Carbohydrate Utilization by the Soil Amoeba <i>Hartmannella castellanii</i>

Adam, Katherine M. G.; Blewett, David A.

doi:10.1111/j.1550-7408.1967.tb01998.x

Cited by 14 publications

(5 citation statements)

References 10 publications

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“…* Present Address: Parasitology Section, Center for Disease Control, Atlanta, Georgia 30333. irregular outer layer, and placed in the genus Glaeseria the amebae with nuclear division in the cyst. Unfortunately, Volkonsky included many inadequately described amebae in his genus Acanthamoeba (1,43). Singh (59) and Singh & Das (60), after making a detailed study of several strains and species of small amebae, concluded that form, locomotion, and the appearance of the cyst cannot be used as diagnostic characters in classifying amebae.…”

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Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to *Acanthamoeba**

Visvesvara¹,

Balamuth²

1975

The Journal of Protozoology

146

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Comparative studies were conducted on the structure, nutrition, protein composition, immunology, and effect on cell cultures of Acanthamoeba sp. (Lilly A-1 strain), A. castellanii (Singh and Neff strains), A. astronyxis, A. comandoni, A. polyphaga, A. terricola, Hartmannella vermiformis, and Naegleria gruberi. Lilly A-1 strain of Acanthamoeba received special attention owing to its pathogenicity for experimental animals. Distinct differences were noted in structure, nutrition, and antigenic composition of Acanthamoeba spp. and Hartmannella, and it was concluded that their recognition as separate genera is justified. With the exception of A. terricola, all species of Acanthamoeba could be differentiated by cyst structure. Cysts of A. terricola closely resembled those of A. castellanii Singh strain, and close antigenic relationships between these 2 species were demonstrated by gel diffusion and immunoelectrophoresis (IEP); it was concluded that the 2 amebae belong in the same species. The pathogenic Acanthamoeba sp, Lilly strain differed from the nonpathogenic A. castellanii Singh strain (a) cyst structure; (b) protein distribution patterns (on disc electrophoresis);(c)soluble and particulate antigens (on gel diffusion, IEP, complement fixation, and immobilization tests); (d) capacity to induce cell-free plaques and other cytopathic effect (CPE) in mammalian monlayer cell cultures; (e) elimination of a phospholipase, responsible for some of the CPE, into the culture medium. Acanthamoeba sp. Lilly strain, which liberated more phospholipase, produced more CPE. Acanthamoeba sp. Lilly strain differed also from other species of this genus in cyst structure and antigenic composition. It was concluded, therefore, that, following the recommendation of Singh & Das, it ought to be placed in a separate species, Acanthamoeba culbertsoni.

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confidence: 99%

Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to *Acanthamoeba**

Visvesvara¹,

Balamuth²

1975

The Journal of Protozoology

146

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“…The general nature of carbohydrate metabolism in A. castellanii has been partially elucidated (1,7,14,18,24,32,34). However, relatively few detailed enzymological and radioisotopic studies of carbohydrate metabolism in this protozoan have been published.…”

Section: Introduction Materials and Methodsmentioning

confidence: 99%

“…Discussion A variety of evidence indicates that the Embden-Meyerhof pathway is functional in A. castellanii. Glucose or another sugar is required as a carbon source for growth, and glycogen serves as the intracellular polysaccharide reserve (1,4,24,32,36). Incubation with '4C-sucrose results in the formation of '4C02 and radioactive cell constituents (I).…”

Section: C-glucose Degradation To 14c02mentioning

confidence: 99%

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Carbohydrate metabolism in Acanthamoeba castellanii. 1. The activity of key enzymes and [¹⁴C]-glucose metabolism

Prescott¹,

Hoyme²,

Crockett³

et al. 1973

Can. J. Microbiol.

