“…The ability to obtain such high ethanol yields in 24-48 hr under production conditions, would require careful control of the S. cerevisiae metabolic regulatory factors which are mediated, in part, by cell viability (9,10), alcohol toxicity (11-13), transport activity (14,15), and substrate and osmotic repression effects (1)(2)(3)16,17). In the high corn fermentation, the critical mediating conditions appear to involve carbohydrate and osmotic effects, so it is important to understand these regulatory phenomena in order to develop a viable process.…”