Carbon-based hierarchical scaffolds for myoblast differentiation: Synergy between nano-functionalization and alignment

Patel, Akhil; Mukundan, Shilpaa; Wang, Wenhu; Karumuri, Anil Kumar; Sant, Vinayak; Mukhopadhyay, Sharmila M.; Sant, Shilpa

doi:10.1016/j.actbio.2016.01.004

Cited by 58 publications

(43 citation statements)

References 44 publications

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“…In vitro models indicate that muscle progenitor cells utilize alignment cues during myogenesis. 39,40 Similar alignment-sensitive responses have been reported for other cells, including cardiac muscle and tendons. 41,42 Yet, while the many in vitro alignment studies support the motivation of aligned regenerative scaffolds, we recognize that in vivo evidence indicating that scaffold alignment is critical to muscle implant success does not yet exist.…”

Section: Decellularized Skeletal Muscle With Minced Muscle Autograftssupporting

confidence: 51%

Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

Kasukonis

Kim

Brown

et al. 2016

Tissue Engineering Part A

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Skeletal muscle is capable of robust self-repair following mild trauma, yet in cases of traumatic volumetric muscle loss (VML), where more than 20% of a muscle's mass is lost, this capacity is overwhelmed. Current autogenic whole muscle transfer techniques are imperfect, which has motivated the exploration of implantable scaffolding strategies. In this study, the use of an allogeneic decellularized skeletal muscle (DSM) scaffold with and without the addition of minced muscle (MM) autograft tissue was explored as a repair strategy using a lower-limb VML injury model (n = 8/sample group). We found that the repair of VML injuries using DSM + MM scaffolds significantly increased recovery of peak contractile force (81 -3% of normal contralateral muscle) compared to unrepaired VML controls (62 -4%). Similar significant improvements were measured for restoration of muscle mass (88 -3%) in response to DSM + MM repair compared to unrepaired VML controls (79 -3%). Histological findings revealed a marked decrease in collagen dense repair tissue formation both at and away from the implant site for DSM + MM repaired muscles. The addition of MM to DSM significantly increased MyoD expression, compared to isolated DSM treatment (21-fold increase) and unrepaired VML (37-fold) controls. These findings support the further exploration of both DSM and MM as promising strategies for the repair of VML injury.

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Section: Decellularized Skeletal Muscle With Minced Muscle Autograftssupporting

confidence: 51%

Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

Kasukonis

Kim

Brown

et al. 2016

Tissue Engineering Part A

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“…Presence of graphene sheets on the surface (Figure d) may enhance the surface nanoroughness of the nanocomposite fibrous hydrogel films, leading to change in contact angle as well as cell adhesion and spreading. These properties along with fiber alignment at multiple length‐scale could provide contact guidance for the myoblasts to differentiate into myotubes and further, into functional muscle bundles (Patel, Mukundan et al, ). In our previous study, graphene–PCL electrospun scaffolds were shown to stimulate myoblast differentiation, however, these electrospun scaffolds lack multiscale hierarchy and aligned unidirectional fibers (Patel, Xue et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…Surface characterization of fractured hydrogel films was carried out using SEM (Jeol 9335 Field Emission SEM; JEOL, Peabody, MA; Patel, Mukundan et al, ). Fractured hydrogel films were air dried after mechanical testing before SEM imaging.…”

Section: Methodsmentioning

confidence: 99%

“…However, these electrospun nanocomposites did not possess multiscale hierarchy and aligned architecture for contact guidance. In another study, we have demonstrated synergistic effect of nanoroughness, multiscale hierarchy, and aligned fibrous architecture engineered into carbon nanotube‐based hierarchical scaffolds to promote myogenesis (Patel, Mukundan et al, ). These studies from our group have established influence of various biophysical features on myogenesis separately.…”

Section: Introductionmentioning

confidence: 99%

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Hierarchically aligned fibrous hydrogel films through microfluidic self‐assembly of graphene and polysaccharides

