Carbohydrate Microarrays

Mellet, Carmen Ortiz; Fernández, José Manuel Garcı́a

doi:10.1002/1439-7633(20020902)3:9<819::aid-cbic819>3.0.co;2-z

Cited by 62 publications

(45 citation statements)

References 9 publications

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“…A new emerging and powerful glycomics tools such as biochips/arrays, high throughput mass spectroscopy, liquid spectroscopy and nuclear magnetic resonance are behind huge progress in the field [50,51,52,53,54,55,56,57]. Biochips/arrays are clearly benefiting from more mature and successful technology of DNA biochips [58,59,60,61,62, 63,64,65,66,67]. The advantage of using biochips is a minute consumption of biorecognition elements printed at high density with high throughput of assays.…”

Section: Glycomicsmentioning

confidence: 99%

Electrochemical lectin based biosensors as a label-free tool in glycomics

et al. 2012

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Glycans and other saccharide moieties attached to proteins and lipids, or present on the surface of a cell, are actively involved in numerous physiological or pathological processes. Their structural flexibility (that is based on the formation of various kinds of linkages between saccharides) is making glycans superb "identity cards". In fact, glycans can form more "words" or "codes" (i.e., unique sequences) from the same number of "letters" (building blocks) than DNA or proteins. Glycans are physicochemically similar and it is not a trivial task to identify their sequence, or - even more challenging - to link a given glycan to a particular physiological or pathological process. Lectins can recognise differences in glycan compositions even in their bound state and therefore are most useful tools in the task to decipher the "glycocode". Thus, lectin-based biosensors working in a label-free mode can effectively complement the current weaponry of analytical tools in glycomics. This review gives an introduction into the area of glycomics and then focuses on the design, analytical performance, and practical utility of lectin-based electrochemical label-free biosensors for the detection of isolated glycoproteins or intact cells.

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Section: Glycomicsmentioning

confidence: 99%

Electrochemical lectin based biosensors as a label-free tool in glycomics

et al. 2012

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“…Small molecule arrays have aided the rapid identification of compounds that bind to proteins, thus enabling chemical genetics experiments 5–7. More recently, carbohydrate arrays to probe carbohydrate–protein interactions have been described 8–10. The potential to screen a large number of events in parallel with minuscule amounts of both ligand and analyte has resulted in the widespread use of this technology.…”

Section: Introductionmentioning

confidence: 99%

Aminoglycoside Microarrays To Explore Interactions of Antibiotics with RNAs and Proteins

Disney

Seeberger

2004

Chemistry A European J

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RNA is an important target for drug discovery efforts. Several clinically used aminoglycoside antibiotics bind to bacterial rRNA and inhibit protein synthesis. Aminoglycosides, however, are losing efficacy due to their inherent toxicity and the increase in antibiotic resistance. Targeting of other RNAs is also becoming more attractive thanks to the discovery of new potential RNA drug targets through genome sequencing and biochemical efforts. Identification of new compounds that target RNA is therefore urgent, and we report here on the development of rapid screening methods to probe binding of low molecular weight ligands to proteins and RNAs. A series of aminoglycosides has been immobilized onto glass microscope slides, and binding to proteins and RNAs has been detected by fluorescence. Construction and analysis of the arrays is completed by standard DNA genechip technology. Binding of immobilized aminoglycosides to proteins that are models for study of aminoglycoside toxicity (DNA polymerase and phospholipase C), small RNA oligonucleotide mimics of aminoglycoside binding sites in the ribosome (rRNA A-site mimics), and a large (approximately 400 nucleotide) group I ribozyme RNA is detected. The ability to screen large RNAs alleviates many complications associated with binding experiments that use isolated truncated regions from larger RNAs. These studies lay the foundation for rapid identification of small organic ligands from combinatorial libraries that exhibit strong and selective RNA binding while displaying decreased affinity to toxicity-causing proteins.

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“…21 1.2 Spotting of pre-synthesized small molecules and peptides Over the last few years, small molecule planar microarrays have become an important tool for high-throughput experimentation in biological research. 10,26 Methods for immobilization of small molecules on a planar surface have been recently reviewed. 27 Except for the SPOT array, in which some heterocyclic libraries can be synthesized in situ, most other planar small-molecule microarrays require pre-synthesis of the small molecule compounds with an appropriate functional group for subsequent ligation.…”

Section: In Situ Synthesis Of Peptide and Non-peptide Microarraysmentioning

confidence: 99%