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1973. Carbohydrate metabolism in Acanthamoeba castellanii. 1. The activity of key enzyrnes and ['4C]-glucose metabolism. Can. J. Microbiol. 19: 1131-1136.The specific activities of a number of the key enzyrnes involved in carbohydrate metabolism in Acantha1noeba castellanii (Neff clone 1-12) have been determined. The following Embden-Meyerhof and pentose phosphate pathway enzymes were present: glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, hexokinase, phosphofructokinase, hexose diphosphatase, aldolase, glyceraldehydephosphate dehydrogenase, pyruvate kinase, and pyruvate-phosphate dikinase. The following tricarboxylic acid cycle enzyrnes were also found: citrate synthase, aconitase, isocitrate dehydrogenase, succinate dehydrogenase, fumarate hydratase, and malate dehydrogenase. The degradation of glu~ose-U-'~C to 14C02 was examined. Aerobic 14C02 production from glu~ose-U-'~C was 3.4-fold greater than anaerobic production. The data provide further evidence that the Embden-Meyerhof, pentose phosphate, and tricarboxylic acid cycle pathways are probably functional in A. castellanii. PRESCOTT, L. M., H. E. HOYME, D. CROCKETT et E. HUI. 1973. Carbohydrate metabolism in Acanthamoeba castellatzii. 1. The activity of key enzyrnes and [14C]-glucose metabolism. Can. J. Microbiol.19: 1131-1136. Nous avons determine les activitks spkcifiques de plusieurs enzyrnes clees impliquies dans le metabolisme des carbohydrates chez Acanthatnoeba castellanii (Neff clone 1-12). Dans les chemins metaboliques d'Embden-Meyerhof et du phosphate pentose, les enzyrnes suivantes sont presentes: la dehydrogenase glucose-6-phosphate, la dehydrogenase 6-phosphogluconate, l'hexokinase, la phosphofructokinase, la diphosphatase hexose, l'aldolase, la dehydrogenase glyceraldehyde phosphate, la kinase pyruvate, et la dikinase pyruvate-phosphate. Dans le cycle de l'acide tricarboxylique, les enzyrnes suivantes sont aussi trouvees: la synthase citrate, l'aconitase, la dehydrogenase isocitrate, la dehydrogenase succinate, l'hydratase fumarate et la dehydrogenase malate. La degradation du g l~c o s e -U -~~C en 14C02 fut examinee. La production aerobique du 14C02 a partir du glucose-U-14C est 3.4 fois superieure que la production anaerobique. Les donnees fournissent d'autres evidences que les chemins metaboliques d'Embden-Meyerhof, du phosphate pentose et du cycle de l'acide tricarboxylique sont probablement fonctionnels chez A. castellanii.praduit par le journal]

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“…MALL, free-living amoebae of the genus Acanthamoeba S (family Acanthamoebidae) have attracted many investigators since Castellani (6) described the first species, not only because of the cosmopolitan distribution of the genus but with the recent recognition that several strains are pathogenic to humans and animals. Defined media have been described for A. castellanii (1)(2)(3)(4)(5)8): however, information on the requirements of other species of this genus is limited. Investigations of the growth factors of other Acantharnoehu spp., particularly those which are pathogenic seems warranted.…”

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Defined Minimal Growth Medium for Acanthamoeba polyphaga

Ingalls

Brent

1983

The Journal of Protozoology

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Carbohydrate Utilization by the Soil Amoeba Hartmannella castellanii

Cited by 14 publications

References 10 publications

Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to *Acanthamoeba**

Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to *Acanthamoeba**

Carbohydrate metabolism in Acanthamoeba castellanii. 1. The activity of key enzymes and [¹⁴C]-glucose metabolism

Defined Minimal Growth Medium for Acanthamoeba polyphaga

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Carbohydrate Utilization by the Soil Amoeba Hartmannella castellanii

Cited by 14 publications

References 10 publications

Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to Acanthamoeba*

Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to Acanthamoeba*

Carbohydrate metabolism in Acanthamoeba castellanii. 1. The activity of key enzymes and [14C]-glucose metabolism

Defined Minimal Growth Medium for Acanthamoeba polyphaga

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Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to *Acanthamoeba**

Comparative Studies on Related Free‐Living and Pathogenic Amebae With Special Reference to *Acanthamoeba**

Carbohydrate metabolism in Acanthamoeba castellanii. 1. The activity of key enzymes and [¹⁴C]-glucose metabolism