AKH/AKHR signalling in the regulation of insect reproduction.

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“…Trehalose is considered the blood sugar of insects [ 31 ], but we found glucose to be the most dominant sugar in the haemolymph [ 20 ] and whole adult D . citri [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

Metabolic alterations in the nymphal instars of Diaphorina citri induced by Candidatus Liberibacter asiaticus, the putative pathogen of huanglongbing

Killiny

Jones

2018

PLoS ONE

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Currently, huanglongbing is the most damaging disease of citrus causing huge economic losses. The disease is caused by the Gram-negative bacterium Candidatus Liberibacter asiaticus (CLas). The pathogen is transmitted in a persistent propagative circulative manner within its vector, the Asian citrus psyllid, Diaphorina citri. Exploring the metabolic alteration in the vector may lead to a better understanding of the nutritional needs of CLas and to designing an artificial medium for culturing the pathogen. It has been shown that the nymphal stages have a greater role in transmission mainly because they feed on plants more actively than adults. In this study, we carried out an untargeted comparative metabolomic analysis for healthy and CLas-infected 4th / 5th instar nymphs. The metabolic analysis was performed using trimethylsilylation and methyl chloroformate derivatization followed by Gas Chromatography-Mass Spectrometry (GC-MS). Overall, the changes in the nymph metabolism due to the infection with CLas were more pronounced than in adults, as we previously published. Nymphs reared on CLas-infected Valencia sweet orange were higher in many metabolites, mainly those of the TCA cycle, C16 and C18 fatty acids, glucose, sucrose, L-proline, L-serine, pyroglutamic acid, saccharic acid, threonic acid and myo-inositol than those reared on healthy plants. In contrast, CLas-infected nymphs were lower in putrescine, glycine, L -phenylalanine, L -tyrosine, L -valine, and chiro-inositol. The information provided from this study may contribute in acceleration of the availability of CLas in culture and consequent screening of antibacterial compounds to discover a definitive solution for huanglongbing.

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Carbohydrate Metabolism in Drosophila: Reliance on the Disaccharide Trehalose

Cited by 23 publications

References 69 publications

Downregulation of dTps1 in Drosophila melanogaster larvae confirms involvement of trehalose in redox regulation following desiccation

Downregulation of dTps1 in Drosophila melanogaster larvae confirms involvement of trehalose in redox regulation following desiccation

Rhythmic change of adipokinetic hormones diurnally regulates locust vitellogenesis and egg development

Metabolic alterations in the nymphal instars of Diaphorina citri induced by Candidatus Liberibacter asiaticus, the putative pathogen of huanglongbing

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