Carbon-Based Nanoparticles and Their Surface-Modified Counterparts as MALDI Matrices

Khajavinia, Amir; El‐Aneed, Anas

doi:10.1021/acs.analchem.2c04537

Cited by 14 publications

(2 citation statements)

References 207 publications

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“…Moreover, an ideal matrix has few matrix-related ion signals, strong absorption at the laser operating wavelength, and high ionization efficiency for the analytes . Currently, it is worth mentioning that inorganic nanomaterial matrices, such as platinum nanomaterial, and carbon-based nanoparticles have attracted much attention due to their few background ions . Nevertheless, the drawbacks of inorganic matrices are also obvious, such as low sensitivity, poor reproducibility, high cost, and some damage to the mass spectrometers .…”

Section: Introductionmentioning

confidence: 99%

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Bao,

Yu,

et al. 2024

Anal. Chem.

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Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which is a label-free imaging technique, determines the spatial distribution and relative abundance of versatile endogenous metabolites in tissues. Meanwhile, matrix selection is generally regarded as a pivotal step in MALDI tissue imaging. This study presents the first report of a novel MALDI matrix, 2-hydroxy-5-nitro-3-(trifluoromethyl)pyridine (HNTP), for the in situ detection and imaging of endogenous metabolites in rat liver and brain tissues by MALDI-MS in positive-ion mode. The HNTP matrix exhibits excellent characteristics, including strong ultraviolet absorption, μm-scale matrix crystals, high chemical stability, low background ion interference, and high metabolite ionization efficiency. Notably, the HNTP matrix also shows superior detection capabilities, successfully showing 185 detectable metabolites in rat liver tissue sections. This outperforms the commonly used matrices of 2,5-dihydroxybenzoic acid and 2-mercaptobenzothiazole, which detect 145 and 120 metabolites from the rat liver, respectively. Furthermore, a total of 152 metabolites are effectively detected and imaged in rat brain tissue using the HNTP matrix, and the spatial distribution of these compounds clearly shows the heterogeneity of the rat brain. The results demonstrate that HNTP is a new and powerful positive-ion mode matrix to enhance the analysis of metabolites in biological tissues by MALDI-MSI.

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Section: Introductionmentioning

confidence: 99%

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Bao,

Yu,

et al. 2024

Anal. Chem.

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show abstract

“…To assist in transferring the light energy received by the surface to the analyte, the surface nanomaterial for SALDI should have excellent photoabsorption properties. Currently, nanostructured substrates consisting of gold , and silver nanoparticles, titania, silica, , and carbon-based nanomaterials effectively increase the laser desorption ionization (LDI) efficiency of analytes.…”

Section: Introductionmentioning

confidence: 99%

Graphite Sheet-Assisted Laser Desorption Ionization-Mass Spectrometry for Small Organic Compound Analysis

Liu,

Arima,

Nishiki

et al. 2024

ACS Omega

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Doped nanomaterial facilitates 3D printing target plate for rapid detection of alkaloids in laser desorption/ionization mass spectrometry

Wang,

Qin,

et al. 2023

Anal Bioanal Chem

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Carbon-Based Nanoparticles and Their Surface-Modified Counterparts as MALDI Matrices

Cited by 14 publications

References 207 publications

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Graphite Sheet-Assisted Laser Desorption Ionization-Mass Spectrometry for Small Organic Compound Analysis

Doped nanomaterial facilitates 3D printing target plate for rapid detection of alkaloids in laser desorption/ionization mass spectrometry

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