Carbohydrates in protein. 2. The hexose, hexosamine, acetyl and amide-nitrogen content of hen's-egg albumin

Johansen, Patricia G.; Marshall, Ruth; Neuberger, A.

doi:10.1042/bj0770239

Cited by 145 publications

(55 citation statements)

References 39 publications

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“…Hexosamine contents of granulation tissues were estimated with minor modifications (Johansen et al, 1960). The diluted solution was mixed with acetylacetone solution and heated to 96 • C for 40 min.…”

Section: Estimation Of Hydroxyproline and Hexosaminementioning

confidence: 99%

In vivo antioxidative property, antimicrobial and wound healing activity of flower extracts of Pyrostegia venusta (Ker Gawl) Miers

Roy

Amdekar

Kumar

et al. 2012

Journal of Ethnopharmacology

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Section: Estimation Of Hydroxyproline and Hexosaminementioning

confidence: 99%

In vivo antioxidative property, antimicrobial and wound healing activity of flower extracts of Pyrostegia venusta (Ker Gawl) Miers

Roy

Amdekar

Kumar

et al. 2012

Journal of Ethnopharmacology

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“…hydrolysis of chitin recoveries of pure glucosamine hydrochloride were similar, indicating that the hydrolysis of the chitin was probably complete. Ogston and Stanier (1950) heated glucosamine hydrochloride with 4N hydrochloric acid in a sealed tube in boiling water for 8 hr and obtained a rather variable recovery of glucosamine (72-96%) while Haab and Anastassiadis (1961) reported that 4N hydrochloric acid at 100°C in sealed tubes caused no decomposition of glucosamine in 24 hr (see also Johansen, Marshall, and Neuberger 1960). Purchase and Braun (1955) also reported a 56-66% yield of glucosamine hydrochloride from chitin.…”

Section: Studies On Chitin V 533mentioning

confidence: 99%

Studies on Chitin V. The Action of Mineral Acids on Chitin

Hackman¹

1962

Aust. Jnl. Of Bio. Sci.

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SummaryChitin undergoes rapid and extensive degradation at 20°C when dissolved in ION hydrochloric acid, 21N sulphuric acid, or 85% phosphoric acid. Most of the degradation occurs in the first few minutes after the chitin is dissolved and the products formed are oligosaccharides (which are to some extent deacetylated) and N·acetyl-D.glucosamine. Glucosamine was not found except possibly in the smallest traces. "Colloidal" chitin, prepared by precipitation of chitin from solution in cold 21N sulphuric acid, has a very much shorter chain length than the chitin from which it was prepared. Chitin is more stable in dilute (2N) hydrochloric acid. At 25°C only a negligible amount of hydrolysis occurs in 24 hr but at 100°C significant amounts of glucosamine and oligosaccharides are formed. For complete hydrolysis of chitin with 5· 7N hydrochloric acid it is necessary to heat at 100°C for several hours. The preparation of chitin as 'a substrate for the enzyme chitinase is discussed in the light of these findings.

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“…[1] The usual method for the determination of glucosamine and galactosamine depends on their separation by columns of sulfonic acid resin; their assay is performed by one of many modifications of the Morgan-Elson method. N-acetyl glucosamine can be determined by the Morgan-Elson (ME) reaction; on heating n-acetyl glucosamine in alkaline solution, furan derivatives are formed which subsequently react with p-dimethylaminobenzaldehyde in strong acid to form a red dye.…”

Section: Introductionmentioning

confidence: 99%

Optimum Pyrolytic Conditions for Furan Derivative Formation From Polyamino Sugars

Badadani

Shetty

2009

Preparative Biochemistry and Biotechnology

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Furan derivatives were prepared by hydrolysis of glucosamine derived from polyamino sugars assisted with pyrolysis in alkaline condition. Pyrolysis was carried out with autoclaving at 15 psi/121 degrees C/1 hour. The reaction was monitored by observing spectral characteristics at lambda(max) 545 nm with p-dimethylaminobenzaldehyde. Alkaline hydrolysis favors the formation of furan derivatives in N-acetylglucosamine, glucosamine, hyaluronic acid, but not in dermatan sulfate and chondroitin sulfate. These results indicate -OH group orientation in the cis position at C(4) of the Haworth projection formula of hexosamines may be playing a role in the formation of furans. This can be a useful method for estimation of the glucosamine in glycoprotein and urine.

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Carbohydrates in protein. 2. The hexose, hexosamine, acetyl and amide-nitrogen content of hen's-egg albumin

Cited by 145 publications

References 39 publications

In vivo antioxidative property, antimicrobial and wound healing activity of flower extracts of Pyrostegia venusta (Ker Gawl) Miers

In vivo antioxidative property, antimicrobial and wound healing activity of flower extracts of Pyrostegia venusta (Ker Gawl) Miers

Studies on Chitin V. The Action of Mineral Acids on Chitin

Optimum Pyrolytic Conditions for Furan Derivative Formation From Polyamino Sugars

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