Carbohydrates from Santa Barbara Basin sediments: Gas chromatographic-mass spectrometric analysis of trimethylsilyl derivatives

Analytical methods for the determination of sugars in marine samples: A historical perspective and future directions

“…This high number of peaks indicates that internal standards (adonitol and sorbitol are commonly employed) are critical in obtaining accurate results. GC-FID has been widely employed for analysis of marine samples, including suspended (Hedges et al 1994;Cotrim da Cuhna et al 2001;D'Souza and Bhosle 2001) and sinking POM (Cowie and Hedges 1984b;Tanoue and Handa 1987;Hernes et al 1996;Bergamaschi et al 1999;Ogier et al 2001), UDOM (Hedges et al 1994;McCarthy et al 1996;Aluwihare et al 1997;Benner and Opsahl 2001;Repeta et al 2002), and sediments (Modzeleski et al 1971;Klok et al 1984;Bergamaschi et al 1999;Ogier et al 2001). Because of its poor detection limit (100-150 nM) compared to modern chromatographic techniques (HPAEC-PAD), data on concentrations of dissolved free monosaccharides are not reported in literature.…”

“…A variety of compounds such as trimethylsilyl ethers (Modzeleski et al 1971;Cowie andHedges 1984a, 1984b;Li and Andrews 1986;Hernes et al 1996), trifluocacetate esters (Eklund et al 1977), and acetyl derivatives (Klok et al 1984;Sakugawa and Handa 1985;Bhosle et al 1992;Tanoue and Handa 1987) have been used to form volatile sugar derivatives. The precision of the GC-FID method ranges from 10% to 20% depending on the sugar concentration (Cowie and Hedges 1984a;McCarthy et al 1996;Sigleo 1996;D'Souza and Bhosle 2001;Ogier et al 2001).…”

Aspects of the biogeochemistry of carbohydrates in aquatic environments

“…In Figure4a analyses of the same Santa Barbara Basin sediment sample (" 50 years") were performed in both laboratories with the identical extraction procedure (Modzeleski et aL., 1971). The linear correlation be- Caption on following page.…”

“…exactly., Table i Figure 4 shows the results of an intercalibration experiment between my liquid chromatography system and the gas chromatography-mass spectrometr system described and operated by Modzeleski et~. (1971).…”

“…Prior to the study of Modzeleski et al (1971) provèd difficult because of the variations in extraction techniques and means of identification. For example, free sugars were extracted in either boiling or cold 70% ethanol for 5 minutes to eight hours; in a few instances distilled water alone wa s used as the extractant (Vallentyne and Bidwell, 1956;Whittaker and Vallentyne, 1957;Swain, 1969).…”