Patel

Xue

Hartley

et al. 2018

Biotech & Bioengineering

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Despite significant interest in developing extracellular matrix (ECM)-inspired biomaterials to recreate native cell-instructive microenvironments, the major challenge in the biomaterial field is to recapitulate the complex structural and biophysical features of native ECM. These biophysical features include multiscale hierarchy, electrical conductivity, optimum wettability, and mechanical properties. These features are critical to the design of cell-instructive biomaterials for bioengineering applications such as skeletal muscle tissue engineering. In this study, we used a custom-designed film fabrication assembly, which consists of a microfluidic chamber to allow electrostatic charge-based self-assembly of oppositely charged polymer solutions forming a hydrogel fiber and eventually, a nanocomposite fibrous hydrogel film. The film recapitulates unidirectional hierarchical fibrous structure along with the conductive properties to guide initial alignment and myotube formation from cultured myoblasts. We combined high conductivity, and charge carrier mobility of graphene with biocompatibility of polysaccharides to develop graphene-polysaccharide nanocomposite fibrous hydrogel films. The incorporation of graphene in fibrous hydrogel films enhanced their wettability, electrical conductivity, tensile strength, and toughness without significantly altering their elastic properties (Young's modulus). In a proof-of-concept study, the mouse myoblast cells (C2C12) seeded on these nanocomposite fibrous hydrogel films showed improved spreading and enhanced myogenesis as evident by the formation of multinucleated myotubes, an early indicator of myogenesis. Overall, graphene-polysaccharide nanocomposite fibrous hydrogel films provide a potential biomaterial to promote skeletal muscle tissue regeneration.

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“…The individual fibers range from 5 to 100 um in diameter, and can be as long as several centimeters [50]. In-vitro models indicate that muscle progenitor cells utilize alignment cues during myogenesis [51, 52]. Similar alignment-sensitive responses have been reported for other cells, including cardiac muscle and tendons [53, 54].…”

Section: Discussionmentioning

confidence: 96%

The characterization of decellularized human skeletal muscle as a blueprint for mimetic scaffolds

Wilson

Terlouw

Roberts

et al. 2016

J Mater Sci: Mater Med

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The use of decellularized skeletal muscle (DSM) as a cell substrate and scaffold for the repair of volumetric muscle loss injuries has shown therapeutic promise. The performance of DSM materials motivated our interest in exploring the chemical and physical properties of this promising material. We suggest that these properties could serve as a blueprint for the development of next generation engineered materials with DSM mimetic properties. In this study, whole human lower limb rectus femoris (n=10) and upper limb supraspinatus muscle samples (n=10) were collected from both male and female tissue donors. Skeletal muscle samples were decellularized and nine property values, capturing key compositional, architectural, and mechanical properties, were measured and statistically analyzed. Mean values for each property were determined across muscle types and genders. Additionally, the influence of muscle type (upper versus lower limb) and donor gender (male versus female) on each of the DSM material properties was examined. The data suggests that DSM materials prepared from lower limb rectus femoris samples have an increased modulus and contain a higher collagen content then upper limb supraspinatus muscles. Specifically, lower limb rectus femoris DSM material modulus and collagen content was approximately twice that of lower limb supraspinatus DSM samples. While muscle type did show some influence on material properties, we did not find significant trends related to gender. The material properties reported herein may be used as a blueprint for the data-driven design of next generation engineered scaffolds with muscle mimetic properties, as well as inputs for computational and physical models of skeletal muscle.

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Carbon-based hierarchical scaffolds for myoblast differentiation: Synergy between nano-functionalization and alignment

Cited by 58 publications

References 44 publications

Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

Hierarchically aligned fibrous hydrogel films through microfluidic self‐assembly of graphene and polysaccharides

The characterization of decellularized human skeletal muscle as a blueprint for mimetic scaffolds

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