Carbohydrates of the otolith organ in the rainbow trout Oncorhynchus mykiss detected by lectins

Takagi, Yasuaki; Ishida, Kou; Matsumura, Yasuo

doi:10.1046/j.1444-2906.2000.00149.x

Cited by 10 publications

(5 citation statements)

References 37 publications

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“…Other experiments suggest that Ca 2+ ions can also move into the endolymph via passive paracellular diffusion process (Tohse and Mugiya, 2001;Payan et al, 2002;Cruz et al, 2009). The endolymph is supersaturated in CO3 2ions relative to the blood plasma as a result of active transport across the endolymphatic epithelium (Takagi, 2002), promoting the precipitation of CaCO3 crystals (Payan et al, 1997;Takagi et al, 2000). Elevated concentrations of HCO3in the endolymphatic epithelium are achieved by intracellular activity of carbonic anhydrase, which catalyzes the CO2 + H2O → HCO3 -+ H + reaction (Mayer-Gostan et al, 1997;Tohse et al, 2006).…”

Section: Calcium Carbonate Accretionmentioning

confidence: 99%

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Trace Element Patterns in Otoliths: The Role of Biomineralization

Hüssy

Limburg

Pontual

et al. 2020

Reviews in Fisheries Science & Aquaculture

131

120

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Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site.physiological (ontogeny and growth) histories. A consistent temperature response was not evident across studies for either environmental or physiological tracers, presumably attributable to variable relationships between temperature and fish behavior and physiology (e.g., feeding rate, reproduction). Biomineralization thus has a controlling effect on otolith element concentrations for elements that are linked with somatic growth, but not for elements that substitute for Ca in the crystal lattice. Interpretation of the ecological significance of patterns from field samples therefore needs to consider the impact of the underlying biomineralization processes of the element in question as well as physiological processes regulating the availability of ions for inclusion in the growing crystal lattice. Such understanding will enhance the utility of this technique to address fisheries management questions.

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Section: Calcium Carbonate Accretionmentioning

confidence: 99%

“…Immunohistochemical staining of the endolymphatic epithelium revealed that soluble matrix (OMP-1) synthesizing cells occur throughout the transitional and squamous epithelium but not in the sensory epithelium (Takagi and Takahashi, 1999;Takagi et al, 2000;Murayama et al, 2002;Suzuki et al, 2004) (Fig. 2).…”

Section: Soluble Matrixmentioning

confidence: 99%

Trace Element Patterns in Otoliths: The Role of Biomineralization

Hüssy

Limburg

Pontual

et al. 2020

Reviews in Fisheries Science & Aquaculture

131

120

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“…Fish otoliths are primarily composed of calcium carbonate (> 95%) and a small amount of organic matrix. The organic matrix has proteins (Degens et al 1969), carbohydrates (Takagi et al 2000), and their complexes (proteoglycans) as compartments. In the present study, we tested incorporation of organic carbon derived from glucose, but no radioactivity was detected in the organic fraction of otoliths, even though radioactivity of organic carbon in serum was detected 1 h after administration.…”

Section: Discussionmentioning

confidence: 99%

Sources of otolith carbonate: experimental determination of carbon incorporation rates from water and metabolic CO2, and their diel variations

Tohse¹,

Mugiya²

2008

Aquat. Biol.

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Although carbon isotopes in fish otoliths are widely utilized to obtain information on environmental records, uncertainty regarding the sources of otolith carbonate, either from fish diet or ambient water, limits detailed determinations. The present study experimentally determined absolute incorporation rates of carbon derived from 2 sources, metabolic CO 2 and ambient water, into otoliths using goldfish under controlled conditions. In addition, the proportions of the 2 sources in otolith daily increments were also determined by the diel variations in the rate of incorporation from the 2 sources. A group of fish was administered with D-[ 14 C-U]-glucose or was exposed to water containing NaH 14 CO 3 , and incubated for 1, 3, 6, 12 and 24 h. Another group was similarly treated with the radiocarbons at 06:00, 12:00, 18:00, 00:00 or again at 06:00 h, and were incubated for 6 h. After incubation, serum and otoliths were collected and were separated into organic and inorganic carbon fractions, and the incorporation rates from the 2 sources were determined. The rates of carbon incorporation from D-[ 14 C-U]-glucose injected and NaH 14 CO 3 water were 0.27 (25.5%) and 0.79 (74.5%) nmol mg -1 otolith h -1 , respectively. During darkness (18:00 to 06:00 h), both the incorporation rate and proportion of metabolic-CO 2 -derived carbon significantly decreased to ~50% of that during daylight. These results suggest that otolith carbonate is mainly derived from ambient water, and the ratio of carbon derived from metabolic CO 2 is lower in the D-zone than that in the L-zone of the otolith daily increments.

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“…These include the Na + / K + and Ca 2+ ATPases , GP96 , and Carbonic anhydrase ( CA ) isozymes . Other macromolecules, such as carbohydrates, proteoglycans and lipoproteins, have also been detected in the inner ear .…”

Section: Introductionmentioning

confidence: 99%

The inner ear proteome of fish

et al. 2018

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The mechanisms that underpin the formation, growth and composition of otoliths, the biomineralized stones in the inner ear of fish, are largely unknown, as only a few fish inner ear proteins have been reported. Using a partial transcriptome for the inner ear of black bream (Acanthopagrus butcheri), in conjunction with proteomic data, we discovered hundreds of previously unknown proteins in the otolith. This allowed us to develop hypotheses to explain the mechanisms of inorganic material supply and daily formation of growth bands. We further identified a likely protein mediator of crystal nucleation and an explanation for the apparent metabolic inertness of the otolith. Due to the formation of both daily and annual increments, otoliths are routinely employed as natural chronometers, being used for age and growth estimation, fisheries stock assessments, and the reconstruction of habitat use, movement, diet and the impacts of climate change. Our findings provide an unprecedented view of otolith molecular machinery, aiding in the interpretation of these essential archived data.

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Carbohydrates of the otolith organ in the rainbow trout Oncorhynchus mykiss detected by lectins

Cited by 10 publications

References 37 publications

Trace Element Patterns in Otoliths: The Role of Biomineralization

Trace Element Patterns in Otoliths: The Role of Biomineralization

Sources of otolith carbonate: experimental determination of carbon incorporation rates from water and metabolic CO2, and their diel variations

The inner ear proteome of fish

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