Carbohydrate specificity of theEscherichia coli P-pilus papG protein is mediated by its N-terminal part

Hansson, Lennart; Wallbrandt, Pia; Andersson, Jan-Olof; Byström, Mona; Bäckman, Assar; Carlstein, Annica; Enquist, Kerstin; Lönn, Hans; Otter, Charlotta; Strömqvist, Mats

doi:10.1016/0304-4165(95)00028-a

Cited by 19 publications

(21 citation statements)

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“…1A) in gels containing casein showed one strong proteolytic band with apparent molecular mass around 25 kDa and one weaker component with slightly lower mobility. These two bands correspond to unglycosylated and glycosylated SCCE, respectively (9). In addition, a zone of proteolysis, apparently consisting of one stronger band with molecular mass around 30 kDa, and two weaker bands, one with slightly higher mobility and one with slightly lower mobility than the major band, were seen.…”

Section: Resultsmentioning

confidence: 92%

“…Previous studies have shown that high expression of SCCE is found in the skin only (9). On the protein level, this enzyme has so far been detected only in squamous eptithelia undergoing cornification (11,22,26,27).…”

Section: Discussionmentioning

confidence: 97%

“…Healthy human skin was obtained from plastic surgery and prepared as described (9). HaCat-cells (a spontaneously immortalized human keratinocyte line (16)) was a generous gift from Dr. N. E. Fusenig (Heidelberg, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…There is evidence that one of the enzymes involved may be stratum corneum chymotryptic enzyme (SCCE) 1 (6 -9). This serine protease with chymotrypsin-like primary substrate specificity (10), which has been cloned and expressed in mammalian cells (9), has enzymatic properties (6) and a tissue localization (11)(12) compatible with it being responsible, at least partially, for the degradation of intercellular parts of desmosomes, a process that is a prerequisite for desquamation.…”

mentioning

confidence: 99%

“…One such piece of evidence is that SCCE is formed as an inactive precursor with a seven-amino acid residue-long propeptide. The propeptide can be removed in vitro by means of tryptic cleavage of a Lys-Ile bond, yielding proteolytically active SCCE (9). In the stratum corneum, the SCCE precursor constitutes a significant fraction of the total SCCE protein present.…”

mentioning

confidence: 99%

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Purification, Molecular Cloning, and Expression of a Human Stratum Corneum Trypsin-like Serine Protease with Possible Function in Desquamation

Brattsand

Egelrud

1999

Journal of Biological Chemistry

196

173

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A new human 33-kDa serine protease was purified from human epidermis, and its cDNA was cloned from a keratinocyte library, from mRNA from a human keratinocyte line (HaCat) and from mRNA from human skin. Polyclonal antibodies specific for the new protein detected three groups of proteins in partially purified extracts of cornified eptihelium of human plantar skin. The three components are proposed to correspond to proenzyme, active enzyme, and proteolytically modified active enzyme. After N-deglycosylation, there was a decrease in apparent molecular mass of all detected components. Expression of the cloned cDNA in a eukaryotic virus-derived system yielded a recombinant protein that could be converted to an active protease by treatment with trypsin. Polymerase chain reaction analyses of cDNA from a number of human tissues showed high expression of the new enzyme in the skin and low expression in brain, placenta, and kidney. Homology searches yielded the highest score for porcine enamel matrix protease (55% amino acid sequence homology). High scores were also obtained for human and mouse neuropsin and for human stratum corneum chymotryptic enzyme. The function of this new protease, tentatively named stratum corneum tryptic enzyme, may be related to stratum corneum turnover and desquamation in the epidermis.

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Section: Resultsmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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